Addgene

We narrowed to 3,037 results for: ALC

Showing: 2461 - 2480 of 3037 results

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RabV CVS-N2c(deltaG)-GCaMP6f

Plasmid

#73466

Purpose

Expresses GCaMP6f for calcium imaging

Depositor

Thomas Jessell

Article

Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

Insert

GCaMP6f

Use

Neurotropic virus

Tags

Expression

Mutation

Promoter

Available since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV2-GCaMP6f-P2A-Vmn1r78

Plasmid

#133413

Purpose

Fluorescent calcium reporter and mouse vomeronasal 1 receptor 78

Depositor

Tim Holy

Article

Lee et al Science. 2019 Dec 13;366(6471):1384-1389

Insert

GCaMP6f-P2A-vmn1r78 (Vmn1r78 Mouse)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV2-GCaMP6f-P2A-Vmn1r86

Plasmid

#133415

Purpose

Fluorescent calcium reporter and mouse vomeronasal 1 receptor 86

Depositor

Tim Holy

Article

Lee et al Science. 2019 Dec 13;366(6471):1384-1389

Insert

GCaMP6f-P2A-vmn1r86 (Vmn1r86 Mouse)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV2-GCaMP5g-P2A-Vmn1r58

Plasmid

#133418

Purpose

Fluorescent calcium reporter and mouse vomeronasal 1 receptor 58

Depositor

Tim Holy

Article

Lee et al Science. 2019 Dec 13;366(6471):1384-1389

Insert

GCaMP5g-P2A-PAmCherry-Vmn1r58 (Vmn1r58 Mouse)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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MBP-His6-TEV-MMS2

Plasmid

#25465

Depositor

Cynthia Wolberger

Article

Eddins et al Nat Struct Mol Biol. 2006 Oct . 13(10):9

Insert

MMS2 (MMS2, UBC13 Budding Yeast)

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

Available since

July 21, 2010

Availability

Academic Institutions and Nonprofits only
pUF-AsCpf1-pre-gRNA

Plasmid

#137849

Purpose

AsCpf1 and gRNA expression plasmid for P. falciparum with yDHODH selectable marker.

Depositor

Josh Beck

Article

Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

Insert

AsCpf1 and pre-gRNA cassette

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 7, 2020

Availability

Academic Institutions and Nonprofits only

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pYFAC-CH1

Plasmid

#168977

Purpose

AMA1 based expression in filamentous fungi. pYFAC-pyrG carrying PalcA ; unique restriction site: PacI

Depositor

Yit Heng Chooi

Article

Hu et al Chem Sci. 2018 Nov 22;10(5):1457-1465. d

Type

Empty backbone

Use

Synthetic Biology; Heterologous expression in fil…

Tags

Expression

Mutation

Promoter

Available since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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Fast-GCaMP-EF05

Plasmid

#52558

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

Insert

Fast-GCaMP-EF05

Use

Tags

Expression

Insect

Mutation

changed from GCaMP3: D397N/G398A/N399D

Promoter

Available since

April 15, 2014

Availability

Academic Institutions and Nonprofits only
CaYin1 NES

Plasmid

#33318

Depositor

Robert Campbell

Article

Ding et al Anal Chem. 2011 Dec 15;83(24):9687-93. d

Insert

CaYin1 NES

Use

Tags

NES sequence LALKLAGLDIGS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pRSFDuet1_CoiA1_CoiB

Plasmid

#208761

Purpose

Precursor peptide CoiA1 and synthase CoiB, for the co-expression of CoiA1 in E.coli.

Depositor

Wilfred van der Donk

Article

Luo et al Anal Chem. 2024 Jan 30;96(4):1767-1773.

Inserts

CoiA1
CoiB

Use

Tags

Hisx6 Tag and SUMO

Expression

Bacterial

Mutation

Changed Ser 20 to Asn, Changed Arg 84 to Lys, bot…

Promoter

T7

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-fCAMK-GFP

Plasmid

#22917

Depositor

Edward Callaway

Article

Nathanson et al Front Neural Circuits. 2009 . 3():19.

Insert

fugu α-calcium/calmodulin-dependent protein kinase II promoter driving GFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2010

Availability

Academic Institutions and Nonprofits only

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pfTRAP(VWA)

Plasmid

#50049

Purpose

Expresses plasmodium falciparum TRAP VWA domain (41-240) in mammalian cells

Depositor

Timothy Springer

Article

Song et al Proc Natl Acad Sci U S A. 2012 Dec 26;10

Insert

pfTRAP(vwa) (TRAP Synthetic)

Use

Tags

6Xhis tag

Expression

Mammalian

Mutation

C55G, N132S

Promoter

CMV

Available since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only
pHAGE-eGFP-TAX1BP1 A114Q

Plasmid

#175767

Purpose

Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 A114Q

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

TAX1BP1 (TAX1BP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

A114Q

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pIhh-5A9-LUC

Plasmid

#46318

Depositor

Xiangli Yang

Article

Wang et al Development. 2009 Dec;136(24):4143-53. d

Insert

5 copies of A9 sequence from Ihh promoter

Use

Tags

luciferase

Expression

Mammalian

Mutation

Promoter

mouse osteocalcin gene 2 promoter

Available since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only
pUC19/Tp-mChe

Plasmid

#183165

Purpose

Construct for transient expression of tonoplast-targeted mCherry in plants.

Depositor

Qinlong Zhu, Yao-Guang Liu

Article

Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

Insert

Tp-mCherry

Use

Tags

28 aa of A. thaliana CALCINEURIN B-LIKE PROTEIN 2…

Expression

Plant

Mutation

Promoter

Available since

April 26, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-eGFP-TAX1BP1 CC3D

Plasmid

#175776

Purpose

Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 CC3D

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

TAX1BP1 (TAX1BP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

CC3D

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-eGFP-TAX1BP1 CC2D

Plasmid

#175775

Purpose

Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 CC2D

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

TAX1BP1 (TAX1BP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

CC2D

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-eGFP-TAX1BP1 CC1D

Plasmid

#175774

Purpose

Lentiviral vector expressing N-terminally tagged eGFP-TAX1BP1 CC1D

Depositor

Wade Harper

Article

Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

Insert

TAX1BP1 (TAX1BP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

CC1D

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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Fast-GCaMP-RS15

Plasmid

#52652

Purpose

genetically encodable fluorescent calcium indicator protein to monitor neuronal activity

Depositor

Samuel Wang

Article

Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.

Insert

Fast-GCaMP-RS15

Use

Tags

Expression

Mammalian

Mutation

deleted G47/H48 from GCaMP3

Promoter

T7

Available since

May 6, 2014

Availability

Academic Institutions and Nonprofits only
pSG5-FLAG-CaMKKbeta rat 1-460

Plasmid

#33324

Depositor

Anthony Means

Article

Green et al Cell Signal. 2011 Dec;23(12):2005-12. Ep

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)

Use

Tags

FLAG

Expression

Mammalian

Mutation

truncated at 460; S3V, G96R, and P108L also prese…

Promoter

SV40

Available since

April 17, 2012

Availability

Academic Institutions and Nonprofits only