We narrowed to 14,882 results for: TIM;

1. pMS051

   Plasmid

   #137025

   Purpose

   SapTrap mScarlet-I-GLO donor

   Depositor

   Daniel Dickinson, Bob Goldstein

   Insert

   C. elegans GLO-optimized mScarlet
   

   Use

   CRISPR; Saptrap donor for cloning repair templat…

   Tags

   C. elegans GLO-optimized mScarlet

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDD346

   Plasmid

   #133311

   Purpose

   SapTrap mNG-C1 donor

   Depositor

   Daniel Dickinson

   Insert

   C. elegans codon-optimized mNeonGreen
   

   Use

   CRISPR; Saptrap donor for cloning repair templat…

   Tags

   C. elegans codon-optimized mNeonGreen

   Expression

   Worm

   Mutation

   Promoter

   None

   Available since

   Nov. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDD373

   Plasmid

   #137021

   Purpose

   SapTrap GFP-GLO donor

   Depositor

   Daniel Dickinson, Bob Goldstein

   Insert

   C. elegans GLO-optimized GFP
   

   Use

   CRISPR; Saptrap donor for cloning repair templat…

   Tags

   C. elegans GLO-optimized GFP

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLSU2/mIR{PepGMV}:mNeonGreen:NosT

   Plasmid

   #212193

   Purpose

   This binary vector expresses mNeonGreen with a modified IR pepper golden mosaic virus (PepGMV) promoter

   Depositor

   Wayne Curtis

   Insert

   Tomato codon optimized version of mNeonGreen
   

   Use

   Tags

   Expression

   Plant

   Mutation

   mNeonGreen codon-optimized for tomato

   Promoter

   PepGMV mIR promoter

   Available since

   March 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLSU1/t35S[TMV]:mNeonGreen:NosT

   Plasmid

   #212162

   Purpose

   This binary vector expresses mNeonGreen with a truncated 35sCAMV promoter with strong UTR (Tomato Mosaic Virus)

   Depositor

   Wayne Curtis

   Insert

   Tomato codon optimized version of mNeonGreen
   

   Use

   Tags

   Expression

   Plant

   Mutation

   mNeonGreen codon-optimized for tomato

   Promoter

   truncated 35sCaMV

   Available since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDD376

   Plasmid

   #137022

   Purpose

   SapTrap mKate2-GLO donor

   Depositor

   Daniel Dickinson, Bob Goldstein

   Insert

   C. elegans GLO-optimized mKate2
   

   Use

   CRISPR; Saptrap donor for cloning repair templat…

   Tags

   C. elegans GLO-optimized mKate2

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. C108m

   Plasmid

   #121010

   Purpose

   MoClo golden gate assembly CD part for mScarlet-I-pdt3 (fast-folding mScarlet variant, codon-optimized for E. col, with pdt3 degradation tag; see https://www.fpbase.org/protein/mscarlet-i/).

   Depositor

   Richard Murray

   Insert

   mScarlet-I codon optimized with pdt3 tag
   

   Use

   Synthetic Biology

   Tags

   pdt3 mfLon degradation tag

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. C103m

   Plasmid

   #121007

   Purpose

   MoClo golden gate assembly CD part for mScarlet-I-LAA (fast-folding mScarlet variant, codon-optimized for E. col, with LAA degradation tag; see https://www.fpbase.org/protein/mscarlet-i/).

   Depositor

   Richard Murray

   Insert

   mScarlet-I codon optimized with LAA tag
   

   Use

   Synthetic Biology

   Tags

   LAA degradation tag for ssrA

   Expression

   Mutation

   Promoter

   Available since

   Dec. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCVD450

   Plasmid

   #66153

   Purpose

   cloning of E coli E2348/69 PerA

   Depositor

   James Kaper

   Article

   Gomez et al University of Maryland, Baltimore. Disse

   Insert

   perA
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   none

   Available since

   Sept. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pGR595

    Plasmid

    #213785

    Purpose

    Integrates at the CAN1 locus the BadBoy2 TP-DNAP1 variant and the Nourseothricin resistance marker. Includes an I-SceI transient expression cassette.

