We narrowed to 9,883 results for: Plat

1. His-DERA

   Plasmid

   #203225

   Purpose

   For protein expression. E. coli 2-deoxy-D-ribose-5-phosphate aldolase. Converts dR5P to G3P and acetaldehyde. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.

   Depositor

   Vern Schramm

   Article

   Wagner et al Anal Biochem. 2023 Jul 1;672:115171. doi

   Insert

   deoxyribose aldolase
   

   Use

   Tags

   N-terminal TEV cleavable 6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1 5HT2A_NB2_1

   Plasmid

   #127690

   Purpose

   Plasmid contains a positive allosteric modulating nanobody the binds to the cytoplasmic surface of 5HT2A.

   Depositor

   Bryan Roth, Justin English

   Article

   English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

   Insert

   5HT2A_NB2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS2_hMGFP-60A

   Plasmid

   #200393

   Purpose

   A plasmid IVT template containing a humanized Monster Green Fluorescent Protein (hMGFP) CDS followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.

   Depositor

   Xiao Wang

   Article

   Aditham et al ACS Chem Biol. 2022 Dec 16;17(12):3352-3

   Insert

   monster GFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. IZ501: pMVP/Lenti-DEST

   Plasmid

   #121848

   Purpose

   pMVP destination vector, empty Lentivirus vector backbone

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Lentiviral; Pmvp gateway destination vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJAT57

   Plasmid

   #204303

   Purpose

   Expresses Cas9 under UAS control. HDR integrant markers with DsRed expressed in flight muscles.

   Depositor

   David Stern

   Article

   Stern et al bioRxiv 2023.06.17.545412

   Insert

   MHC-DsRed
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   MHC

   Available since

   Aug. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBbdCas9S_P0526-sgRNAmreB

   Plasmid

   #149654

   Purpose

   all-in-one CRISPRi vector for targeting B. burgdorferi mreB

   Depositor

   Christine Jacobs-Wagner

   Article

   Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

   Insert

   dCas9, lacI, sgRNAmreB
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PflaB, PpQE30, P0526

   Available since

   Oct. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. CCR5-donor-MCS

   Plasmid

   #192269

   Purpose

   Donor template for CRISPR Genome Editing

   Depositor

   Shantanu Chowdhury

   Article

   Sharma et al Cell Rep. 2021 May 18;35(7):109154. doi:

   Insert

   MCS within donor template
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. ArcLight-S249

   Plasmid

   #36855

   Depositor

   Vincent Pieribone

   Article

   Jin et al Neuron. 2012 Sep 6;75(5):779-85.

   Insert

   fluorescent protein voltage sensor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Ci-VSP contains R217Q mutation; super ecliptic pH…

   Promoter

   simian CMV

   Available since

   July 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTREX GPR68_NB2_2

   Plasmid

   #127691

   Purpose

   Plasmid contains a nanobody that directly interacts with the cytoplasmic surface of GPR68.

   Depositor

   Bryan Roth, Justin English

   Article

   English et al Cell. 2019 Jul 2. pii: S0092-8674(19)306

   Insert

   GPR68_NB2_2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBD-pZ-FLAG

    Plasmid

    #183148

    Purpose

    Yeast-two-hybrid BD vector with pZ promoter (activated by ZPM)

    Depositor

    Assaf Mosquna

    Article

    Feuer et al ACS Synth Biol. 2022 Jul 27. doi: 10.102

    Insert

    GAL4 BD
    

    Use

    Yeast two-hybrid

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSb_init containing anti SARS-CoV-2_RBD sybody Sb#68

    Plasmid

    #153527

    Purpose

    Expression of the anti SARS-CoV-2_RBD sybody Sb#68

    Depositor

    Markus Seeger

    Article

    Walter et al bioRxiv 2020.04.16.045419

    Insert

    Sb#68
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD

    Available since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMaCTag-Z22

    Plasmid

    #120065

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with SNAP

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    SNAP
    

    Use

    Pcr template

    Tags

    SNAP

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-tagRFP-NLS-HA-Bxb1

    Plasmid

    #65625

    Purpose

    Vector for cotranslational expression of tagRFP-Bxb1

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    Bxb1 integrase
    

    Use

    Tags

    HA, SV40 NLS, and tagRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIVT-dCas9-mCherry

    Plasmid

    #174443

    Purpose

    in vitro transcription template for dCas9-mCherry

    Depositor

    Michael Lampson

    Article

    Kumon et al Cell. 2021 Sep 16;184(19):4904-4918.e11.

    Insert

    Cas9 Endonuclease Dead
    

    Use

    In vitro transcription template

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    T7 SP6

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. PB_TRE_Z17-PDGFR_EF1a_mCherry

    Plasmid

    #186303

    Purpose

    Plasmid for inducible mammalian cell expression of the Z17 helixCAM and constitutive expression of an identifying fluorescent protein (mCherry). Flanked with PiggyBac sites for easy line construction.

    Depositor

    George Church

    Article

    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

    Inserts

    Z17-PDGFR
    mCherry
    

    Use

    Tags

    HA, myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. Col1a-3'probe

    Plasmid

    #20731

    Depositor

    Rudolf Jaenisch

    Article

    Beard et al Genesis. 2006 Jan . 44(1):23-8.

    Insert

    Col1a-3'probe (Col1a1 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMaCTag-P26

    Plasmid

    #120037

    Purpose

    Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mAID-Clover

    Depositor

    Michael Knop

    Article

    Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    mAID-Clover
    

    Use

    Pcr template

    Tags

    mAID-Clover

    Expression

    Mutation

    Promoter

    None

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. Accessory plasmid 2

    Plasmid

    #128571

    Purpose

    for cloning identified individual SPECS

    Depositor

    Timothy Lu

    Article

    Wu et al Nat Commun. 2019 Jun 28;10(1):2880. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    TagRFP

    Expression

    Mutation

    Promoter

    Available since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. CL7-LbCas12aD156R

    Plasmid

    #164659

    Purpose

    Bacterial expression of mutated LbCas12aD156R using CL7/Im7

    Depositor

    Piyush Jain

    Article

    Nguyen et al Methods. 2021 Feb 9. pii: S1046-2023(21)

    Insert

    LbCas12a-D156R
    

    Use

    Tags

    Thioredoxin, CL7, SUMO

    Expression

    Bacterial

    Mutation

    changed Aspartic Acid 156 to Arginine

    Promoter

    T7

    Available since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. modRNAc0-Cas9

    Plasmid

    #170180

    Purpose

    IVT template plasmid for making modified Cas9 RNA

    Depositor

    Xiaojun Lian

    Article

    Haideri et al Cell Rep Methods. 2022 Sep 7;2(9):100290

    Insert

    Cas9
    

    Use

    Ivt template plasmid

    Tags

    Expression

    Mutation

    Promoter

    T7 promoter

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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