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We narrowed to 2,488 results for: PORA

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Showing: 2441 - 2460 of 2488 results

  1. pRCA803 - pBA904 Puro-T2A-GFP KLF5 g1 CRISPRa guide (pRCA360 backbone) 666

    Plasmid
    #238172
    Purpose
    Lentiviral CRISPR guide vector expressing a KLF5 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture
    Depositor
    Thomas Norman
    Article
    Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415
    Insert
    KLF5 sgRNA CRISPRa (KLF5 Synthetic, Human)
    Use
    CRISPR and Lentiviral
    Tags
    GFP
    Available Since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    238172-plasmid-map-sequence-id-474457

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  2. pRCA804 - pBA904 Puro-T2A-GFP KLF5 g2 CRISPRa guide (pRCA360 backbone) 665

    Plasmid
    #238173
    Purpose
    Lentiviral CRISPR guide vector expressing a KLF5 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture
    Depositor
    Thomas Norman
    Article
    Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415
    Insert
    KLF5 sgRNA CRISPRa (KLF5 Synthetic, Human)
    Use
    CRISPR and Lentiviral
    Tags
    GFP
    Available Since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    238173-plasmid-map-sequence-id-474456

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  3. pRCA895 - pBA904 Puro-T2A-BFP KLF4 g1 CRISPRa guide guide (pRCA594 backbone) 668

    Plasmid
    #238187
    Purpose
    Lentiviral CRISPR guide vector expressing a KLF4 sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture
    Depositor
    Thomas Norman
    Article
    Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415
    Insert
    KLF4 sgRNA CRISPRa (KLF4 Synthetic, Human)
    Use
    CRISPR and Lentiviral
    Tags
    mTagBFP2
    Available Since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    238187-plasmid-map-sequence-id-474450

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  5. pCJ205

    Plasmid
    #162676
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating C224A an
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    C224A, C529A; codon optimized for expression in E…
    Promoter
    T7/lac
    Available Since
    Feb. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    162676-plasmid-map-sequence-id-320828

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  6. pCJ198

    Plasmid
    #162671
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating F495I mutation.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    F495I; codon optimized for expression in E. coli …
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162671-plasmid-map-sequence-id-321447

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  7. pCJ196

    Plasmid
    #162669
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating catalytic mutation, S225A
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    S225A; codon optimized for expression in E. coli …
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162669-plasmid-map-sequence-id-321445

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  8. pCJ204

    Plasmid
    #162677
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating C224H and C529F mutations.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    C224H, C529F; codon optimized for expression in E…
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162677-plasmid-map-sequence-id-321439

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  9. pCJ201

    Plasmid
    #162674
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating C224W and C529SS mutations.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1)
    Tags
    His
    Expression
    Bacterial
    Mutation
    C224W, C529SS; codon optimized for expression in …
    Promoter
    T7/lac
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    162674-plasmid-map-sequence-id-320830

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  10. EF1a-L274GMmMetRS-T2A-mCherry

    Plasmid
    #220800
    Purpose
    Lentivirus expressing mutant tRNA synthetase for incorporation of noncanonical amino acids in nascent proteins plus mCherry reporter and puro resistance
    Depositor
    Elizabeth Kirby
    Article
    Sridharan et al Stem Cell Res Ther. 2024 Jun 26;15(1):18
    Insert
    Mars1 (Mars1 Mouse)
    Use
    Lentiviral
    Tags
    2xFLAG and T2A-mCherry
    Mutation
    mutation in MetRS to change aa 274 from L to G
    Promoter
    EF1a
    Available Since
    June 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    220800-plasmid-map-sequence-id-437285

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  11. pCJ217

    Plasmid
    #162685
    Purpose
    pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating two point mutations, G489C and S530C, to introduce a 6th disulfide bond (from PETase).
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), with mutations to introduce a 6th disulfide bond from PETase
    Tags
    His
    Expression
    Bacterial
    Mutation
    G489C, S530C; codon optimized for E. coli K12
    Promoter
    T7/lac
    Available Since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    162685-plasmid-map-sequence-id-320886

