Addgene

We narrowed to 21,916 results for: Sis

Showing: 2461 - 2480 of 21916 results

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pCW57.1 blast OFF 3xflag IREB2

Plasmid

#169889

Purpose

Express 3x flag IREB2, suppressible by doxycycline addition

Depositor

Richard Possemato

Article

Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

Insert

IREB2 (IREB2 Human)

Use

Lentiviral; Doxycycline mediated suppression

Tags

3x Flag

Expression

Mammalian

Mutation

Silent mutations in sgIREB2_1 and sgIREB2_2 targe…

Promoter

TRE

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCJ202

Plasmid

#162675

Purpose

pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating W159C and S238C mutations.

Depositor

Gregg Beckham

Article

Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

Insert

PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1)

Use

Tags

His

Expression

Bacterial

Mutation

W159C, S238C; codon optimized for expression in E…

Promoter

T7/lac

Available since

Feb. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pSLIK 3xHA-MKK6-EE neo

Plasmid

#47546

Purpose

Lentiviral expression vector for an inducible 3xHA-tagged constitutively active MKK6 mutant

Depositor

Kevin Janes

Article

Jensen et al Cell Host Microbe. 2013 Jan 16;13(1):67-

Insert

MKK6-EE (MAP2K6 Human)

Use

Lentiviral

Tags

3XHA

Expression

Mammalian

Mutation

S207E, T211E

Promoter

Available since

Sept. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pMRX-IP-muGFP-hATG2A ΔWIR

Plasmid

#215507

Purpose

Expresses GFP tagged human ATG2A with the mutation in WIPI interacting region.

Depositor

Noboru Mizushima

Article

Shimizu et al Hum Mol Genet. 2023 Aug 7;32(16):2623-26

Insert

Autophagy related 2A (ATG2A Human)

Use

Retroviral

Tags

muGFP

Expression

Mammalian

Mutation

P656R (natural variant with no functional relevan…

Promoter

Available since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCW57.1 ISCU D71A

Plasmid

#169880

Purpose

Expresses ISCU iron-sulfur cluster trapping mutant (dominant negative)

Depositor

Richard Possemato

Article

Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

Insert

ISCU (ISCU Human, Synthetic)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Codon Optimized, D71A iron-sulfur cluster trappin…

Promoter

TRE

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pSLIK MEK5-DD-mRFP1 neo

Plasmid

#47548

Purpose

Lentiviral expression vector for an inducible mRFP1-tagged constitutively active MEK5 mutant

Depositor

Kevin Janes

Article

Jensen et al Cell Host Microbe. 2013 Jan 16;13(1):67-

Insert

MEK5-DD-mRFP1 (MAP2K5 Human)

Use

Lentiviral

Tags

mRFP

Expression

Mammalian

Mutation

S311D, T315D

Promoter

Available since

Aug. 28, 2013

Availability

Academic Institutions and Nonprofits only
pET22-His-TEV-DOT1L-MM

Plasmid

#89611

Purpose

Bacterial expression vector for an N-terminally His-tagged (TEV-cleavable) version of human Dot1L hypermorphic MM mutant residues 1-416

Depositor

Leemor Joshua-Tor

Article

Ipsaro et al PLoS One. 2017 Feb 23;12(2):e0172177. do

Insert

Dot1L VVEL293MM Mutant (DOT1L Human)

Use

Tags

His-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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SunTag-FWAgRNA-4-14aa-TET1cd

Plasmid

#106436

Purpose

SunTag CRISPR cas9 system that targets the TET1 catalytic domain to the FWA promoter

Depositor

Steven Jacobsen

Article

Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

Insert

gRNA4_U6_NOS_TET1CD_2xNLS_1xHA__sfGFP_scFv_UBQ10_INSULATOR_UBQ10_dCAS9_1xHA_3xNLS_10xGCN414aa_OCS (TET1 Human, Synthetic, Mustard Weed)

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-CARD14-E138A

Plasmid

#69921

Purpose

Expresses mutant FLAG-CARD14 in mammalian cells

Depositor

Francesca Capon

Article

Berki et al J Invest Dermatol. 2015 Jul 23. doi: 10.

