Addgene

We narrowed to 17,138 results for: STO

Showing: 2421 - 2440 of 17138 results

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THI VP64 pGAP T5

Plasmid

#161492

Purpose

CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T5_gRNA_MS2

Depositor

Brigitte Gasser, Diethard Mattanovich

Article

Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

Inserts

dCAS9
MS2-VP64
gRNA (Targeting T5 in the THI11 promoter)

Use

Tags

Expression

Yeast

Mutation

Promoter

pGAP, pPOR1, and pTEF2

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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THI VP64 pGAP T1

Plasmid

#161488

Purpose

CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T1_gRNA_MS2

Depositor

Brigitte Gasser, Diethard Mattanovich

Article

Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3

Inserts

dCAS9
MS2-VP64
gRNA (Targeting T1 in the THI11 promoter)

Use

Tags

Expression

Yeast

Mutation

Promoter

pGAP, pPOR1, and pTEF2

Available since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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phNFL-Q332P

Plasmid

#132599

Purpose

Expresses human NFL containing the Charcot-Marie-Tooth disease type 2E Q332P mutation

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

Nefl (Nefl Human)

Use

Tags

Expression

Mammalian

Mutation

995A>C;Gln332Pro

Promoter

CMV

Available since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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Let7R-IRE-TREAT

Plasmid

#196934

Purpose

Reverse complement of let7-miRNA binding sites (3x) were integrated in between the stop codon and TREAT cassette in IRE-TREAT.

Depositor

Jeffrey Chao

Article

Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

Insert

Renilla luciferase

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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BB3aN_14*

Plasmid

#98548

Purpose

empty BB3 for insertion and overexpression of 1 gene in P. pastoris

Depositor

Brigitte Gasser, Diethard Mattanovich, Michael Sauer

Article

Prielhofer et al BMC Syst Biol. 2017 Dec 8;11(1):123. doi

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pGL3-basic-hFX-promotor(2,5kb)

Plasmid

#14980

Depositor

Michael Ristow

Insert

human frataxin promoter fragment (FXN Human)

Use

Luciferase

Tags

Expression

Mutation

start codon for human frataxin gene has been muta…

Promoter

Available since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
pAAV-hSyn-ChrimsonR-LSSmOrange

Plasmid

#149691

Purpose

For packaging and expression of ChrimsonR-LSSmOrange in neurons via AAV

Depositor

Robert Malenka

Article

Christoffel et al Proc Natl Acad Sci U S A. 2021 Jun 15;11

Insert

ChrimsonR

Use

AAV

Tags

LSSmOrange

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pHB4

Plasmid

#53462

Purpose

This plasmid contains an ADHpr driven chimeric protein with SNC1, N-terminal GFP and C-terminal SUC2. It contains flanking homology to SUC2, allowing optional integration.

Depositor

Elizabeth Conibear

Article

Burston et al J Cell Biol. 2009 Jun 15. 185(6):1097-11

Insert

yeGFP-Snc1-Suc2

Use

Tags

Expression

Yeast

Mutation

Promoter

ADH

Available since

May 19, 2016

Availability

Academic Institutions and Nonprofits only

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pTrcHIS-7D12Gln13TAG

Plasmid

#125264

Purpose

Expresses 7D12 anti-EGFR nanobodies with UAG codon at Gln13 for synthetic amino acid in combination with pEVOL-pAzF

Depositor

Angus Johnston

Article

Yong et al Nano Lett. 2019 Mar 13;19(3):1827-1831.