    Depositor

    Chang Liu

    Insert

    BadBoy2 TP-DNAP1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. YPet-N1

    Plasmid

    #54637

    Purpose

    Localization: N1 Cloning Vector, Excitation: 513, Emission: 527

    Depositor

    Patrick Daugherty, Michael Davidson

    Article

    Nguyen et al Nat Biotechnol. 2005 Mar . 23(3):355-60.

    Type

    Empty backbone

    Use

    Tags

    YPet

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pLexm

    Plasmid

    #99844

    Purpose

    mammalian expression

    Depositor

    Edith Yvonne Jones

    Article

    Aricescu et al Acta Crystallogr D Biol Crystallogr. 200

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDD362

    Plasmid

    #137023

    Purpose

    SapTrap HaloTag-GLO donor

    Depositor

    Daniel Dickinson, Bob Goldstein

    Insert

    C. elegans GLO-optimized Halo Tag
    

    Use

    CRISPR; Saptrap donor for cloning repair templat…

    Tags

    C. elegans GLO-optimized Halo Tag

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. YPet-C1

    Plasmid

    #54648

    Purpose

    Localization: C1 Cloning Vector, Excitation: 513, Emission: 527

    Depositor

    Patrick Daugherty, Michael Davidson

    Article

    Nguyen et al Nat Biotechnol. 2005 Mar . 23(3):355-60.

    Type

    Empty backbone

    Use

    Tags

    YPet

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

15. CYPet-N1

    Plasmid

    #54638

    Purpose

    Localization: N1 Cloning Vector, Excitation: 433, Emission: 475

    Depositor

    Patrick Daugherty, Michael Davidson

    Article

    Nguyen et al Nat Biotechnol. 2005 Mar . 23(3):355-60.

    Type

    Empty backbone

    Use

    Tags

    CYPet

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. CYPet-C1

    Plasmid

    #54649

    Purpose

    Localization: C1 Cloning Vector, Excitation: 433, Emission: 475

    Depositor

    Patrick Daugherty, Michael Davidson

    Article

    Nguyen et al Nat Biotechnol. 2005 Mar . 23(3):355-60.

    Type

    Empty backbone

    Use

    Tags

    CYPet

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

17. pGEX4T1-WWP1F792D

    Plasmid

    #179328

    Purpose

    E. coli optimized expression of GST-fusion of human WWP1 F792D

    Depositor

    Arthur Haas

    Insert

    WWP1 F792D (WWP1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    F792D mutation; Codon Optimized for expression in…

    Promoter

    Available since

    April 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAIP4

    Plasmid

    #233686

    Purpose

    Cloning vector with an N-terminal fusion of 10x His-tagged SUMO-1, followed by a Golden Gate assembly site for seamless insertion.

    Depositor

    Christopher Bahl

    Insert

    Smt3 (SMT3 )
    

    Use

    Tags

    HisTag

    Expression

    Mutation

    Promoter

    T7

    Available since

    April 7, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. Cas9_P2A_T4pol

    Plasmid

    #190146

    Purpose

    Expresses SpCas9 and T4pol

    Depositor

    Timothy Lu

    Article

    Nakade et al bioRxiv 2022.12.05.518807

    Insert

    SpCas9_P2A_T4pol
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Codon Optimized for mammalian cells

    Promoter

    CBh

    Available since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSaCas9_GFP

    Plasmid

    #64709

    Purpose

    Co-expression of human codon-optimized Staphylococcus aureus Cas9 nuclease and GFP, plasmid optimized for expression in human pluripotent stem cells and other mammalian cells

    Depositor

    Kiran Musunuru

    Insert

    SaCas9-2A-GFP
    

    Use

    CRISPR

    Tags

    2A-GFP and 3xFLAG-SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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