We narrowed to 13,735 results for: TIM;

1. pYFP-lacY-1

   Plasmid

   #140138

   Purpose

   Evaluating the impact of feedback on information transmission of a genetic sensor.

   Depositor

   Drew Tack

   Article

   Sarkar et al Commun Biol. 2020 Apr 30;3(1):203. doi:

   Insert

   lacY (lacY )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHFX-cas3UD

   Plasmid

   #174385

   Purpose

   Plasmids for gene knockout were constructed based on suicide plasmid pHFX

   Depositor

   Jing Han

   Article

   Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

   Insert

   Cas3UD
   

   Use

   Archaeal gene knockout vector for haloferax medit…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMX Vav1 Y3F/PH* (W495L) uGFP

   Plasmid

   #14564

   Depositor

   Joan Brugge

   Article

   Wilsbacher et al Cell Commun Signal. 2006 . 4():5.

   Insert

   Vav1 (VAV1 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   PH* (W495L)

   Promoter

   Available since

   April 12, 2007

   Availability

   Academic Institutions and Nonprofits only

4. pMX Vav1 Y3F/CR* (C529S) uGFP

   Plasmid

   #14560

   Depositor

   Joan Brugge

   Article

   Wilsbacher et al Cell Commun Signal. 2006 . 4():5.

   Insert

   Vav1 (VAV1 Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Y3F/CR* (C529S)

   Promoter

   Available since

   April 12, 2007

   Availability

   Academic Institutions and Nonprofits only

5. OeNL-H2B_pcDNA3

   Plasmid

   #89527

   Purpose

   Orange color variant of bright luminescent protein, fused to histone 2B

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   OeNL
   

   Use

   Tags

   histone 2B

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSox2-bd::FP

   Plasmid

   #34703

   Depositor

   Hollis Cline

   Article

   Bestman et al J Comp Neurol. 2012 Feb 1;520(2):401-33.

   Insert

   eGFP
   

   Use

   Xenopus expression

   Tags

   165-bp minimal FGF4 promoter, 6x Sox2/Oct3-4 tran…

   Expression

   Mutation

   Promoter

   Available since

   Feb. 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC19-U6-crRNA1

   Plasmid

   #123639

   Purpose

   Mammalian expression, Genome editing, gRNA scaffold

   Depositor

   Wei Li

   Article

   Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

   Insert

   crRNA scaffold 1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. RV-Syn-Dio-GFP

   Plasmid

   #87673

   Purpose

   retroviral vector encoding cre dependent expression of GFP

   Depositor

   Oscar Marin

   Article

   Lim et al Nat Neurosci. 2018 Jun 18. pii: 10.1038/

   Insert

   eGFP
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Synapsin

   Available since

   May 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAW8ENdeI-cHAIAA7-spo5DSR

   Plasmid

   #49873

   Purpose

   for Cre-lox cassette exchange of C-terminal HA-auxin-inducible degron tagged gene sequences together with the S. pombe spo5 gene DSR element into the S. pombe urg1 locus

   Depositor

   Tony Carr

   Article

   Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

   Type

   Empty backbone

   Use

   Cre/Lox

   Tags

   IAA17 degron tag

   Expression

   Mutation

   Promoter

   Available since

   Jan. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. KA1130_pPB-TREtight-cHA-IRESH2BmCherry

    Plasmid

    #124173

    Purpose

    piggyBac-based Dox-inducible expression

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLentiCRISPRv1.linker_KO

    Plasmid

    #164688

    Purpose

    For CRISPR knockout of miR-144~451 linker region by lentiviral delivery of Cas9 and linker gRNA

    Depositor

    David Bartel

    Article

    Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

    Insert

    miR-144~451 linker CRISPR KO gRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSUPER-retro-puro-shNup133-2

    Plasmid

    #87333

    Purpose

    To express shRNA against human Nup133

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    shRNA against human Nup133
    

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. OeNL-Actin_pcDNA3

    Plasmid

    #89524

    Purpose

    Orange color variant of bright luminescent protein, fused to actin

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    OeNL
    

    Use

    Tags

    Actin

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pMOS030: ratiometric pHluorin (endoplasmic reticulum)

    Plasmid

    #163054

    Purpose

    ratiometric pHluorin (endoplasmic reticulum)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    ratiometric pHluorin
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pNB774

    Plasmid

    #60114

    Purpose

    Green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA)

    Depositor

    Nicolas Buchler

    Article

    Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

    Insert

    GrLuc
    

    Use

    Unspecified

    Tags

    ClpXP degron and Nuclear localization signal (NLS…

    Expression

    Mutation

    Promoter

    Available since

    Oct. 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJ414 - DANCER-3

    Plasmid

    #136387

    Purpose

    Expresses DANCER-3 dynamic protein

    Depositor

    Roberto Chica

    Article

    Davey et al Nat Chem Biol. 2017 Dec;13(12):1280-1285

    Insert

    DANCER-3
    

    Use

    Tags

    6x-His tag (N-term of insert)

    Expression

    Bacterial

    Mutation

    Mutation from GB1 WT: Y3F-L7I-A34F-V39L-V54I

    Promoter

    T7 promoter

    Available since

    Oct. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRSET FLII32Pglu-2.2m

    Plasmid

    #13566

    Depositor

    Wolf Frommer

    Article

    Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

    Insert

    FLIPglu F16A P32S-ECFP-D33R
    

    Use

    Tags

    6xHis and Citrine

    Expression

    Bacterial

    Mutation

    F16A, ECFP insert in binding protein

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pUCBB-lacZa

    Plasmid

    #32563

    Depositor

    Claudia Schmidt-dannert

    Article

    Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

    Insert

    LacZ-alpha
    

    Use

    Synthetic Biology

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    Consitutive lac

    Available since

    Nov. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. KA2706_eSpCas9-PGKPacdtkCh

    Plasmid

    #124204

    Purpose

    Expression of eSpCas9(1.1) and sgRNA

    Depositor

    Hans Schöler

    Article

    Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pYFP-x-1

    Plasmid

    #140142

    Purpose

    Evaluating information transmission of a genetic sensor.

    Depositor

    Drew Tack

    Article

    Sarkar et al Commun Biol. 2020 Apr 30;3(1):203. doi:

    Insert

    lacY (lacY )
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    D37, I38, and N39 are changed to nonsense (stop) …

    Promoter

    Available since

    April 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge