We narrowed to 9,602 results for: FIE
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Plasmid#107471PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation G484E decreased transport and surface expression relative to wildtypeDepositorInsertSerotonin Transporter (SERT) (SLC6A4 Human)
UseTagsExpressionMammalianMutationG484EPromoterCMVAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTS477F-myc
Plasmid#107470PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation S477F loss of transport relative to wildtype, decreased surface expressionDepositorInsertSerotonin Transporter (SERT) (SLC6A4 Human)
UseTagsExpressionMammalianMutationS477FPromoterCMVAvailable sinceMarch 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-Y100F
Plasmid#110826PurposeBacterial expression of E.coli DHFR Y100F mutantDepositorInsertecDHFR
UseTagsExpressionBacterialMutationY100 mutated to F100PromoterT7Available sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-BE3-3NLS
Plasmid#119032PurposeStably express BE3 in mammalian cellsDepositorInsertBE3
UseTags3xFlagExpressionMammalianMutationPromoterAvailable sinceJan. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
RCAS(A) human p110 alpha E545K
Plasmid#52211Purposeexpresses human p110 alpha with E545K mutation in avian cellsDepositorInsertp110 alpha E545K (PIK3CA Human)
UseRetroviral; AvianTagsExpressionMutationGlutamic Acid 545 to LysinePromoterAvailable sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
p3E-Dnm2b-DN
Plasmid#109574PurposeMultisite gateway vector for 3' tagging with dominant negative Dynamin2bDepositorInsertDynamin2b (dnm2b Zebrafish)
UseZebrafish plasmidsTagsExpressionMutationdominant negativePromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-EHD1b-dEH
Plasmid#109567PurposeMultisite gateway vector for 3' tagging with EHD1b-delta-EHDepositorInsertEHD1b (ehd1b Zebrafish)
UseZebrafish plasmidsTagsExpressionMutationdelta-EHPromoterAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
Tmem194 pmRFP-N2 (1389)
Plasmid#62048Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertTmem194 (TMEM194A Human)
UseTagsmRFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-SERTI425V-myc
Plasmid#107464PurposeMammalian expression plasmid for codon optimized mutant hSERT with internal MYC tag inserted in ECL2, mutation I425V conferring aproximately wildtype phenotypeDepositorInsertSerotonin Transporter (SERT) (SLC6A4 Human)
UseTagsExpressionMammalianMutationI425VPromoterCMVAvailable sinceMarch 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
sh-Myocardin
Plasmid#100768Purposeexpression of shRNA targeting MYOCDDepositorInsertsh-Myocardin
UseRNAiTagsExpressionMutationPromoterH1Available sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
Tmem194 pEGFP-N2 (1074)
Plasmid#62027Purposemammalian expression of nuclear envelope transmembrane proteinDepositorInsertTmem194 (TMEM194A Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSC-hTDG-K330A-IntE2
Plasmid#52271PurposeBacterial expression of human TDG-K330A C-terminally fused to GyrA intein, SUMO-E2 and GSTDepositorInserthTDG (TDG Human)
UseTagsGyrA-intein-Ubc9-GSTExpressionBacterialMutationK330A, SUMO acceptor site mutationPromoterT7Available sinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSC-mTDG-IntE2
Plasmid#52268PurposeBacterial expression of mouse TDG C-terminally fused to GyrA intein, SUMO-E2 and GSTDepositorInsertTDG (Tdg Mouse)
UseTagsGSTExpressionBacterialMutationPromoterT7Available sinceJuly 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJH124
Plasmid#48259PurposeTemplate plasmid for PCR-based transplant of Ashbya gossypii TEF1 promoter in yeast.DepositorInsertsURA3 3' fragment
URA3 5' fragment
Ag TEF1 promoter
UseRoutine cloning vectorTagsExpressionMutation-242 upstream to URA3 ORF +495 and URA3 ORF from …PromoterAg TEF1 promoterAvailable sinceFeb. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNRCreCNAT
Plasmid#234430PurposePicochlorum celeri CloNat insert with nitrate-inducible Cre recombinase and loxP sites for marker and Cre removalDepositorInsertCre recombinase
UseCRISPR, Cre/Lox, and Synthetic Biology ; Green al…TagsExpressionMutationPicochlorum celeri endoglucanase intron insertionPromoterP celeri nitrate reductaseAvailable sinceAug. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNRCreBle
Plasmid#234431PurposePicochlorum celeri ShBle insert with nitrate-inducible Cre recombinase and loxP sites for marker and Cre removalDepositorInsertCre recombinase
UseCRISPR, Cre/Lox, and Synthetic Biology ; Green al…TagsExpressionMutationPicochlorum celeri endoglucanase intron insertionPromoterP celeri nitrate reductaseAvailable sinceAug. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
SAGA046
Plasmid#240795PurposePlasmid contains the triple selection cassette gfp -pBR322ROP tetApBR322ROP - CmTrunc that allows for in vivo recombineering in E.coli.DepositorInsertSAGA pBR322ROP His medium : T7- His tags medium- gfp -pBR322ROP tetApBR322ROP - CmTrunc
UseSynthetic BiologyTagsExpressionMutationMutation on translation initiation region of tetAPromoterAvailable sinceAug. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
SAGA045
Plasmid#240794PurposePlasmid contains the triple selection cassette gfp -pBR322ROP tetApBR322ROP - CmTrunc that allows for in vivo recombineering in E.coli.DepositorInsertSAGA pBR322ROP His low : T7- His tags low - gfp -pBR322ROP tetApBR322ROP - CmTrunc
UseSynthetic BiologyTagsExpressionMutationMutation on translation initiation region of tetAPromoterAvailable sinceAug. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
SAGA043
Plasmid#240792PurposePlasmid contains the triple selection cassette gfp -p15a tetAp15a - CmTrunc that allows for in vivo recombineering in E.coli.DepositorInsertSAGA p15a His medium : T7- His tags medium- gfp -p15a tetAp15a - CmTrunc
UseSynthetic BiologyTagsExpressionMutationMutation on translation initiation region of tetAPromoterAvailable sinceAug. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
SAGA042
Plasmid#240791PurposePlasmid contains the triple selection cassette gfp -p15a tetAp15a - CmTrunc that allows for in vivo recombineering in E.coli.DepositorInsertSAGA p15a His low : T7- His tags low - gfp -p15a tetAp15a - CmTrunc
UseSynthetic BiologyTagsExpressionMutationMutation on translation initiation region of tetAPromoterAvailable sinceAug. 20, 2025AvailabilityAcademic Institutions and Nonprofits only