We narrowed to 8,345 results for: construct

1. pFGC5941-PacI

   Plasmid

   #44182

   Depositor

   Guiliang Tang

   Article

   Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. PlayBack-CMV-Kpn1

   Plasmid

   #203302

   Purpose

   For expression of eukaryotic genes of interest with CMV promoter with Kpn1 as a PlayBack Restriction Enzyme

   Depositor

   Aleksandar Necakov

   Article

   Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. p{Mhc-F4-678-eGFP::FRT,attB}

   Plasmid

   #182185

   Purpose

   fluorescent reporter gene with yeast recombination site within intron

   Depositor

   David Stern

   Article

   Stern et al G3 (Bethesda). 2022 Feb 10. pii: 6526388

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. PlayBack-Ef1a-EcoR1

   Plasmid

   #203311

   Purpose

   For expression of eukaryotic genes of interest with Ef1a promoter with EcoRI as a PlayBack Restriction Enzyme

   Depositor

   Aleksandar Necakov

   Article

   Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pYZ301

   Plasmid

   #102690

   Purpose

   S. pombe gRNA entry vector with Cas9 - Sp lys9 marker

   Depositor

   Jef Boeke

   Article

   Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pYZ294

   Plasmid

   #102688

   Purpose

   S. pombe gRNA entry vector with Cas9 - KanMX marker

   Depositor

   Jef Boeke

   Article

   Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pYZ300

   Plasmid

   #102689

   Purpose

   S. pombe gRNA entry vector with Cas9 - Sp his3 marker

   Depositor

   Jef Boeke

   Article

   Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pYZ293

   Plasmid

   #102687

   Purpose

   S. pombe gRNA entry vector with Cas9 - Sp leu1 marker

   Depositor

   Jef Boeke

   Article

   Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   April 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGBW-m4134100

   Plasmid

   #152050

   Purpose

   Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;6xHis-003

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   CMV

   Available since

   May 20, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pBSDT-AII

    Plasmid

    #27179

    Depositor

    Yoh Wada

    Article

    Aoyama et al Nucleic Acids Res. 2005 . 33(5):e52.

    Insert

    MC1 promoter diphtheria toxin-A fragment
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAMP-CY1_NF1 T2 (+minintr)

    Plasmid

    #119205

    Purpose

    transfer vector

    Depositor

    Helen Morrison

    Article

    Cui et al Hum Mutat. 2018 Nov 8. doi: 10.1002/humu

    Insert

    NF1 type 2 mini-gene (NF1 Human)
    

    Use

    Transfer vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PL-452 C-EGFP

    Plasmid

    #19178

    Depositor

    Patrik Verstreken

    Article

    Venken et al Nucleic Acids Res. 2008 Aug 1. ():.

    Insert

    EGFP
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pGBW-m4046203

    Plasmid

    #145488

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 3C-like proteinase

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 3C-like proteinase (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;3xFLAG

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pCGN R-Ras wt

    Plasmid

    #14727

    Depositor

    Adrienne Cox

    Article

    Fiordalisi et al Methods Enzymol. 2001 . 332():3-36.

    Insert

    R-ras (RRAS Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 29, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pGBW-m4046233

    Plasmid

    #149032

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 nsp3

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp3 (ORF1ab Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. EF.hICN1

    Plasmid

    #113715

    Purpose

    Constitutive expression of hICN1, e.g. for hedgehog-induction. Based on addgene #17623, with CMV.GFP removed.

    Depositor

    Manfred Ogris

    Article

    Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

    Insert

    None
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGBW-m4046664

    Plasmid

    #149224

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 E (envelope)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 E (envelope) (E Budding Yeast, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pLenti-EF1a-Backbone(NN)

    Plasmid

    #27961

    Depositor

    Feng Zhang

    Article

    Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

    Insert

    TAL-Backbone(NN)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGBW-m4133227

    Plasmid

    #152132

    Purpose

    Mammalian expression plasmid for SARS-CoV-2 surface glycoprotein (Spike protein)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 S (surface glycoprotein) (S Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;3xFlag

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available since

    May 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pSG84-pLas-TetRssrA

    Plasmid

    #102455

    Purpose

    Expresses TetR protein with ssrA degradation tag. The expression is controlled by a pLas promoter.

    Depositor

    Richard Murray

    Article

    Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

    Insert

    TetR (tetR )
    

    Use

    Tags

    ssrA

    Expression

    Bacterial

    Mutation

    Promoter

    pLas

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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