We narrowed to 11,626 results for: Vars

1. AAV-flex-oChIEF-citrine

   Plasmid

   #50973

   Purpose

   flexed oChIEF-citrine construct in an AAV transfer vector containing AAV2 ITR

   Depositor

   Roger Tsien

   Article

   Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

   Insert

   ChIEF-citrine
   

   Use

   AAV

   Tags

   citrine

   Mutation

   inverted and inserted between two lox sites

   Promoter

   human synapsin promoter

   Available Since

   Feb. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pU6a:sgRNA(tyr)

   Plasmid

   #64250

   Purpose

   expresses sgRNA(tyr) under U6a promoter

   Depositor

   Wenbiao Chen

   Article

   Yin et al Genetics. 2015 Apr 8. pii: genetics.115.

   Insert

   U6a:sgRNA (tyr)
   

   Use

   CRISPR

   Available Since

   May 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMT3928

   Plasmid

   #113331

   Purpose

   control for Hermes transposition; LEU2 without flanking Hermes TIRs, recoded transposase driven by TEF1 promoter

   Depositor

   Suzanne Sandmeyer

   Article

   Patterson et al Metab Eng. 2018 May 22;48:184-196. doi:

   Inserts

   beta-isopropyl malate dehydrogenase
   codon-optimized Hermes transposase
   

   Use

   Unspecified

   Available Since

   Oct. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hSyn-GluA1-scFv-mNeon-KDEL

   Plasmid

   #230056

   Purpose

   AAV plasmid that expresses a single chain variable fragment (scFv) for anti-GluA1 tagged with mNeon. Encodes an ER retrieval motif (KDEL) at C-terminus of mNeon.

   Depositor

   Matthew Kennedy

   Insert

   Anti-GluA1 single chain variable fragment (scFv)
   

   Use

   AAV

   Tags

   ER retrieval motif (KDEL) and mNeon

   Expression

   Mammalian

   Promoter

   human synapsin

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. DDX3X in pENTR

   Plasmid

   #216572

   Purpose

   gateway cloning donor vector containing DDX3X

   Depositor

   Christopher Loewen, Douglas Allan

   Insert

   DDX3X (DDX3X Human)
   

   Use

   Gateway entry plasmid

   Available Since

   March 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. GeNL(Ca2+)_520/pcDNA3

   Plasmid

   #85204

   Purpose

   luminescent reporter for calcium signaling

   Depositor

   Takeharu Nagai

   Article

   Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

   Insert

   GeNL(Ca2+)_520
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. mWasabi-N1

   Plasmid

   #54765

   Purpose

   Localization: N1 Cloning Vector, Excitation: 493, Emission: 509

   Depositor

   Robert Campbell, Michael Davidson

   Article

   Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

   Type

   Empty backbone

   Tags

   mWasabi

   Expression

   Mammalian

   Available Since

   June 20, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pAGD289

   Plasmid

   #228113

   Purpose

   pET-21(+) deriviative with retron Kva1 containing a donor to edit E. coli rpoB gene cloned downstream of a T7 promoter

   Depositor

   Seth Shipman

   Article

   Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

   Insert

   Kva1 retron, ncRNA with 90 bp rpoB donor
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Endo-APEX2

   Plasmid

   #215553

   Purpose

   Stable, inducible expression in endosome

   Depositor

   Nevan Krogan

   Article

   Zhong et al bioRxiv. 2023 Apr 12:2023.04.11.536358.

   Insert

   EGFP-2xFYVE-APEX2-NES
   

   Use

   Lentiviral

   Available Since

   Aug. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-CamKIIa-moxBFP-WPRE

    Plasmid

    #194976

    Purpose

    AAV vector to drive expression of moxBFP under the control of CamKIIalpha promoter

    Depositor

    Günter Schwarz

    Article

    Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

    Insert

    moxBFP
    

    Use

    AAV

    Available Since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSK-Sasg

    Plasmid

    #214350

    Purpose

    Expresses cloning backbone for SlugCas9 sgRNA

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SlugCas9 tracrRNA
    

    Expression

    Mammalian

    Available Since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTW170b2

    Plasmid

    #136929

    Purpose

    Directed evolution of SpCas9

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    gVI
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    none

    Available Since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. MS2_SC2_S_Delta

    Plasmid

    #175950

    Purpose

    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Delta SARS-CoV-2 S gene (Amino Acid 319-869)

    Depositor

    Paul Freemont

    Inserts

    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    

    Expression

    Bacterial

    Mutation

    Delta (Amino Acid 319-869)

    Promoter

    T7

    Available Since

    Oct. 18, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. PTPN11 in pENTR

    Plasmid

    #216604

    Purpose

    gateway cloning donor vector containing PTPN11

    Depositor

    Christopher Loewen, Douglas Allan

    Insert

    PTPN11 (PTPN11 Human)
    

    Use

    Gateway entry plasmid

    Available Since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-hSyn-GluA1-scFv-Halo

    Plasmid

    #230055

    Purpose

    AAV plasmid that expresses a single chain variable fragment (scFv) for anti-GluA1 fused to halotag. Can be directly transfected into neurons or used to prepare AAV expressing GluA1-scFv-Halo.

    Depositor

    Matthew Kennedy

    Insert

    Anti-GluA1 single chain variable fragment (scFv)
    

    Use

    AAV

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    human synapsin

    Available Since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pECFP-DEVG-Venus

    Plasmid

    #24538

    Depositor

    Peter Sorger

    Article

    Albeck et al PLoS Biol. 2008 Dec 2. 6(12):2831-52.

    Insert

    ECFP-DEVG-Venus
    

    Tags

    ECFP and Venus

    Expression

    Mammalian

    Mutation

    DEVDR mutated to DEVG

    Promoter

    CMV

    Available Since

    June 11, 2010

    Availability

    Academic Institutions and Nonprofits only

17. G417A_pLenti-Tet-BattP(GA)-BFP-2A-iCasp9-2A-Blast_rtTA3

    Plasmid

    #200631

    Purpose

    Negative selection blasticidin resistant Tet-inducible Matreyek Bxb1(GA) landing pad

    Depositor

    Kenneth Matreyek

    Article

    Roelle et al ACS Synth Biol. 2023 Nov 3. doi: 10.1021

    Insert

    iCasp9
    

    Use

    Lentiviral

    Promoter

    pTRE3G

    Available Since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX330A_dCas9-1x2

    Plasmid

    #63596

    Purpose

    Expresses dCas9 and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

    Insert

    humanized S. pyogenes dCas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D1361Y

    Promoter

    CBh

    Available Since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. GeNL(Ca2+)_60/pcDNA3

    Plasmid

    #85207

    Purpose

    luminescent reporter for calcium signaling

    Depositor

    Takeharu Nagai

    Article

    Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

    Insert

    GeNL(Ca2+)_60
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pST-Ki

    Plasmid

    #44563

    Purpose

    for integrative constitutive expression of mycobacterial proteins

    Depositor

    Vinay Nandicoori

    Article

    Parikh et al Appl Environ Microbiol. 2013 Mar;79(5):1

    Type

    Empty backbone

    Use

    E.coli-mycobacteria shuttle vector

    Tags

    Flag tag and His tag

    Expression

    Bacterial

    Available Since

    Oct. 30, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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