We narrowed to 16,127 results for: Por

1. UAS-u(S)Cas9

   Plasmid

   #127383

   Purpose

   Expresses Cas9 at high level

   Depositor

   Michael Boutros

   Article

   Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

   Insert

   uORF(S)-Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   UAST

   Available since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. mCherry Codon 59 sgRNA

   Plasmid

   #109431

   Purpose

   MLM3636 backbone containing a gRNA that guides Cas9 to codon 59 of mCherry in the ACE reporter

   Depositor

   Reuben Harris

   Article

   St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

   Insert

   mCherry codon 59 gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET21-GFP-AURKA

   Plasmid

   #99875

   Purpose

   Expression of GFP-AURKA for protein purification in bacteria

   Depositor

   Marc Tramier

   Article

   Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Use

   Tags

   GFP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTriEx-mCherry-LINXa3

   Plasmid

   #73614

   Purpose

   Light-induced nuclear export switch for microscopy in mammalian cells.

   Depositor

   Brian Kuhlman

   Article

   Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

   Insert

   LINXa3
   

   Use

   Tags

   mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Chicken B-Actin

   Available since

   April 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-ZIKVA-GFP-puro

   Plasmid

   #140090

   Purpose

   Fluorescence activatable reporter of ZIKV NS2B-NS3 protease activity

   Depositor

   Jorge Luis Arias-Arias

   Article

   Arias-Arias et al J Biol Chem. 2020 Jan 9. pii: RA119.0113

   Insert

   ZIKVA-GFP
   

   Use

   Lentiviral; Low expression

   Tags

   6XHis tag and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTriEx-mCherry-LINXa3-nano

   Plasmid

   #73616

   Purpose

   Light-induced nuclear export switch fusion to nano for microscopy in mammalian cells.

   Depositor

   Brian Kuhlman

   Article

   Yumerefendi et al Nat Chem Biol. 2016 Jun;12(6):399-401. d

   Insert

   LINXa3-nano
   

   Use

   Tags

   mCherry

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   Available since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-DENV2A-GFP-puro

   Plasmid

   #140089

   Purpose

   Fluorescence activatable reporter of DENV-2 NS2B-NS3 protease activity

   Depositor

   Jorge Luis Arias-Arias

   Article

   Arias-Arias et al J Biol Chem. 2020 Jan 9. pii: RA119.0113

   Insert

   DENV2A-GFP
   

   Use

   Lentiviral; Low expression

   Tags

   6XHis tag and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKrox24(2xD-E)DsRed

   Plasmid

   #200111

   Purpose

   Fluorescent reporter for in cell monitoring of receptor tyrosine kinase-MAP ERK pathway activity

   Depositor

   Pavel Krejčí

   Article

   Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

   Insert

   modified EGR1 promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   highly modified sequence

   Promoter

   2 copies of D-E element, no other promoter elemen…

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. SEP-Nanoluc-cyOFP1

   Plasmid

   #141082

   Purpose

   prototype of the bioluminescent reporter pHLuc, for detecting extracellular pH changes in tumor acidosis

   Depositor

   Jeak Ling Ding

   Article

   Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

   Insert

   SEP-Nanoluc-cyOFP1
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Deletion of amino acids DISGG from positions 673 …

   Promoter

   EF1α

   Available since

   June 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET21-GFP-AURKA-mCherry

    Plasmid

    #99876

    Purpose

    Expression of GFP-AURKA-mCherry for protein purification in bacteria

    Depositor

    Marc Tramier

    Article

    Bertolin et al Nat Commun. 2016 Sep 14;7:12674. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Use

    Tags

    GFP and mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-C1-KSENB-Nup133-mut

    Plasmid

    #87339

    Purpose

    To express human Nup133 in mammalian cells. It contains synonymous mutations that are not targeted by shRNA

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    NUP133 (NUP133 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    shRNA resistant changes; deletion of Nup133 AA1-7

    Promoter

    CMV

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAS_4xG1 PylT sfGFP 150TAG

    Plasmid

    #154767

    Purpose

    plasmid with 4xG1 PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    150TAG

    Promoter

    EF1

    Available since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL2-MCT2Prom0.6

    Plasmid

    #87353

    Purpose

    Human monocarboxylate transporter 2 (MCT2) promoter 0.6 kbp

    Depositor

    Saroj Mathupala

    Article

    Caruso et al Neoplasia. 2017 Jan 13;19(2):121-134. do

    Insert

    MCT2 (SLC16A7 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    0.6 kbp proximal promoter of human MCT2 (nested d…

    Available since

    March 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pB80-SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)

    Plasmid

    #174642

    Purpose

    Optogenetic coupling to tetramerized moss kinesin-14 via SSPB(micro) for highly efficient retrograde transport

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)
    

    Use

    Tags

    SSPB(micro)-Venus

    Expression

    Mammalian

    Mutation

    SSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin1…

    Promoter

    Chicken beta-actin

    Available since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET15b-ITPA

    Plasmid

    #60824

    Purpose

    to purify human ITPA from bacteria

    Depositor

    Youri Pavlov

    Article

    Porta et al Acta Crystallogr Sect F Struct Biol Crys

    Insert

    ITPA (ITPA Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGM55

    Plasmid

    #71249

    Purpose

    luciferase reporter with hGM-CSF -55 to +28 minimal promoter

    Depositor

    Peter Cockerill

    Article

    Bert et al Plasmid. 2000 Sep;44(2):173-82.

    Insert

    GM-CSF minimal promoter (-55 to +28) (CSF2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    GM-CSF minimal

    Available since

    Dec. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAS_4xhyb PylT sfGFP 150TAG

    Plasmid

    #154771

    Purpose

    plasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid ) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    150TAG

    Promoter

    EF1

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pB80-SSPB(micro)-GFP-ppKin14VIb(861-1321)

    Plasmid

    #174639

    Purpose

    Optogenetic coupling to dimeric moss kinesin-14 for retrograde transport via SSPB(micro)

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    SSPB(micro)-GFP-ppKinVIb(861-1321)
    

    Use

    Tags

    SSPB(micro)-GFP

    Expression

    Mammalian

    Mutation

    SSPB:Arg73Gln; EGFP: Met1Del; ppKin14VIb(aa861-13…

    Promoter

    Chicken beta-actin

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pOPTXcGMPRELUC

    Plasmid

    #68503

    Purpose

    cyclic nucleotide reporter for bacteria or plant cells

    Depositor

    Helen Irving

    Article

    Wheeler et al BMC Biotechnol. 2013 Nov 9;13:97. doi: 1

    Insert

    OPTX promoter with cGMPRE (AT1G33440 Mustard Weed)
    

    Use

    Tags

    luciferase

    Expression

    Bacterial and Plant

    Mutation

    3x cGMPRE inserted 190bp 5' of ATG (cGMPRE =…

    Promoter

    OPTX promoter with cGMPRE

    Available since

    Sept. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pRP_8xgRNA-FLuc

    Plasmid

    #135937

    Purpose

    Firefly luciferase reporter containing 8 copies of a gRNA binding site for light-inducible dCas9 activation.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    8 copies of gRNA binding site (5'-AAAGGTCGAGAAACTGCAAA-3')
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    minCMV

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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