We narrowed to 23,252 results for: Sele

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pDRM98 mKate2 Blast

Plasmid

#174720

Purpose

Expresses the NLS-tagged mKate2 gene, P2A and the blasticidin resistance gene.

Depositor

Wayne Tilley

Article

Mustafa et al Oncogene. 2023 Nov 24. doi: 10.1038/s413

Inserts

mKate2
BSD

Use

Lentiviral

Tags

P2A linker and SV40 NLS with GGS linker

Expression

Mammalian

Promoter

MND and MND (off mKate-P2A)

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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MEF2D-2A-neo

Plasmid

#190622

Purpose

Lentiviral expression plasmid of human MEF2D (long isoform), neomycin selection

Depositor

Junwei Shi

Article

Pingul et al iScience. 2022 Sep 15;25(10):105139. doi

Insert

MEF2D (MEF2D Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pST_116_LVL2 cam

Plasmid

#179332

Purpose

NT-CRISPR plasmid for a single gRNA.

Depositor

Anke Becker

Article

Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

Insert

Ptac tfoX, Ptet cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout

Use

CRISPR

Available Since

March 21, 2022

Availability

Academic Institutions and Nonprofits only

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pIGc-Xph20

Plasmid

#133024

Purpose

Expresses Xph20 in bacteria

Depositor

Matthieu Sainlos

Article

Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

Insert

Xph20

Tags

His10

Expression

Bacterial

Mutation

S63K

Promoter

T7

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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MLNR-DuET

Plasmid

#213339

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

MLNR (MLNR Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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HA-GABARAP

Plasmid

#137760

Purpose

Expression of GABARAP

Depositor

Carol Mercer

Article

Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

Insert

GABARAP (GABARAP Human)

Tags

HA and myc

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-hPOMC1-26-sbGLuc-P2A-dTomato

Plasmid

#176704

Purpose

neural activity dependent release of sbGLuc from presynaptic vesicles

Depositor

Ute Hochgeschwender

Article

Prakash et al Commun Biol. 2022 Jan 11;5(1):33. doi: 1

Inserts

Gaussia luciferase, slow burn
dTomato

Use

AAV

Tags

P2A and hPOMC1-26

Promoter

hSyn

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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TOM20MTS-mCherry-EGFP-Tet-On

Plasmid

#109016

Purpose

Tet-On construct to induce expression of tandem eGFP-mCherry tagged TOM20MTS (mitochondria

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

TOM20MTS (MTS: mitochondria targeting signal) (TOMM20 Human)

Use

Lentiviral

Tags

mCherry-eGFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCDH-SIRT2-Flag

Plasmid

#102624

Purpose

Expresses full length SIRT2 isoform 1 in mammalian cells

Depositor

Hening Lin

Article

Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

Insert

SIRT2 (SIRT2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2018

Availability

Academic Institutions and Nonprofits only

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SSTR4-DuET

Plasmid

#213379

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

SSTR4 (SSTR4 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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eSpCas9(1.1)_No_FLAG_NPM1_G6

Plasmid

#178091

Purpose

Expresses the NPM1 G6 sgRNA in combination with FLAGless eSpCas9(1.1). This vector can be used in combination with NPM1_mNeonGreen_Donor to tag NPM1 with mNeonGreen. pX330-like plasmid.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

NPM1 G6 sgRNA + FLAGless enhanced specificity Cas9 (1.1)

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoter

Available Since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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PMP34-mCherry-EGFP-Tet-On

Plasmid

#109017

Purpose

Tet-On construct to induce expression of tandem mCherry-eGFP tagged PMP34 (peroxisome marker)

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

PMP34 (SLC25A17 Human)

Use

Lentiviral

Tags

mCherry-eGFP

Expression

Mammalian

Promoter

CMV promoter

Available Since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-A3

Plasmid

#135505

Purpose

Mammalian expression of myc-tagged HLA-A*03:01

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-A*03:01 (HLA-A Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-HA-Nrxn1α-LNS2

Plasmid

#128106

Purpose

Express HA tagged Nrxn1α-LNS2

Depositor

Huda Zoghbi

Article

Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

Insert

Nrxn1a-LNS2 (Nrxn1 Rat)

Tags

HA

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-HA-Nrxn1α-LNS4

Plasmid

#128108

Purpose

Express HA tagged Nrxn1α-LNS4

Depositor

Huda Zoghbi

Article

Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

Insert

Nrxn1a-LNS4 (Nrxn1 Rat)

Tags

HA

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-HA-Nrxn1α-LNS5

Plasmid

#128109

Purpose

Express HA tagged Nrxn1α-LNS5

Depositor

Huda Zoghbi

Article

Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

Insert

Nrxn1a-LNS5 (Nrxn1 Rat)

Tags

HA

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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Lifeact-7-iRFP670

Plasmid

#103032

Purpose

Expression of iRFP670-tagged Lifeact with G418 selection in mammalian cells

Depositor

Ghassan Mouneimne

Article

Padilla-Rodriguez et al Nat Commun. 2018 Jul 30;9(1):2980. doi:

Insert

Lifeact

Tags

iRFP670

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2018

Availability

Academic Institutions and Nonprofits only

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PET-eSpCas9-NLS-6xHis

Plasmid

#207379

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

eSpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

K848A, K1003A, R1060A

Promoter

T7

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pST_119_LVL2 cam

Plasmid

#179333

Purpose

NT-CRISPR plasmid for integration of multiple gRNAs.

Depositor

Anke Becker

Article

Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

Insert

Ptac tfoX, Ptet cas9, PJ23106 acrIIA4, sfGFP dropout to be replaced with gRNAs

Use

CRISPR

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pCLXEBR-pTF-Ngn3

Plasmid

#114307

Purpose

TET-inducible expression of the Neugenin 3 transcription factor

Depositor

Patrick Salmon

Article

Giry-Laterriere et al Hum Gene Ther. 2011 Oct;22(10):1255-67.

Insert

Ngn3 (NEUROG3 Human)

Use

Lentiviral

Available Since

Sept. 11, 2018

Availability

Academic Institutions and Nonprofits only

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