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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 15,186 results for: egf

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Showing: 2461 - 2480 of 15186 results

  6. Click editor (CE1) - pCMV-T7-PCV2-nCas9-EcKlenow (EMK488)

    Plasmid
    #208942
    Purpose
    The CE1 construct, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Publication
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PCV2-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)
    Promoter
    CMV and T7
    Available since
    Nov. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    208942-plasmid-map-sequence-id-415667

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  7. pDAS12489_PEAR-GFP_2in1_2.0

    Plasmid
    #177186
    Purpose
    PEAR-GFP3 plasmid with a pegRNA that targets its own plasmid
    Depositor
    Ervin Welker
    Publication
    Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    EGFP split with an intron between amino acids 95-96
    pegRNA targeting its own plasmid
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    disrupted 5' splice site
    Promoter
    CMV and U6
    Available since
    Feb. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    177186-plasmid-map-sequence-id-348478

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  8. pCer-C3-Plk1-T210D

    Plasmid
    #68133
    Purpose
    Expression of Cerulean-tagged, constitutively active Plk1 in mammalian cells
    Depositor
    Catherine Lindon
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Polo-like kinase 1 (PLK1 Human)
    Tags
    Cerulean
    Expression
    Mammalian
    Mutation
    T210D constitutively active version
    Promoter
    CMV
    Available since
    Oct. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    68133-plasmid-map-sequence-id-123512

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  14. GeneWeld Vectors for Targeted Integration Using CRISPR/Cas9

    Plasmid kit
    #1000000154
    Purpose
    Targeted genome integration in human cells, zebrafish embryos, and pig fibroblasts.
    Depositor
    Jeffrey Essner
    Application
    Genome Editing
    Vector type
    Mammalian Expression, Zebrafish Expression
    Editing type
    CRISPR
    Available since
    Aug. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
Showing: 2461 - 2480 of 15186 results