Insert

CARD14 isoform 3 (CARD14 Human)

Use

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

E138A

Promoter

CMV

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3-CARD14-G117S

Plasmid

#69920

Purpose

Expresses mutant FLAG-CARD14 in mammalian cells

Depositor

Francesca Capon

Article

Berki et al J Invest Dermatol. 2015 Jul 23. doi: 10.

Insert

CARD14 isoform 3 (CARD14 Human)

Use

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

G117S

Promoter

CMV

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA3-CARD14-D176H

Plasmid

#69919

Purpose

Expresses mutant FLAG-CARD14 in mammalian cells

Depositor

Francesca Capon

Article

Berki et al J Invest Dermatol. 2015 Jul 23. doi: 10.

Insert

CARD14 isoform 3 (CARD14 Human)

Use

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

D176H

Promoter

CMV

Available since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only
pCJ197

Plasmid

#162670

Purpose

pET-21b(+) based plasmid for expression of MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1), codon optimized for expression in E. coli K12, with C-terminal His tag, incorporating S131G mutation.

Depositor

Gregg Beckham

Article

Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

Insert

MHETase from Ideonella sakaiensis 201-F6 (Genbank GAP38911.1

Use

Tags

His

Expression

Bacterial

Mutation

S131G; codon optimized for expression in E. coli …

Promoter

T7/lac

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV2-TBG-FLAG-mRIPK1-S321A

Plasmid

#115342

Purpose

Liver-specific adeno-associated viral delivery and mammalian expression of Flag-mRIPK1-S321A

Depositor

Junying Yuan

Article

Geng et al Nat Commun. 2017 Aug 25;8(1):359. doi: 1

Insert

RIPK1 (Ripk1 Mouse)

Use

AAV

Tags

Flag

Expression

Mammalian

Mutation

S321A

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV2-TBG-FLAG-mRIPK1-S321E

Plasmid

#115343

Purpose

Liver-specific adeno-associated viral delivery and mammalian expression of Flag-mRIPK1-S321E

Depositor

Junying Yuan

Article

Geng et al Nat Commun. 2017 Aug 25;8(1):359. doi: 1

Insert

RIPK1 (Ripk1 Mouse)

Use

AAV

Tags

Flag

Expression

Mammalian

Mutation

S321E

Promoter

Available since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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SKM2 X057_pECIA2

Plasmid

#115114

Purpose

Bait vector SKM2 X057_pECIA2 should be used with prey vector SKM2 X057_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT5G56040 (AT5G56040 Mustard Weed)

Use

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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BAM1 X060_pECIA2

Plasmid

#115117

Purpose

Bait vector BAM1 X060_pECIA2 should be used with prey vector BAM1 X060_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT5G65700 (BAM1 Mustard Weed)

Use

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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PXC3 X011_pECIA2

Plasmid

#115118

Purpose

Bait vector PXC3 X011_pECIA2 should be used with prey vector PXC3 X011_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT2G41820 (AT2G41820 Mustard Weed)

Use

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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EFR D5_pECIA2

Plasmid

#115119

Purpose

Bait vector EFR D5_pECIA2 should be used with prey vector EFR D5_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT5G20480 (EFR Mustard Weed)

Use

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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FLS2 FLS2_pECIA2

Plasmid

#115120

Purpose

Bait vector FLS2 FLS2_pECIA2 should be used with prey vector FLS2 FLS2_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT5G46330 (FLS2 Mustard Weed)

Use

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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CEPR2 X048_pECIA2

Plasmid

#115099

Purpose

Bait vector CEPR2 X048_pECIA2 should be used with prey vector CEPR2 X048_pECIA14.

Depositor

Youssef Belkhadir

Article

Smakowska-Luzan et al Nature. 2018 Jan 18;553(7688):342-346. d

Insert

AT1G72180 (AT1G72180 Mustard Weed)

Use

Tags

Fc, V5 and hexahistidine tags

Expression

Insect

Mutation

Promoter

Metallothionein (Copper-Inducible)

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge