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We narrowed to 16,750 results for: eng

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Showing: 2421 - 2440 of 16750 results

  5. hNTa1-qgRNA-pYJA5

    Plasmid
    #217779
    Purpose
    Non-targeting control 1 qgRNA-pYJA5 plasmid from the T.gonfio library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning
    Depositor
    Adriano Aguzzi
    Publication
    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038
    Insert
    Quadruple sgRNA-expression cassette for nontargeting control for gene activation and epigenetic silencing
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Expression
    Mammalian
    Promoter
    human U6, mouse U6, human H1, human 7SK
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    217779-plasmid-map-sequence-id-466021

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  8. pLA2ScIfmTcIfm

    Plasmid
    #193792
    Purpose
    Cassette 1: Expresses frame-shifted CI under PLLacO-1 promoter, Cassette 2: Expresses frame-shifted CI under PLTetO-1 promoter, ColE1 origin of replication, Ampicillin selection
    Depositor
    Sangram Bagh
    Publication
    Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11
    Insert
    Two copies of frame-shifted CI in opposite orientation, controlled by separate promoters and terminators
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    PLLacO-1 and PLTetO-1
    Available since
    April 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    193792-plasmid-map-sequence-id-390100

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  9. pAAV.hSynap.(cyto)iGlucoSnFR-mRuby2 (AAV9)

    Viral prep
    #164510-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV.hSynap.(cyto)iGlucoSnFR-mRuby2 (#164510). In addition to the viral particles, you will also receive purified pAAV.hSynap.(cyto)iGlucoSnFR-mRuby2 plasmid DNA. Synapsin-driven, cytosolic-targeted iGlucoSnFR-mRuby2
    Depositor
    Loren Looger
    Publication
    Keller et al Cell Rep. 2021 Jun 22;35(12):109284. doi
    Promoter
    Syn
    Tags
    mRuby2
    Available since
    Nov. 29, 2021
    Availability
    Academic Institutions and Nonprofits only

  10. hNTo2-qgRNA-pYJA5

    Plasmid
    #217782
    Purpose
    Non-targeting control 2 qgRNA-pYJA5 plasmid from the T.spiezzo library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning
    Depositor
    Adriano Aguzzi
    Publication
    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038
    Insert
    Quadruple sgRNA-expression cassette for nontargeting control for gene ablation
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Expression
    Mammalian
    Promoter
    human U6, mouse U6, human H1, human 7SK
    Available since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    217782-plasmid-map-sequence-id-466020

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  11. Sctrans-PP

    Pooled library
    #153101
    Purpose
    CRISPR library targets all non-essential and conditionally essential transporter genes in S. cerevisiae
    Depositor
    Irina Borodina
    Publication
    Wang et al Metab Eng. 2021 Jan 16. pii: S1096-7176(
    Expression
    Yeast
    Species
    Saccharomyces cerevisiae
    Use
    CRISPR
    Available since
    Jan. 20, 2021
    Availability
    Academic Institutions and Nonprofits only

  13. MKC89_CCR5(UbC-tEPOR-2A-YFP)

    Plasmid
    #232404
    Purpose
    AAV production plasmid for UbC-tEPOR-2A-YFP vector from Fig. 2 that mediates HDR at CCR5 locus using CCR5-sg3 gRNA. YFP is followed by a BGH polyA. AAV genome is flanked by AAV2 ITRs.
    Depositor
    Kyle Cromer, Matthew Porteus
    Publication
    Luna et al Nat Biomed Eng. 2024 Dec;8(12):1540-1552
    Insert
    Truncated Erythropoietin Receptor (EPOR Human)
    Use
    AAV
    Expression
    Mammalian
    Available since
    Feb. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    232404-plasmid-map-sequence-id-462769

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  14. MKC91_tEPOR(UbC-GFP-BGH)

    Plasmid
    #232406
    Purpose
    AAV production plasmid for tEPOR-UbC-GFP-BGH vector from Fig. 1 that mediates HDR at EPOR locus using EPOR-sg1 gRNA. Repair vector introduces premature stop codon into EPOR locus.
    Depositor
    Kyle Cromer, Matthew Porteus
    Publication
    Luna et al Nat Biomed Eng. 2024 Dec;8(12):1540-1552
    Insert
    Truncated Erythropoietin Receptor (EPOR Human)
    Use
    AAV
    Expression
    Mammalian
    Available since
    Feb. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    232406-plasmid-map-sequence-id-462749

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  15. pAAV-CAG-fDIO-mNeonGreen (AAV1)

    Viral prep
    #99133-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-CAG-fDIO-mNeonGreen (#99133). In addition to the viral particles, you will also receive purified pAAV-CAG-fDIO-mNeonGreen plasmid DNA. CAG-driven, Flp-dependent mNeonGreen expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Viviana Gradinaru
    Publication
    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.
    Promoter
    CAG
    Tags
    mNeonGreen (Flp-dependent)
    Available since
    Jan. 6, 2022
    Availability
    Academic Institutions and Nonprofits only

  16. pCMV-ABE8e-eVRQR-P2A-EGFP (HES1425)

    Plasmid
    #242657
    Purpose
    CMV promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with neVRQR(D10A) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pCMV-ABE8e-SpCas9-VRQR(S55R)-P2A-EGFP
    Use
    CRISPR
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    ABE8e mutations in TadA, eVRQR mutations in SpCas…
    Promoter
    CMV and T7
    Available since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242657-plasmid-map-sequence-id-488990

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  17. pAAV-ihSyn1-DIO-tTA (AAV1)

    Viral prep
    #99121-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-ihSyn1-DIO-tTA (#99121). In addition to the viral particles, you will also receive purified pAAV-ihSyn1-DIO-tTA plasmid DNA. Cre-dependent expression of the tet-off transactivator from an inducible synapsin promoter. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Viviana Gradinaru
    Publication
    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.
    Promoter
    ihSyn1
    Available since
    April 4, 2019
    Availability
    Academic Institutions and Nonprofits only

  18. MKC86_HBA1(tEPOR-2A-YFP)

    Plasmid
    #232405
    Purpose
    AAV production plasmid for tEPOR-2A-YFP vector from Fig. 2 that mediates HDR at HBA1 locus using HBA1-sg4 gRNA. HBA1 UTRs flank full tEPOR-2A-YFP cassette. Homology arms mediate full HBA1 replacement.
    Depositor
    Kyle Cromer, Matthew Porteus
    Publication
    Luna et al Nat Biomed Eng. 2024 Dec;8(12):1540-1552
    Inserts
    Truncated Erythropoietin Receptor (EPOR Human)
    Hemoglobin Subunit Alpha 1 (HBA1 Human)
    Use
    AAV
    Expression
    Mammalian
    Available since
    April 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    232405-plasmid-map-sequence-id-462759

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  20. hNTa2-qgRNA-pYJA5

    Plasmid
    #217780
    Purpose
    Non-targeting control 2 qgRNA-pYJA5 plasmid from the T.gonfio library; it can be used as a ready-to-go control and provides the three constant regions for the three-fragment PCRs for qgRNA cloning
    Depositor
    Adriano Aguzzi
    Publication
    Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038
    Insert
    Quadruple sgRNA-expression cassette for nontargeting control for gene activation and epigenetic silencing
    Use
    CRISPR, Lentiviral, and Synthetic Biology
    Expression
    Mammalian
    Promoter
    human U6, mouse U6, human H1, human 7SK
    Available since
    April 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    217780-plasmid-map-sequence-id-469426

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Showing: 2421 - 2440 of 16750 results