We narrowed to 20,413 results for: INO

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pBactin-EGFP-NEDD1-gTBD

Plasmid

#196857

Purpose

Expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to enhanced (E) GFP. Used to displace endogenous gamma-TuRC from the centrosome.

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

EGFP-N-gTBD (NEDD1 Human)

Expression

Mammalian

Promoter

Chicken bactin (plus Chicken bactin intron)

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pMMK ENTR 2 CMV mKO2-hCdt1

Plasmid

#206282

Purpose

ENTR Vector 2 for MultiSite Gateway assembly. Encodes mKO2-hCdt1 under the control of CMV

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Insert

mKO2-hCdt1 (CDT1 Human)

Use

Multimate/gateway entr 2

Expression

Mammalian

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pBactin-mOrange2-NEDD1-gTBD

Plasmid

#196858

Purpose

Expression of the gamma-tubulin-binding domain (gTBD) of NEDD1 fused to mOrange. Used to displace endogenous gamma-TuRC from the centrosome.

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

mOrange2-N-gTBD (NEDD1 Human)

Expression

Mammalian

Promoter

Chicken bactin (plus Chicken bactin intron)

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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phBG-P53DD

Plasmid

#149707

Purpose

In vitro transcription of dominant negative form of P53 mRNA for RNA transfection into mammalian cells

Depositor

Erika Sasaki

Article

Watanabe et al Genes Cells. 2019 Jul;24(7):473-484. doi

Insert

Trp53 (Trp53 Mouse)

Use

Rna transcription

Mutation

deleted amino acid 14-301

Promoter

T7

Available Since

June 24, 2020

Availability

Academic Institutions and Nonprofits only

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pZE21/UBP1/ClpS

Plasmid

#98566

Purpose

Expresses truncated codon-opt S. cerevisiae UBP1 and E. coli ClpS in an artificial operon

Depositor

George Church

Article

Kunjapur et al Proc Natl Acad Sci U S A. 2018 Jan 16;11

Inserts

Truncated ubiquitin cleavase, codon optimized
ATP-dependent Clp protease adapter protein

Expression

Bacterial

Mutation

In a synthetic operon downstream of UBP1 and Remo…

Promoter

Same as UBP1 (operon) and Tet promoter (aTc induc…

Available Since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pS6-TGFB2-STREP

Plasmid

#128503

Purpose

Expresses human pro-TGFβ2 (from L21 to S414) in mammalian cells. With N-t STREP(2x) tag.

Depositor

F Xavier Gomis-Ruth

Article

Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

Insert

TGFB2 (TGFB2 Human)

Tags

STREP (2X) tag

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry.3xFLAG.NOS1AP396-500-WPRE

Plasmid

#174134

Purpose

pAAV plasmid expressing an mCherry.3xFLAG.NOS1AP396-500 fusion protein under the hSyn promoter

Depositor

Florian Freudenberg

Article

Freudenberg et al EBioMedicine. 2021 Aug 26;71:103565. doi

Insert

Nos1ap (Nos1ap Mouse)

Use

AAV

Tags

3xFLAG and mCherry

Mutation

Only contains the sequences coding for amino acid…

Promoter

hSyn

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pGBT-RP8.1

Plasmid

#189846

Purpose

Intron-based gene-break transposon (GBT) for an effective and revertible loss-of-function tool for zebrafish. Lens tagBFP frameshift 1.

Depositor

Stephen Ekker

Article

Ichino et al Elife. 2020 Aug 11;9. pii: 54572. doi: 1

Type

Empty backbone

Use

Cre/Lox

Tags

mRFP and tagBFP

Promoter

beta Actin

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pIE-C-hα2M-H6

Plasmid

#128494

Purpose

Expresses human C-α2M (from R787 to N1473) in insect cells. With C-t H6x tag.

Depositor

F Xavier Gomis-Ruth

Article

Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

Insert

A2M (R787-N1473) (A2M Human)

Tags

His tag (6x)

Expression

Insect

Mutation

contains R787-N1473

Promoter

IE1

Available Since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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Mouse DsRed-Monomer CASP9 C325A Mutant

Plasmid

#198401

Purpose

Expresses Mouse DsRed-Monomer CASP9 C325A Mutant Fusion Protein in Mammalian Cells

Depositor

Hannah Rabinowich

Article

Han et al Autophagy. 2021 Jun;17(6):1393-1409. doi

Insert

Mouse Caspase-9 C325A Mutant (Casp9 Mouse)