Insert

Gln13TAG 7D12

Use

Tags

His-Tag

Expression

Bacterial

Mutation

Promoter

Available since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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hisSUMO-mZFP57

Plasmid

#87224

Purpose

Express hisSUMO tagged Zinc finger protein 57 F1-F3(mouse) in E. coli

Depositor

Gary Stormo

Article

Zuo et al Sci Adv. 2017 Nov 17;3(11):eaao1799. doi

Insert

ZFP57 (Zfp57 Mouse)

Use

Tags

hisSUMO

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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prNFL-P8R-EGFP

Plasmid

#132598

Purpose

Expresses EGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E P8R mutation

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

Nefl (Nefl Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

23C>G; Pro8Arg

Promoter

CMV

Available since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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prNFL-N98S-EGFP

Plasmid

#132600

Purpose

Expresses EGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E N98S mutation

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

Nefl (Nefl Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

293A>G; Asn98Ser

Promoter

CMV

Available since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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prNFL-E397K-EGFP

Plasmid

#132603

Purpose

Expresses EGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E E397K mutation

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

Nefl (Nefl Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

1189G>A; Glu397Lys

Promoter

CMV

Available since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pGL3-basic-hFX-promotor(1,3kb)

Plasmid

#14979

Depositor

Michael Ristow

Insert

human frataxin promoter fragment (FXN Human)

Use

Luciferase

Tags

Expression

Mutation

start codon for human frataxin gene has been muta…

Promoter

Available since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
pTrcHis-7D12CTermTAG

Plasmid

#125267

Purpose

Expresses 7D12 anti-EGFR nanobodies with UAG codon at c-terminus for synthetic amino acid in combination with pEVOL-pAzF

Depositor

Angus Johnston

Article

Yong et al Nano Lett. 2019 Mar 13;19(3):1827-1831.

Insert

CTermTAG

Use

Tags

His-Tag

Expression

Mutation

Promoter

Trc

Available since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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pTrcHis-7D12Gly42TAG

Plasmid

#125265

Purpose

Expresses 7D12 anti-EGFR nanobodies with UAG codon at Gly42 for synthetic amino acid in combination with pEVOL-pAzF

Depositor

Angus Johnston

Article

Yong et al Nano Lett. 2019 Mar 13;19(3):1827-1831.

Insert

7D12 Gly42 nanobody

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

trc promoter

Available since

May 31, 2019

Availability

Academic Institutions and Nonprofits only

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pFastBac K-Flag M33

Plasmid

#1992

Depositor

Robert Kingston

Article

Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

Insert

M33 (Cbx2 Mouse)

Use

Tags

Flag

Expression

Insect

Mutation

Kozak sequence added before ATG

Promoter

Available since

March 23, 2006

Availability

Academic Institutions and Nonprofits only
pPV454

Plasmid

#42922

Depositor

James Lok

Article

Stoltzfus et al PLoS One. 2012;7(6):e38587. doi: 10.1371

Insert

Ce-age-1 promoter (873 bp)::Ce-age-1 cDNA (3549 bp)::Ce-unc-54 terminator (765 bp) (age-1 Nematode)

Use

Tags

Expression

Worm

Mutation

Promoter

Ce-age-1 promoter (873 bp)

Available since

March 4, 2013

Availability

Academic Institutions and Nonprofits only

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Mesp-1916/-1>nls::Cas9::nls

Plasmid

#59988

Purpose

Mesp driver driving nls::Cas9::nls

Depositor

Lionel Christiaen

Article

Stolfi et al Development. 2014 Nov;141(21):4115-20. d

Insert

nls::Cas9::nls

Use

CRISPR

Tags

Expression

Mutation

Reverted mutations from dCas9 to wiltdtype

Promoter

Ciinte.Mesp -1916/-1

Available since

Nov. 18, 2014

Availability

Academic Institutions and Nonprofits only

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VPCT-SUV39H2-L

Plasmid

#89137

Purpose

mammalian expression of full-length 410 amino acid SUV39H2 histone methyltransferase linked to VP16 activation domain

Depositor

Elizabeth Wilson

Article

Askew et al Mol Cell Endocrinol. 2017 Mar 5;443:42-5

Insert

SUV39H2 (SUV39H2 Human)

Use

Tags

VP16

Expression

Mammalian

Mutation

Promoter

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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