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Plasmid#72888Purposefusion protein of Gaussia luciferase, Channelrhodopsin-2, and enhanced yellow fluorescent protein for bioluminescent optogeneticsDepositorInsertGaussia luciferase, Channelrhodopsin-2, EYFP
UseTagsExpressionBacterial and MammalianMutationPromoterCAGAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBZcsx27
Plasmid#89900PurposeExpresses csx27 from B. zoohelcum ATCC 43767 in bacteria. Expression driven from the Lac promoterDepositorInsertcsx27
UseTagsExpressionBacterialMutationPromoterLacAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWPXL-Hes1
Plasmid#36983DepositorInsertHes1 (Hes1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pH7WG2B-OsMIR390-B/c
Plasmid#61465PurposePlant expression vector (OsUBI promoter) for direct cloning of artificial microRNAs into rice MIR390 precursor. To use for expressing amiRNAs in monocots.DepositorInsertOsMIR390-B/c
UseTagsExpressionPlantMutationO. sativa MIR390 precursor sequence including a c…PromoterOsUBIAvailable sinceMarch 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTol1-UAS:ChR2(H134R)-tdTomato
Plasmid#124237PurposeZebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin ChR2(H134R) fused to the red fluorescent protein tdTomatoDepositorInsertChR2(H134R)-tdTomato
UseZebrafish expressionTagstdTomatoExpressionMutationPromoter14xUAS-E1bAvailable sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET24a-VHH-myc
Plasmid#109422PurposeBacterial expression of a functionalized anti-GFP (VHH) nanobody fused to a myc epitope. VHH-myc also contains a T7, HA, BAP and His6 epitopeDepositorInsertanti-GFP nanobody fused to a T7, myc, HA, BAP and His6 epitope
UseTagsBAP, HA, His6, T7, and mycExpressionBacterialMutationPromoterT7Available sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
rAAV-CAG::FLEX-rev:ChR2HA:2a:PSAML141F,Y115F:GlyR
Plasmid#32483PurposeCre-dependent expression of ChR2 and PSAM-GlyR (L141F,Y115F) neuronal inhibitor, with IRES-EGFP markerDepositorInsertChR2HA-2a-PSAML141F,Y115F:GlyR
UseAAV and Cre/Lox; Adeno-associated virus, flex swi…TagsChR2 2AExpressionMutationPromoterCAGAvailable sinceApril 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBSFI-myr-akt-S473A
Plasmid#49190PurposecDNA of Akt1 with myristyolation signal and point mutation from S to A at position 473 in cloning vectorDepositorInsertAkt1 (Akt1 Mouse)
UseAdaptor plasmidTagsHA tag and myristolation signalExpressionMutationS473APromoterT7Available sinceDec. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBabe-TetCMV-puro-mVenus-PA-Rac1
Plasmid#22008PurposeMammalian expression of photoactivatable Rac1DepositorInsertPA-Rac1 (RAC1 Human, Avena sativa (oat))
UseRetroviralTagsmVenusExpressionMammalianMutationLOV(Leu404-Leu546), Rac1 starts at I4 and contain…PromoterAvailable sinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn1 ChR2 ET-TC 2A tDimer
Plasmid#101361PurposeHigh efficiency channelrhodopsin, neuron specific promoter, red fluorescent proteinDepositorInsertsChannelrhodopsin-2
synaptophysin-red fluorescent protein
UseAAVTagsExpressionMammalianMutationE123T , T159CPromoterhuman SynapsinAvailable sinceFeb. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pY005 (pcDNA3.1-hLb3Cpf1)
Plasmid#69977PurposeExpresses humanized Lb3Cpf1DepositorInserthLb3Cpf1
UseCRISPRTagsNLS & 3xHAExpressionMammalianMutationPromoterCMVAvailable sinceOct. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MSP1D1deltaH4/2H5
Plasmid#71715PurposeMembrane scaffold protein (MSP), helix 4/2 + helix5 deletionDepositorInsertApolipoprotein A1 (APOA1 Human)
UseTags6xHis and TEV siteExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
JN202: pMVP/SB/Blast-DEST
Plasmid#121864PurposepMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Blast selection cassetteDepositorTypeEmpty backboneUseSleeping beauty transposon, pmvp gateway destinat…TagsExpressionMammalianMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD003 sgKEAP1-2
Plasmid#74986PurposesgKEAP1-2, puromycin selectionDepositorInsertKEAP1
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBSFI-myr-akt-T308A
Plasmid#49189PurposecDNA of Akt1 with myristyolation signal and point mutation from T to A at position 308 in cloning vectorDepositorInsertAkt1 (Akt1 Mouse)
UseAdaptor plasmidTagsHA tag and myristolation signalExpressionMutationT308APromoterT7Available sinceDec. 13, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MSP1D1deltaH4/2
Plasmid#71712PurposeMembrane scaffold protein (MSP), half of helix 4 truncatedDepositorInsertApolipoprotein A1 (APOA1 Human)
UseTags6xHis and TEV siteExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSKA397
Plasmid#80380Purposemini-Tn7 delivery plasmid with metE under the cpcG2Δ59 promoter.DepositorInsertscat
metE
UseSynthetic Biology; Mini-tn7 delivery vectorTagsExpressionBacterialMutationPromotercat promoter and cpcG2Δ59Available sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1α-FRT-FLEX-GtACR2-KA2N-EGFP
Plasmid#114378Purposeexpresses Flpo recombinase-dependent GtACR2-KA2N, which targets GtACR2 to the somatodendritic compartmentDepositorInsertGtACR2-KA2N-EGFP (NEWENTRY )
UseAAVTagsEGFP and KA2NExpressionMammalianMutationPromoterEf1aAvailable sinceAug. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSicoR-Ef1a-mCh-Puro-GFPi
Plasmid#31848DepositorInsertGFP RNAi
UseCre/Lox, Lentiviral, and RNAiTagsExpressionMammalianMutationPromoterU6 for shRNA; Ef1alpha for mCherry-puromycinAvailable sinceJan. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMDC32B-OsMIR390-B/c
Plasmid#61467PurposePlant expression vector (2x35S) for direct cloning of artificial microRNAs into rice MIR390 precursor. To use for expressing amiRNAs in monocots.DepositorInsertOsMIR390-B/c
UseTagsExpressionPlantMutationO. sativa MIR390 precursor sequence including a c…Promoter2x35SAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only