Tags

DsRed-monomer

Expression

Mammalian

Mutation

Changed Cysteine 325 to Alanine to eliminate prot…

Promoter

CMV

Available Since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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mApple-M1 SpastinHFDD

Plasmid

#134462

Purpose

M1 SpastinHFDD expression

Depositor

Jennifer Lippincott-Schwartz

Article

Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

Insert

M1 Spastin (SPAST Human)

Expression

Mammalian

Mutation

Met to Ala at amino acid 87; His to Asp at amino …

Promoter

CMV

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pPICZ:mTREK-1_cryst

Plasmid

#133269

Purpose

P. pastoris expression vector. It will generate the mouse TREK-1 channel (21-322) fused to a C-terminal GFP

Depositor

Dan Minor

Article

Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d

Insert

Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 Mouse)

Tags

SNS- 3C_cleavage_site-TAAA-GFP

Expression

Yeast

Mutation

aa 21-322, K84R, Q85E, T86K, I88L, A89R, Q90A, A9…

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-Sema4D-CD4-FLAG

Plasmid

#51601

Purpose

expresses non-cleavable Sema4D/CD4 chimera with extracellular Sema4D, intracellular CD4

Depositor

Suzanne Paradis

Article

Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

Insert

Sema4D/CD4-FLAG (Sema4d Mouse, Human)

Tags

FLAG and myc

Expression

Mammalian

Mutation

Sema4D amino acids 660-861 were replaced with CD4…

Promoter

CMV

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only
GFP-ataxin-3 C-t

Plasmid

#89981

Purpose

Expresses the GFP-tagged C-terminus of ataxin-3

Depositor

Nico Dantuma

Article

Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

Insert

ataxin-3 (ATXN3 Human)

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 1-182

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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pYPQ223-STU

Plasmid

#138109

Purpose

Gateway entry plasmid (attL1 & attR5) expressing rice codon optimized, catalytically deactivated AsCas12a-SRDX repressor without promoter and terminator

Depositor

Yiping Qi

Article

Zhang et al Nat Commun. 2021 Mar 29;12(1):1944. doi:

Insert

dAsCas12a-SRDX

Use

CRISPR; Gateway compatible cas12a entry clone

Tags

NLS

Expression

Plant

Available Since

March 31, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/NDUFS2 _34-78/myc-His

Plasmid

#127496

Purpose

Expresses myc-tagged _34-78 NDUFS2 in mammalian cells

Depositor

Shashi Jain, Dieter Wolf

Article

Jain et al Cell Chem Biol. 2021 Nov 23. pii: S2451-

Insert

NDUFS2 _34-78 (NDUFS2 Human)

Tags

myc-His

Expression

Mammalian

Mutation

NDUFS2 _34-78

Promoter

CMV

Available Since

July 16, 2019

Availability

Academic Institutions and Nonprofits only

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pCELF PAI-RBP v3

Plasmid

#45507

Depositor

Thomas Gelehrter

Article

Heaton et al J Biol Chem. 2001 Feb 2;276(5):3341-7. E

Insert

PAI-RBP v3 (SERBP1 Rat, Human)

Tags

GST tag

Expression

Mammalian

Available Since

June 20, 2013

Availability

Academic Institutions and Nonprofits only
pCELF PAI-RBP v1

Plasmid

#45506

Depositor

Thomas Gelehrter

Article

Heaton et al J Biol Chem. 2001 Feb 2;276(5):3341-7. E

Insert

PAI-RBP v1 (SERBP1 Rat, Human)

Tags

GST tag

Expression

Mammalian

Available Since

June 20, 2013

Availability

Academic Institutions and Nonprofits only
pCELF PAI-RBP v2

Plasmid

#45505

Depositor

Thomas Gelehrter

Article

Heaton et al J Biol Chem. 2001 Feb 2;276(5):3341-7. E

Insert

PAI-RBP v2 (SERBP1 Rat, Human)

Tags

GST tag

Expression

Mammalian

Available Since

June 20, 2013

Availability

Academic Institutions and Nonprofits only
FLAG-Cerulean-UCR1

Plasmid

#67578

Purpose

Expresses the UCR1 region of PDE4D3 in mammalian cells

Depositor

Graeme Carnegie

Article

Wang et al Cell Signal. 2015 May;27(5):908-22. doi:

Insert

Upstream conserved region 1 of phosphodiesterase 4D3 (PDE4D Human)

Tags

Cerulean and FLAG

Expression

Mammalian

Mutation

Amino acid residues 17-136 of PDE4D3

Available Since

Sept. 14, 2015

Availability

Academic Institutions and Nonprofits only