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pKF-D2irTNP2AG-T2APuroR-5kwh

Plasmid

#128255

Purpose

Mammalian linearizer gene circuit based on negative feedback in a Flp-In expression system controlling the PuroR gene.

Depositor

Gabor Balazsi

Article

Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:

Insert

hTetR::P2A::EGFP::T2A::PuroR

Use

Synthetic Biology; Flp-in expression vector

Expression

Mammalian

Promoter

CMV-D2ir promoter

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_NCK1-2/3

Plasmid

#91420

Purpose

Protein expression and purification of human SH3 domain construct NCK1-2/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

NCK1-2/3 (NCK1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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GPD 104QDProGFP p416

Plasmid

#15569

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 104Q Delta Pro (HTT Human)

Tags

GFP

Expression

Yeast

Mutation

Proline-rich region removed

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pCDNA_GFP_mTLN1-FL-LBD

Plasmid

#164839

Purpose

Expression of GFP tagged FL mouse talin1 mutant

Depositor

Tina Izard

Article

Rangarajan et al J Biol Chem. 2020 Sep 11;295(37):12885-1

Insert

TLN1 (Tln1 Mouse)

Tags

His8-GFP

Expression

Mammalian

Mutation

Lipid binding deficient mutations

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pMpGWB125

Plasmid

#68579

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Triple repeats of Citrine (3xCitrine)

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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Split-RESCUE-N

Plasmid

#170153

Purpose

AAV vector carrying the MCP-RESCUE-DDN (RESCUE is the evolved ADAR2 variant for C-U editing) with a nuclear export signal

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

NES-MCP-RESCUE-DDN

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pHH0103_UBASH3A-1/1

Plasmid

#91478

Purpose

Protein expression and purification of human SH3 domain construct UBASH3A-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

UBASH3A-1/1 (UBASH3A Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pLSU4mg10

Plasmid

#126079

Purpose

Plant transformation vector for stable expression of mitochondria targeted GFP1-10 in plant cells

Depositor

Ralf-Bernd Klösgen

Article

Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

Insert

mitochondria Rieske iron sulphur protein (1-100 aa)

Use

Binary

Tags

GFP1-10

Expression

Plant

Promoter

'long' CaMV35S

Available Since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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CMV-Cx35-Halo-C-in

Plasmid

#71511

Purpose

Mammalian expression plasmid encoding perch Cx35 with an internal HaloTag between amino acids 285 and 286 in the C-terminus.

Depositor

John O'Brien

Article

Wang et al J Cell Sci. 2015 Nov 1;128(21):3888-97.

Insert

Connexin 35

Tags

HaloTag (internal to sequence)

Expression

Mammalian

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pHH0103_NCK1-3/3

Plasmid

#91476

Purpose

Protein expression and purification of human SH3 domain construct NCK1-3/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

NCK1-3/3 (NCK1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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MET FLAG103QDProCFPp303

Plasmid

#15591

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 103Q Delta Pro (HTT Human)

Tags

CFP and FLAG

Expression

Yeast

Mutation

Proline-rich region removed

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pHH0103_SASH1-1/1

Plasmid

#91347

Purpose

Protein expression and purification of human SH3 domain construct SASH1-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SASH1-1/1 (SASH1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pMM1162

Plasmid

#112535

Purpose

pZE2-hrtR-RBS3-chuA - LuxCDABE transcriptional unit deletion of plasmid pMM643, ColE1 origin, KanR

Depositor

Timothy Lu

Article

Mimee et al Science. 2018 May 25;360(6391):915-918.

Inserts

HrtR
ChuA

Expression

Bacterial

Promoter

J23107 and ProD

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pJH3409

Plasmid

#179574

Purpose

Pceh-12::tomm20::miniSOG-SL2::RFP unc-54 3' UTR C.elegans VB-MN -specific expression of miniSOG-SL2 RFP

Depositor

Mei Zhen

Article

Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

Insert

tomm20::miniSOG (tomm-20 Nematode)

Tags

wCherry

Expression

Worm

Promoter

Pceh-12

Available Since

March 1, 2022

Availability

Academic Institutions and Nonprofits only

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pHH0103_SLA2-1/1

Plasmid

#91450

Purpose

Protein expression and purification of human SH3 domain construct SLA2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

SLA2-1/1 (SLA2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_NCK2-2/3

Plasmid

#91362

Purpose

Protein expression and purification of human SH3 domain construct NCK2-2/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

NCK2-2/3 (NCK2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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MS2-adRNA (3'UAG)

Plasmid

#170128

Purpose

Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a UAG at the 3' end of the ADAR2 deaminase domain in plasmids #170124 and 170125

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

MS2-ADAR2(3'UAG)-MS2

Use

Lentiviral

Expression

Mammalian

Promoter

Human U6 and mouse U6

Available Since

May 5, 2022

Availability

Academic Institutions and Nonprofits only

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pRSM14

Plasmid

#101645

Purpose

Encodes the fluorescent protein efasCFP for constitutive expression in E. coli.

Depositor

Thomas Hughes

Article

Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

Insert

efasCFP

Tags

6XHis

Expression

Bacterial

Available Since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pHH0103_NCK1-1/3

Plasmid

#91472

Purpose

Protein expression and purification of human SH3 domain construct NCK1-1/3

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

NCK1-1/3 (NCK1 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR1CB0112

Plasmid

#117548

Purpose

Level 1 Golden Gate Cassette: xCas9 3.7 expression cassette for dicotyledonous plants

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

2x35S+5'UTR OMEGA (pICH51288) + Sp-xCas9 3.7 (no stop codon) (pEPOR0SP0011) + YFP (pICSL50005) + Nos (pICH41421)

Expression

Plant

Promoter

2x35S+5'UTR OMEGA (pICH51288)

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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