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  12. pPaGE Pyl TAG FliC T248TAG

    Plasmid
    #160089
    Purpose
    Encodes for MmPyl tRNA synthetase/tRNA pair and for flagellin protein (FliC) with TAG mutation at site 248, for unnatural amino acid incorporation into the flagellin protein in Pseudomonas aeruginosa.
    Depositor
    Lital Alfonta
    Article
    Ozer et al Sci Adv. 2021 Jun 11;7(24). pii: 7/24/ea
    Inserts
    Pyrrolysyl tRNA(cua) (Methanosarcina mazei)
    Pyrrolysyl tRNA synthetase (Methanosarcina mazei)
    Flagellin
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    Changed Threonine 248 to TAG stop-codon.
    Promoter
    Pseudomonas aeruginosa Leu-tRNA native promoter a…
    Available Since
    June 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    160089-plasmid-map-sequence-id-314779

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  13. pcDNA5 FRT/TO SF-HERC2 C4762S (ShB-R)

    Plasmid
    #55614
    Purpose
    Dox-inducible expression of full length, ShB-resistant, 3xFLAG/STREP tagged C4762S (catalytically inactive) HERC2 in Flp-In T-Rex cells
    Depositor
    David Chan
    Article
    Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.
    Insert
    3xFLAG tagged HERC2 (C4762S) (HERC2 Human)
    Tags
    3xFLAG and STREP
    Expression
    Mammalian
    Mutation
    C4762 mutated to serine to render HERC2 catalytic…
    Available Since
    Sept. 25, 2014
    Availability
    Academic Institutions and Nonprofits only
    55614-plasmid-map-sequence-id-86500

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  14. pCJ208

    Plasmid
    #162681
    Purpose
    Lidded variant of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) removing a seven-residue loop of PETase (Trp185:Phe191, PETase numbering) and replacing it with Gly251:Thr472 from MHETase; codon optimized for expression in E. coli K12, with C-terminal His tag.
    Depositor
    Gregg Beckham
    Article
    Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11
    Insert
    PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) incorporating the MHETase lid
    Tags
    His
    Expression
    Bacterial
    Mutation
    Replacement of W185-F191 with G251-T472 from MHET…
    Promoter
    T7/lac
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    162681-plasmid-map-sequence-id-321444

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  15. XLone-Puro beta-catenin P2A eGFP-NLS

    Plasmid
    #155189
    Purpose
    PiggybacTransposon-based tunable and temporal expression control of mutated beta-catenin and nuclear eGFP
    Depositor
    Xiaoping Bao
    Article
    Chang et al Biomaterials. 2022 May 6;285:121569. doi
    Insert
    beta-catenin (Ctnnb1 Mouse)
    Use
    Unspecified; Piggybac transposon
    Tags
    P2A eGFP NLS
    Expression
    Mammalian
    Mutation
    Sites phosphorylated by GSK-3beta have been mutat…
    Promoter
    TRE3G
    Available Since
    June 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    155189-plasmid-map-sequence-id-307963

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  16. Desmoplakin I affinity mutant (F40-based tension sensor)

    Plasmid
    #119190
    Purpose
    The affinity mutant for the F40-based human desmoplakin I tension sensor incorporates a point mutation that exhibits enhanced keratin association.
    Depositor
    Alex Dunn
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin I(S2849G)-[mTFP1-F40-mEYFP] (internal-1945) (DSP Human)
    Use
    Transposon
    Tags
    mTFP1-F40-mEYFP
    Expression
    Mammalian
    Mutation
    inserted F40-based tension sensor module after aa…
    Promoter
    TCE
    Available Since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    119190-plasmid-map-sequence-id-232293

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Showing: 2441 - 2460 of 2488 results