We narrowed to 3,135 results for: actin

1. PTTG-bio-His

   Plasmid

   #51822

   Purpose

   Expresses full-length Pituitary tumor-transforming gene 1 protein-interacting protein ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   PTTG (PTTG1IP Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. PHB2 S39A-mCherry

   Plasmid

   #232786

   Purpose

   Encodes an mCherry-tagged Prohibitin2/PHB2 non-phosphorylatable on Ser39

   Depositor

   Giulia Bertolin

   Article

   Bertolin et al Life Sci Alliance. 2021 Apr 5;4(6):e2020

   Insert

   PHB2 (PHB2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Changed Ser 39 to Ala

   Promoter

   CMV

   Available since

   March 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PHB2 S39D-mCherry

   Plasmid

   #232787

   Purpose

   Encodes an mCherry-tagged Prohibitin2/PHB2 constitutively phosphorylated on Ser39

   Depositor

   Giulia Bertolin

   Article

   Bertolin et al Life Sci Alliance. 2021 Apr 5;4(6):e2020

   Insert

   PHB2 (PHB2 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Changed Serine 39 to Aspartate

   Promoter

   CMV

   Available since

   March 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. tetO-NFYB

   Plasmid

   #170697

   Purpose

   doxycycline-inducible overexpression of NFYB

   Depositor

   Paul Burridge

   Article

   Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

   Insert

   NYFB (NFYB Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE promoter, Tet-ON

   Available since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Linked VLP NES

   Plasmid

   #182432

   Purpose

   Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20-AcNES1 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al bioRxiv 2022.11.24.517869

   Inserts

   VP2C-FPPS-NES
   deltaVP1
   

   Use

   Synthetic Biology; Metabolic engineering

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1 and GAL10

   Available since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Linked VLP NES (M)

   Plasmid

   #182434

   Purpose

   YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20(F96W-N127W)-AcNES1 (GAL10 promoter). Contains uracil marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al bioRxiv 2022.11.24.517869

   Inserts

   VP2C-FPPS(M)-NES
   deltaVP1
   

   Use

   Synthetic Biology; Metabolic engineering

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1 and GAL10

   Available since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Linked VLP NES (G)

   Plasmid

   #182476

   Purpose

   YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-ERG20(F96C)-AcNES1 (GAL10 promoter). Contains uracil marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al bioRxiv 2022.11.24.517869

   Inserts

   VP2C-FPPS(G)-NES
   deltaVP1
   

   Use

   Synthetic Biology; Metabolic engineering

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1 and GAL10

   Available since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCu-His6-Atg13[571–700]-Ss(424)

   Plasmid

   #166852

   Purpose

   Expresses 6xHis tagged ATG13 [571–700] fragment with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast.

   Depositor

   Daniel Klionsky

   Article

   Gatica et al Autophagy. 2020 Jun;16(6):1007-1020. doi

   Insert

   Atg13[571–700] (ATG13 Budding Yeast)
   

   Use

   Tags

   6xHis

   Expression

   Yeast

   Mutation

   Promoter

   pCu

   Available since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBADHISTEV_PfeEH258A

   Plasmid

   #129122

   Purpose

   expresses H258A mutant of PfeE in E.coli

   Depositor

   James Naismith

   Article

   Perraud et al ACS Chem Biol. 2018 Sep 21;13(9):2603-26

   Insert

   Periplasmic Esterase PfeE (PA2689 Pseudomonas aeruginosa PAO1)
   

   Use

   Tags

   TEV protease cleavable 6xHis

   Expression

   Bacterial

   Mutation

   Signal peptide sequence has been removed, mutat…

   Promoter

   PBAD

   Available since

   Aug. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBADHISTEV_PfeES157A

    Plasmid

    #129121

    Purpose

    expresses S157A mutant of PfeE in E.coli

    Depositor

    James Naismith

    Article

    Perraud et al ACS Chem Biol. 2018 Sep 21;13(9):2603-26

    Insert

    Periplasmic Esterase PfeE (PA2689 Pseudomonas aeruginosa PAO1)
    

