We narrowed to 11,276 results for: Vars

1. pET28a_PA4114_Cterm_His

   Plasmid

   #220484

   Purpose

   heterologous expression of PA4114 for protein purification

   Depositor

   Emily Balskus

   Article

   Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

   Insert

   PA4114
   

   Use

   Tags

   6xHs

   Expression

   Bacterial

   Mutation

   none

   Promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTW211b5

   Plasmid

   #136927

   Purpose

   Directed evolution of SpCas9

   Depositor

   David Liu

   Article

   Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

   Insert

   Cas9(N)-NpuN
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   see manuscript

   Promoter

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKSB-609 U6-sgRNA1 ATCC GGAA

   Plasmid

   #173671

   Purpose

   To clone and express a gRNA in Anopheles

   Depositor

   Eric Marois

   Article

   Klug et al PLoS Pathog. 2022 Oct 12;18(10):e1010881

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJYDNgp

   Plasmid

   #162453

   Purpose

   Positive control plasmid for pJYDNg carrying the eUnaG2 bilirubin dependent fluorescent protein fused to the C-terminus of Aga2p protein. This plasmid contains a long 2G linker.

   Depositor

   Gideon Schreiber

   Article

   Zahradník et al bioRxiv Preprint 2020

   Insert

   Aga2p-2Glinker-eUnaG2 (AGA2 )
   

   Use

   Shuttle vector bacteria/yeast

   Tags

   HA nad myc and eUnaG2 fluorescent protein

   Expression

   Mutation

   Promoter

   GAL1

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. GALT-APEX2

   Plasmid

   #215554

   Purpose

   Stable, inducible expression in Golgi

   Depositor

   Nevan Krogan

   Article

   Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

   Insert

   APEX2-NES-GalT-EGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pZH516

   Plasmid

   #102669

   Purpose

   Constitutive expression of GFPmut2; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

   Depositor

   Zach Hensel

   Article

   Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

   Insert

   GFPmut2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. p1.1

   Plasmid

   #162737

   Purpose

   Protein expression in CHO cells

   Depositor

   Ivan Vorobiev

   Article

   Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

   Available since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKSB-610 U6-sgRNA2 GGAA AGAG

   Plasmid

   #173672

   Purpose

   To clone and express a gRNA in Anopheles

   Depositor

   Eric Marois

   Article

   Klug et al PLoS Pathog. 2022 Oct 12;18(10):e1010881

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pZH514

   Plasmid

   #102667

   Purpose

   Constitutive expression of GFPmut2; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

   Depositor

   Zach Hensel

   Article

   Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

   Insert

   GFPmut2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. mTFP1-Lifeact-7

    Plasmid

    #54749

    Purpose

    Localization: Actin, Excitation: 462, Emission: 492

    Depositor

    Michael Davidson, Robert Campbell

    Article

    Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

    Type

    Empty backbone

    Use

    Tags

    mTFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pRDA_450

    Plasmid

    #179094

    Purpose

    nuclease Cas expression

    Depositor

    John Doench, David Root

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    SpRY
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX165-Cas9-HF1

    Plasmid

    #140565

    Purpose

    Expresses Cas9-HF1 in mammalian cells

    Depositor

    Feng Zhang

    Article

    Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mutation

    N497A R661A Q695A Q926A

    Promoter

    Available since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-HA-MAVS-M142A(KaganF61)

    Plasmid

    #51999

    Purpose

    Expresses the human MAVS CDS containing the point mutation M142A and an N-terminal HA tag "strong translational context"

    Depositor

    Jonathan Kagan

    Article

    Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

    Insert

    MAVS
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Met 142 to Ala

    Promoter

    Available since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pX165-Sniper-Cas9

    Plasmid

    #140560

    Purpose

    Expresses Sniper Cas9 in mammalian cells

    Depositor

    Feng Zhang

    Article

    Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mutation

    F539S M763I K890N

    Promoter

    Available since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. VZV ORF47

    Plasmid

    #26698

    Depositor

    Robert Kalejta

    Article

    Kuny et al PLoS Pathog. 2010 . 6(9):.

    Insert

    ORF47 (ORF47 Varicella Zoster Virus (HHV-3))
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    A 3B3 epitope tag (DQRQYGDVFKGD) from the VZV gB …

    Promoter

    Available since

    Nov. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pSADdeltaG-ChR2-mCherry

    Plasmid

    #32646

    Purpose

    Rabies vector pSADΔG expressing ChR2-mCherry for optogenetic excitation

    Depositor

    Edward Callaway

    Article

    Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

    Insert

    ChR2
    

    Use

    Rabies virus

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 26, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJY3919

    Plasmid

    #113332

    Purpose

    Hermes transposable plasmid; TIR-flanked LEU2, recoded transposase driven by TEF1 promoter

    Depositor

    Suzanne Sandmeyer

    Article

    Patterson et al Metab Eng. 2018 May 22;48:184-196. doi:

    Inserts

    beta-isopropyl malate dehydrogenase
    codon-optimized Hermes transposase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBAD/HisD-TagRFP675

    Plasmid

    #44274

    Depositor

    Vladislav Verkhusha

    Article

    Piatkevich et al Sci Rep. 2013;3:1847. doi: 10.1038/srep0

    Insert

    TagRFP675
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    M41Q/F80W/S143N/L147M/S158N/D159Y/N173S

    Promoter

    pBAD

    Available since

    May 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET15b I-SceI (Ctermhis -MH)

    Plasmid

    #195613

    Purpose

    Expresses the wild-type I-SceI homing endonuclease from the T7 promoter

    Depositor

    Frederick Gimble

    Article

    Niu et al J Mol Biol. 2008 Sep 26;382(1):188-202.

    Insert

    I-SceI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    None

    Promoter

    T7

    Available since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. p1.2-Neo

    Plasmid

    #162739

    Purpose

    Protein expression in CHO cells

    Depositor

    Ivan Vorobiev

    Article

    Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CHO Elongation factor 1A

    Available since

    July 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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