We narrowed to 10,667 results for: plasmids 101
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Plasmid#171585PurposeExpression of 75 nt of the SARS-CoV-2 Leader sequence at 5' of destabilized eGFP reporterDepositorInsertTRS-Leader-SARS-CoV-2-d2eGFP
UseLentiviralTags2PEST AND NLSAvailable SinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4TO-3XFLAG Halo K164A/H165A nsp1
Plasmid#175421PurposeExpresses K164A/H165A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.DepositorInsertCoV2 nsp1
Tags3XFLAG HaloExpressionMammalianPromoterCMVAvailable SinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-NOS-IN133.3xFLAG.mCherry-WPRE
Plasmid#127868PurposepAAV plasmid expressing an NOS-IN133.3xFLAG.mCherry fusion protein under the hSyn promoterDepositorInsertNos1 (Nos1 Mouse)
UseAAVTags3xFLAG and mCherryMutationAmino acids 1-133 onlyPromoterhSynAvailable SinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET30a-SARS-CoV2-ORF3b (S23Q)
Plasmid#165088Purposeprotein expression in E. coliDepositorInsertSevere acute respiratory syndrome coronavirus 2 ORF3b (S23Q)
ExpressionBacterialMutationStop codon to glutamine at 23rd amino acidAvailable SinceFeb. 10, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJS699
Plasmid#168779PurposeEncodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) and can be combined with pJS697 through homologous recombinationDepositorAvailable SinceAug. 31, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1-6xHis-TEV-Nsp8
Plasmid#201022PurposeBacterial expression of codon optimized SARS-CoV-2 nsp8 tagged with an N-terminus His-tag followed by a TEV protease cleavage site.DepositorInsertnon-structural protein 8 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
Tags6xHis-TEVExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable SinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
PAK4 gRNA (BRDN0001147629)
Plasmid#77573Purpose3rd generation lentiviral gRNA plasmid targeting human PAK4DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PAK4 gRNA (BRDN0001148325)
Plasmid#77576Purpose3rd generation lentiviral gRNA plasmid targeting human PAK4DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PAK4 gRNA (BRDN0001146148)
Plasmid#77574Purpose3rd generation lentiviral gRNA plasmid targeting human PAK4DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PAK4 gRNA (BRDN0001145608)
Plasmid#77575Purpose3rd generation lentiviral gRNA plasmid targeting human PAK4DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
MAP3K19 gRNA (BRDN0001146020)
Plasmid#76693Purpose3rd generation lentiviral gRNA plasmid targeting human MAP3K19DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCHC036 (∆pHG1)
Plasmid#226206PurposeConjugation knockout plasmid (pK18msB) to delete the C. necator megaplasmid replication region containing helD, repA, repB, and parAB in order to promote loss of the entire pHG1 megaplasmid.DepositorInsertHomology arms for the pHG1 megaplasmid replication region
UseSynthetic BiologyMutation∆helD ∆repA ∆repB ∆parABAvailable SinceNov. 14, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pVPSBA01-SARS-CoV2-nsp1
Plasmid#192171Purposeexpress SARS2-NSP1DepositorAvailable SinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-AcrIIA1(CTD)-NLS(SV40) (KAC438)
Plasmid#133796PurposeCMV and T7 promoter expression plasmid for human codon optimized AcrIIA1 (truncated C-terminal domain only) with C-terminal NLS (SV40)DepositorInserthuman codon optimized AcrIIA1 (truncated C-terminal domain only)
TagsNLS(SV40)ExpressionMammalianMutationC-terminal domain onlyPromoterCMV and T7Available SinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-AcrIIA1(T114A/F115A)-NLS(SV40) (KAC688)
Plasmid#133798PurposeCMV and T7 promoter expression plasmid for human codon optimized AcrIIA1(T114A/F115A) with C-terminal NLS (SV40)DepositorInserthuman codon optimized AcrIIA1(T114A/F115A)
TagsNLS(SV40)ExpressionMammalianMutationT114A/F115APromoterCMV and T7Available SinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-NOS-IN133.3xFLAG-WPRE
Plasmid#127869PurposepAAV plasmid expressing an NOS-IN133.3xFLAG fusion protein under the hSyn promoterDepositorAvailable SinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
PFKFB2 gRNA (BRDN0001145863)
Plasmid#77685Purpose3rd generation lentiviral gRNA plasmid targeting human PFKFB2DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PFKFB2 gRNA (BRDN0001146926)
Plasmid#77686Purpose3rd generation lentiviral gRNA plasmid targeting human PFKFB2DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PFKFB2 gRNA (BRDN0001147260)
Plasmid#77687Purpose3rd generation lentiviral gRNA plasmid targeting human PFKFB2DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
PFKFB2 gRNA (BRDN0001147256)
Plasmid#77688Purpose3rd generation lentiviral gRNA plasmid targeting human PFKFB2DepositorAvailable SinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only