We narrowed to 11,528 results for: Vars;

1. pECFP-DEVDR-Venus

   Plasmid

   #24537

   Depositor

   Peter Sorger

   Article

   Albeck et al PLoS Biol. 2008 Dec 2. 6(12):2831-52.

   Insert

   ECFP-DEVDR-Venus
   

   Use

   Tags

   ECFP and Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 11, 2010

   Availability

   Academic Institutions and Nonprofits only

2. pX330A_dCas9-1x7

   Plasmid

   #63601

   Purpose

   Expresses dCas9 and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

   Insert

   humanized S. pyogenes dCas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available Since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. TCF4 in pENTR

   Plasmid

   #216569

   Purpose

   gateway cloning donor vector containing TCF4

   Depositor

   Christopher Loewen, Douglas Allan

   Insert

   TCF4 (TCF4 Human)
   

   Use

   Gateway entry plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTW211b5

   Plasmid

   #136927

   Purpose

   Directed evolution of SpCas9

   Depositor

   David Liu

   Article

   Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

   Insert

   Cas9(N)-NpuN
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   see manuscript

   Promoter

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. 5HT2A-APEX2

   Plasmid

   #215558

   Purpose

   Stable, inducible expression of 5HT2A

   Depositor

   Nevan Krogan

   Article

   Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

   Insert

   Flag tag-5HT2A(1-452)-APEX2-5HT2A (453-471)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. mEGFP-30nmSAH-DmrA

   Plasmid

   #61680

   Purpose

   GFP-labeled SAH scaffold with DmrA attachment site

   Depositor

   Kristen Verhey

   Article

   Norris et al J Cell Biol. 2014 Nov 10;207(3):393-406.

   Insert

   mEGFP-30nmSAH-DmrA
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. epB_UbC_CymRV5-nls-Halox2_DEx4

   Plasmid

   #119907

   Purpose

   ePiggyBac mammalian expression plasmid for CymR-Halo fusion

   Depositor

   Orion Weiner

   Article

   Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

   Insert

   CymRV5-nls-Halox2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5/FRT/TO_HaloTag9_SKL

   Plasmid

   #175542

   Purpose

   Peroxysomal expression of HaloTag9 in mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   HaloTag9-SKL
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Promoter

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mTFP1-C1

   Plasmid

   #54613

   Purpose

   Localization: C1 Cloning Vector, Excitation: 462, Emission: 492

   Depositor

   Robert Campbell, Michael Davidson

   Article

   Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

   Type

   Empty backbone

   Use

   Tags

   mTFP1

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCA_e-1

    Plasmid

    #202096

    Purpose

    Receiver cloning vector for even-1 level constructs. Contains lacZ for blue/white screening.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available Since

    Aug. 23, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pL0R_lacZ

    Plasmid

    #202095

    Purpose

    Receiver cloning vector for making level 0 parts. Contains lacZ for blue/white screening.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available Since

    Aug. 23, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pCA_o-1

    Plasmid

    #202091

    Purpose

    Receiver cloning vector for odd-1 level constructs. Contains lacZ for blue/white screening.

    Depositor

    Christopher Dupont

    Article

    Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    None

    Promoter

    Available Since

    Aug. 23, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pAAV-hSyn-GluA2-scFv-mNeon-KDEL

    Plasmid

    #230057

    Purpose

    AAV plasmid that expresses a a single chain variable fragment (scFv) for anti- GluA2 with a C-terminal mNeon. Encodes an ER retrieval motif (KDEL) at C-terminus of mNeon.

    Depositor

    Matthew Kennedy

    Insert

    Anti-GluA2 single chain variable fragment (scFv)
    

    Use

    AAV

    Tags

    ER retrieval motif (KDEL) and mNeon

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pST-K

    Plasmid

    #44560

    Purpose

    for ectopic constitutive expression of mycobacterial proteins

    Depositor

    Vinay Nandicoori

    Article

    Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

    Type

    Empty backbone

    Use

    E.coli-mycobacteria shuttle vector

    Tags

    Flag tag and His tag

    Expression

    Bacterial

    Mutation

    Promoter

    UV15 promoter

    Available Since

    Oct. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-oChIEF-citrine

    Plasmid

    #50974

    Purpose

    AAV transfer vector with mammalian codon-optimized ChIEF-citrine between AAV2 ITR

    Depositor

    Roger Tsien

    Article

    Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

    Insert

    ChIEF-citrine
    

    Use

    AAV

    Tags

    citrine

    Expression

    Mutation

    ChIEF gene is mammalian codon optimized

    Promoter

    human synapsin promoter

    Available Since

    Feb. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. mTagRFP-T-Endosomes-14

    Plasmid

    #58014

    Purpose

    Localization: Endosomes, Excitation: 555, Emission: 584

    Depositor

    Michael Davidson

    Article

    Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

    Insert

    Endosomes (RHOB Human)
    

    Use

    Tags

    mTagRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 31, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

17. pTiger-OptoSOS

    Plasmid

    #86439

    Purpose

    Single-construct blue light optogenetic Ras activator for use in Drosophila

    Depositor

    Jared Toettcher

    Article

    Johnson et al Dev Cell. 2017 Jan 23;40(2):185-192. doi

    Insert

    OptoSOS
    

    Use

    Tags

    tagRFP

    Expression

    Insect

    Mutation

    Promoter

    Gal4-UAS

    Available Since

    Feb. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pST-KiT

    Plasmid

    #44562

    Purpose

    for integrative inducible expression of mycobacterial proteins

    Depositor

    Vinay Nandicoori

    Article

    Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

    Type

    Empty backbone

    Use

    E.coli-mycobacteria shuttle vector

    Tags

    Flag tag and His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pX165-LZ3 Cas9

    Plasmid

    #140561

    Purpose

    Expresses LZ3 Cas9 in mammalian cells

    Depositor

    Feng Zhang

    Article

    Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mutation

    N690C T769I G915M N980K

    Promoter

    Available Since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. hSgk1_v2 Flag

    Plasmid

    #86040

    Purpose

    full length human Sgk1_i2 with Flag tag

    Depositor

    Christie Thomas

    Article

    Raikwar et al Am J Physiol Renal Physiol. 2008 Nov;295

    Insert

    Alternate transcript v2 of human Sgk1 (SGK1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    v2

    Promoter

    CMV

    Available Since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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