We narrowed to 150,436 results for: ins

1. pAB064d

   Plasmid

   #79197

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   Plac62 luxAB; Ppro1 434cI-SH2ABL1
   

   Expression

   Bacterial

   Promoter

   Plac62; Ppro1

   Available Since

   Dec. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTF-lacZ-rfp

   Plasmid

   #153037

   Purpose

   CRISPR-Cas12a genome editing in E. coli. Contains a constitutively CRISPR array targeting the lacZ gene in E. coli genome and donor DNA to insert an constitutively expressed rfp reporter gene.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

   Insert

   CRISPR array
   

   Expression

   Bacterial

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. MSCV-Nkx2-1/Puro

   Plasmid

   #31271

   Depositor

   Tyler Jacks, Monte Winslow

   Article

   Winslow et al Nature. 2011 May 5. 473(7345):101-4.

   Insert

   Nkx2-1* (Nkx2-1 Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Promoter

   MSCV

   Available Since

   Sept. 27, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. Desmoglein2-mCherry

   Plasmid

   #36991

   Depositor

   Kathleen Green

   Article

   Nekrasova et al J Cell Biol. 2011 Dec 26;195(7):1185-203

   Insert

   Dsg2 (DSG2 Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   May 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAB082a

   Plasmid

   #79233

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   Ppro1 rpoZ-Cry1Ac-d123
   

   Expression

   Bacterial

   Promoter

   Ppro1

   Available Since

   Dec. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV_Stargazin-mTurquoise2-iLID

   Plasmid

   #161001

   Purpose

   Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to Stargazin for slow diffusion

   Depositor

   Sean R Collins

   Article

   Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

   Insert

   Stargazin-mTurquoise2-iLID
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. TBP6.7 non-targeting gRNA lambda phage

   Plasmid

   #132547

   Purpose

   expresses gRNA for TBP-based CIRTS

   Depositor

   Bryan Dickinson

   Article

   Rauch et al Cell. 2019 Jun 27;178(1):122-134.e12. do

   Insert

   gRNA TBP-based CIRTS
   

   Expression

   Mammalian

   Promoter

   hU6 promoter

   Available Since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTF-lacZ

   Plasmid

   #153036

   Purpose

   For CRISPR-Cas12a genome editing in E. coli for the insertion of large DNA fragments at the lacZ locus. Contains a constitutively expressed CRISPR array targeting the lacZ gene . No cargo DNA.

   Depositor

   Nigel Scrutton

   Article

   Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.

   Insert

   CRISPR array
   

   Expression

   Bacterial

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-memRFP

   Plasmid

   #224564

   Purpose

   Ubiquitous expression plasmid, contains CAG promoter (CMV immediate early enhancer and chicken beta actin promoter), MCS and membrane RFP reporter.

   Depositor

   Erica Hutchins

   Article

   Kim et al Dev Biol. 2024 Nov 30:S0012-1606(24)0026

   Insert

   mRFP1
   

   Use

   Unspecified

   Tags

   Membrane localization signal

   Mutation

   Codon optimized

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMH-HA/Joseph2-Multitagged Plasmid

    Plasmid

    #226726

    Purpose

    Validates the expression and detection of epitope-tagged proteins of interest in mammalian cells.

    Depositor

    Richard Cripps

    Article

    Robinson et al Biotechniques. 2024;76(7):299-309. doi:

    Insert

    pMH-HA/Joseph2
    

    Tags

    HA, Myc, GFP, Flag, V5, His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 24, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pAB061i

    Plasmid

    #79226

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    Ppro1 rpoZ-HA4; PlacIq lacI
    

    Expression

    Bacterial

    Promoter

    Ppro1; PlacIq

    Available Since

    Oct. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSb_init containing the MBP sybody Sb_MBP#1

    Plasmid

    #132699

    Purpose

    expresses the MBP sybody Sb_MBP#1 in E.coli, to use as positive control in ELISA

    Depositor

    Markus Seeger

    Article

    Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

    Insert

    sybody_MBP#1
    

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. p6618 MSCV-IP N-HAonly WHSC2

    Plasmid

    #35014

    Depositor

    Peter Howley

    Article

    White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

    Insert

    WHSC2 (NELFA Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Promoter

    MSCV LTR

    Available Since

    Feb. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

14. DT0

    Plasmid

    #80418

    Purpose

    Expresses 0 CUG repeats in the context of human DMPK genomic segment containing exons 11-15 with repeats inserted at site in exon 15 that contains the repeats

    Depositor

    Thomas Cooper

    Article

    Ho et al EMBO J. 2004 Aug 4;23(15):3103-12. Epub

    Insert

    human DMPK exons 11-15 with 0 CTG repeats (DMPK Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAB061i3

    Plasmid

    #79230

    Purpose

    Directed Evolution of Protein-Protein Interactions

    Depositor

    David Liu

    Article

    Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

    Insert

    Ppro1 rpoZ; PlacIq lacI
    

    Expression

    Bacterial

    Promoter

    Ppro1; PlacIq

    Available Since

    Nov. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRS41K-mScarlet-I-mNeonGreen (pKBJ010)

    Plasmid

    #173461

    Purpose

    C-SWAT donor for Seamless tagging, contains mScarletI-mNeonGreen tandem fluorescent protein timer

    Depositor

    Anton Khmelinskii

    Article

    Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

    Insert

    mScarlet-I-mNeonGreen
    

    Expression

    Yeast

    Promoter

    no promoter

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIVT-dCas9-mCherry

    Plasmid

    #174443

    Purpose

    in vitro transcription template for dCas9-mCherry

    Depositor

    Michael Lampson

    Article

    Kumon et al Cell. 2021 Sep 16;184(19):4904-4918.e11.

    Insert

    Cas9 Endonuclease Dead
    

    Use

    In vitro transcription template

    Tags

    mCherry

    Promoter

    T7 SP6

    Available Since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT7-EGFP-C1-HsTNRC6A

    Plasmid

    #25035

    Depositor

    Elisa Izaurralde

    Article

    Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

    Insert

    TNRC6A (TNRC6A Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    June 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIVT-dCas9-EGFP

    Plasmid

    #174442

    Purpose

    in vitro transcription template for dCas9-EGFP

    Depositor

    Michael Lampson

    Article

    Kumon et al Cell. 2021 Sep 16;184(19):4904-4918.e11.

    Insert

    Cas9 Endonuclease Dead
    

    Use

    In vitro transcription template

    Tags

    EGFP

    Promoter

    T7 SP6

    Available Since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLV_ADRB2-mTurquoise2-iLID

    Plasmid

    #161002

    Purpose

    Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to ADRB2 for slow diffusion

    Depositor

    Sean R Collins

    Article

    Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

    Insert

    ADRB2-mTurquoise2-iLID
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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