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INT_Cargo_T7_CbFDH

Plasmid

#212140

Purpose

Cargo plasmid for integrating PT7 expression cassette of formate dehydrogenase in genome. Plasmid can be removed by incubating cells at 37 C.

Depositor

Kang Zhou

Article

Fung et al Biomaterials. 2022 Aug;287:121661. doi:

Insert

formate dehydrogenase

Expression

Bacterial

Promoter

PT7

Available Since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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CRISPR-psbA2 point mutation

Plasmid

#182929

Purpose

point mutation on psbA2 by CRISPR in Synechocystis 6803

Depositor

Himadri Pakrasi

Article

Ungerer et al Sci Rep. 2016 Dec 21;6:39681. doi: 10.10

Inserts

ddcpf1
gRNA targeting psbA2 in Synechocystis 6803: gatcttcggtcgcttgatctttc
psbA

Use

CRISPR

Mutation

S264A, and silent mutation to remove PAM site

Available Since

April 7, 2022

Availability

Academic Institutions and Nonprofits only

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pJL32

Plasmid

#174066

Purpose

Encodes for CIB1 fused to VP16 in S. cerevisiae. Part of optogenetic system.

Depositor

Albert Keung

Article

Lee et al Cell Syst. 2021 Aug 24. pii: S2405-4712(

Insert

pGal1-LacO-3NLS-CIB1-VP16-tADH1

Tags

FLAG

Expression

Yeast

Promoter

pGal1

Available Since

March 28, 2022

Availability

Academic Institutions and Nonprofits only

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BPK617

Plasmid

#179297

Purpose

Expresses dCas9 fused to VP64

Depositor

Keith Joung

Article

Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

Insert

dCas9-VP64

Expression

Mammalian

Mutation

D10A, H840A (catalytically inactive Cas9)

Promoter

CAG

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pJL30

Plasmid

#174064

Purpose

Encodes for 43-8 ZF fused to Cry2. Used for optogenetic recruitment of TF. Used with pJL32.

Depositor

Albert Keung

Article

Lee et al Cell Syst. 2021 Aug 24. pii: S2405-4712(

Insert

pGal1-TetO-3xNLS-43-8ZF-Cry2-tADH1

Tags

FLAG

Expression

Yeast

Promoter

pGal1

Available Since

Dec. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pJL15

Plasmid

#174069

Purpose

Expresses 43-8 zinc finger array fused to CRY2 in S. cerevisiae. Part of optogenetic system.

Depositor

Albert Keung

Article

Lee et al Cell Syst. 2021 Aug 24. pii: S2405-4712(

Insert

pGAL-tetO-3xNLS-43-8WT-GS-Cry2-Tadh1

Tags

FLAG

Expression

Yeast

Promoter

pGal1

Available Since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pRS41K-mCherry-mNeonGreen (pJJF003)

Plasmid

#173459

Purpose

C-SWAT donor for Seamless tagging, contains mCherry-mNeonGreen tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Article

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mCherry-mNeonGreen

Expression

Yeast

Promoter

no promoter

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pJBL714

Plasmid

#140384

Purpose

Expresses CtcS-regulated 3WJdB RNA aptamer driven by T7 promoter with the ctcO sequence 5BP downstream from the promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-ctcO-3WJdB-T

Use

Synthetic Biology

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pJBL720

Plasmid

#140390

Purpose

Expresses QacR-regulated 3WJdB RNA aptamer driven by T7 promoter with the qacO sequence 5BP downstream from the promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-qacO-3WJdB-T

Use

Synthetic Biology

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pJBL722

Plasmid

#140392

Purpose

Expresses TtgR-regulated 3WJdB RNA aptamer driven by T7 promoter with the ttgO sequence 5BP downstream from the promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-ttgO-3WJdB-T

Use

Synthetic Biology

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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psiCHECK3-TMED3-Reverse-Complement

Plasmid

#136018

Purpose

The Reverse Complement of TMED3 3'UTR inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

TMED3 3'UTR (Reverse Complement) (TMED3 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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psiCHECK3-ZC3H15-Reverse-Complement

Plasmid

#136019

Purpose

The Reverse Complement of ZC3H15 3'UTR inserted into the 3'UTR of Renilla luciferase in the psiCHECK3 plasmid

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

ZC3H15 3'UTR (Reverse Complement) (ZC3H15 Human)

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pSKB3SaGGR I206F (JBEI-3678)

Plasmid

#110149

Purpose

SaGGR site-directed mutation of I206F

Depositor

Jay Keasling

Article

Kung et al Structure. 2014 Jul 8;22(7):1028-36. doi

Insert

GGR

Expression

Bacterial

Mutation

SaGGR site-directed mutation of I206F

Promoter

T7

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pSKB3SaGGR G91H (JBEI-3677)

Plasmid

#110148

Purpose

SaGGR site-directed mutation of G91H

Depositor

Jay Keasling

Article

Kung et al Structure. 2014 Jul 8;22(7):1028-36. doi

Insert

GGR

Expression

Bacterial

Mutation

SaGGR site-directed mutation of G91H

Promoter

T7

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pSKB3SaGGR L377H (JBEI-3683)

Plasmid

#110150

Purpose

SaGGR site-directed mutation of L377H

Depositor

Jay Keasling

Article

Kung et al Structure. 2014 Jul 8;22(7):1028-36. doi

Insert

GGR

Expression

Bacterial

Mutation

SaGGR site-directed mutation of L377H

Promoter

T7

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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CMYA5/pBSSK+

Plasmid

#97205

Purpose

CMYA5 (NM_153610, nt 12205 to 12279) in pBSSK+

Depositor

Rita Shiang

Article

Hsiung et al J Psychiatr Res. 2019 Apr;111:8-15. doi:

Insert

CMYA5 (CMYA5 Human)

Use

Unspecified

Available Since

July 28, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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CMYA5/pENTR3C

Plasmid

#97408

Purpose

CMYA5 (NM_153610, nt 12205 to 12279) in pENTR3C, Gateway Entry vector

Depositor

Rita Shiang

Article

Hsiung et al J Psychiatr Res. 2019 Apr;111:8-15. doi:

Insert

CMYA5 (CMYA5 Human)

Use

Unspecified

Available Since

July 18, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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LGhG-pHG165a

Plasmid

#90048

Purpose

Constitutive expression of a chimeric LacI:GalR transcription repressor

Depositor

Liskin Swint-Kruse

Article

Tungtur et al J Mol Biol. 2010 Jan 29;395(4):785-802.

Insert

LGhG

Expression

Bacterial

Promoter

Iq

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pNCMV HPV8 E6 R138S

Plasmid

#87659

Purpose

Flag/HA tagged HPV8 E6 R138S

Depositor

Karl Munger

Article

Meyers et al PLoS Pathog. 2017 Jan 20;13(1):e1006171.

Insert

HPV 8E6

Tags

Flag and HA

Expression

Mammalian

Mutation

R138S

Promoter

CMV

Available Since

June 1, 2017

Availability

Academic Institutions and Nonprofits only

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pLentiN HPV8 E6 K142A

Plasmid

#87680

Purpose

Lentiviral expression of HPV8 E6 K142A

Depositor

Karl Munger

Article

Meyers et al PLoS Pathog. 2017 Jan 20;13(1):e1006171.

Insert

HPV 8E6

Use

Lentiviral

Tags

Flag and HA

Mutation

K142A

Promoter

CMV

Available Since

March 14, 2017

Availability

Academic Institutions and Nonprofits only

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