    Use

    Tags

    TEV protease cleavable 6xHis

    Expression

    Bacterial

    Mutation

    Signal peptide sequence has been removed, mutat…

    Promoter

    PBAD

    Available since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. p3 FLAG_CMV14/OCTN2 (delta14_22/delta447_454)

    Plasmid

    #112802

    Purpose

    Expression of OCTN2 mutant

    Depositor

    Katarzyna Nalęcz

    Article

    Czeredys et al PLoS One. 2013 Dec 13;8(12):e82105. doi:

    Insert

    OCTN2/SLC22A5 (Slc22a5 Rat)
    

    Use

    Tags

    3 x Flag

    Expression

    Mammalian

    Mutation

    amino acids 14_22 and 447_454 deleted

    Promoter

    Available since

    Aug. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCI-neo-(HA)3-ubiquitin

    Plasmid

    #196991

    Purpose

    Expresses HA-tagged ubiquitin in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Mattera et al J Biol Chem. 2006 Mar 10;281(10):6874-83

    Insert

    ubiquitin (UBC Human)
    

    Use

    Tags

    triple HA tag

    Expression

    Mammalian

    Mutation

    silent mutation in ubiquitin codon 13

    Promoter

    CMV

    Available since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Human Beta-catenin GFP

    Plasmid

    #71367

    Purpose

    mammalian expression of human Beta-catenin GFP

    Depositor

    Alpha Yap

    Article

    Han et al J Biol Chem. 2014 Mar 14;289(11):7764-75

    Insert

    Beta-catenin (CTNNB1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. HA-EglN1-pcDNA3

    Plasmid

    #18963

    Depositor

    William Kaelin

    Article

    Ivan et al Proc Natl Acad Sci U S A. 2002 Oct 15. 9

    Insert

    Egg laying Nine - 1 (EGLN1 Human)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    wt cDNA

    Promoter

    Available since

    Aug. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCS2 LRP6-eGFP

    Plasmid

    #180143

    Purpose

    to make movies of LRP6 vesicles

    Depositor

    Edward De Robertis

    Article

    Colozza et al Sci Rep. 2020 Dec 9;10(1):21555. doi: 10

    Insert

    Human LRP6 (LRP6 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. tetO-FOSL1

    Plasmid

    #170687

    Purpose

    doxycycline-inducible overexpression of FOSL1

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    FOSL1 (FOSL1 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet-ON

    Available since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GFP-ataxin-3

    Plasmid

    #89975

    Purpose

    Expresses GFP-tagged ataxin-3

    Depositor

    Nico Dantuma

    Article

    Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

    Insert

    ataxin-3 (ATXN3 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRK6-HA-TAK1

    Plasmid

    #44160

    Depositor

    Xin Lin

    Article

    Blonska et al J Biol Chem. 2005 Dec 30;280(52):43056-6

    Insert

    Tak1 (Map3k7 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

19. His-ZZ-TEV-SNAPf-BICDR1

    Plasmid

    #111858

    Purpose

    N-terminal SNAPf tag on BICDR1 for expression in Sf9 cells. 8xHis-ZZ-SNAPf N-terminal tag, TEV site to cleave 8xHis-ZZ.

    Depositor

    Andrew Carter

    Article

    Urnavicius et al Nature. 2018 Feb 7;554(7691):202-206. do

    Insert

    BICDR1 (Bicdl1 Mouse)
    

    Use

    Tags

    His-tag, SNAPf-tag, TEV site, and ZZ

    Expression

    Insect

    Mutation

    R250Q, Optimised for Sf9 expression

    Promoter

    Available since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCS2-LRP6-3XFlag

    Plasmid

    #180144

    Purpose

    flag tagged LRP6

    Depositor

    Edward De Robertis

    Article

    Colozza et al Sci Rep. 2020 Dec 9;10(1):21555. doi: 10

    Insert

    Human LRP6 (LRP6 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    SP6

    Available since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge