We narrowed to 11,540 results for: Vars;

1. pCA_e-2

   Plasmid

   #202097

   Purpose

   Receiver cloning vector for even-2 level constructs. Contains lacZ for blue/white screening.

   Depositor

   Christopher Dupont

   Article

   Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   None

   Promoter

   Available Since

   Aug. 23, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. mTFP1-Lifeact-7

   Plasmid

   #54749

   Purpose

   Localization: Actin, Excitation: 462, Emission: 492

   Depositor

   Robert Campbell, Michael Davidson

   Article

   Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

   Type

   Empty backbone

   Use

   Tags

   mTFP1

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   June 20, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pJYDNgp

   Plasmid

   #162453

   Purpose

   Positive control plasmid for pJYDNg carrying the eUnaG2 bilirubin dependent fluorescent protein fused to the C-terminus of Aga2p protein. This plasmid contains a long 2G linker.

   Depositor

   Gideon Schreiber

   Article

   Zahradník et al bioRxiv Preprint 2020

   Insert

   Aga2p-2Glinker-eUnaG2 (AGA2 )
   

   Use

   Shuttle vector bacteria/yeast

   Tags

   HA nad myc and eUnaG2 fluorescent protein

   Expression

   Mutation

   Promoter

   GAL1

   Available Since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKSB-609 U6-sgRNA1 ATCC GGAA

   Plasmid

   #173671

   Purpose

   To clone and express a gRNA in Anopheles

   Depositor

   Eric Marois

   Article

   Klug et al PLoS Pathog. 2022 Oct 12;18(10):e1010881

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. p1.1

   Plasmid

   #162737

   Purpose

   Protein expression in CHO cells

   Depositor

   Ivan Vorobiev

   Article

   Orlova et al BMC Biotechnol. 2014 Jun 14;14:56. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

   Available Since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a_speG_Cterm_His

   Plasmid

   #220483

   Purpose

   heterologous expression for protein purification

   Depositor

   Emily Balskus

   Article

   Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

   Insert

   speG
   

   Use

   Tags

   6xHs

   Expression

   Bacterial

   Mutation

   none

   Promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSADdeltaG-ChR2-mCherry

   Plasmid

   #32646

   Purpose

   Rabies vector pSADΔG expressing ChR2-mCherry for optogenetic excitation

   Depositor

   Edward Callaway

   Article

   Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

   Insert

   ChR2
   

   Use

   Rabies virus

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 26, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pZH514

   Plasmid

   #102667

   Purpose

   Constitutive expression of GFPmut2; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

   Depositor

   Zach Hensel

   Article

   Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

   Insert

   GFPmut2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pZH516

   Plasmid

   #102669

   Purpose

   Constitutive expression of GFPmut2; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

   Depositor

   Zach Hensel

   Article

   Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

   Insert

   GFPmut2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a_PA4114_Cterm_His

    Plasmid

    #220484

    Purpose

    heterologous expression of PA4114 for protein purification

    Depositor

    Emily Balskus

    Article

    Mayers et al Cell. 2024 Jun 11:S0092-8674(24)00579-8.

    Insert

    PA4114
    

    Use

    Tags

    6xHs

    Expression

    Bacterial

    Mutation

    none

    Promoter

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX165-Sniper-Cas9

    Plasmid

    #140560

    Purpose

    Expresses Sniper Cas9 in mammalian cells

    Depositor

    Feng Zhang

    Article

    Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mutation

    F539S M763I K890N

    Promoter

    Available Since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKSB-610 U6-sgRNA2 GGAA AGAG

    Plasmid

    #173672

    Purpose

    To clone and express a gRNA in Anopheles

    Depositor

    Eric Marois

    Article

    Klug et al PLoS Pathog. 2022 Oct 12;18(10):e1010881

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pX165-Cas9-HF1

    Plasmid

    #140565

    Purpose

    Expresses Cas9-HF1 in mammalian cells

    Depositor

    Feng Zhang

    Article

    Schmid-Burgk et al Mol Cell. 2020 Mar 17. pii: S1097-2765(2

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mutation

    N497A R661A Q695A Q926A

    Promoter

    Available Since

    May 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJY3919

    Plasmid

    #113332

    Purpose

    Hermes transposable plasmid; TIR-flanked LEU2, recoded transposase driven by TEF1 promoter

    Depositor

    Suzanne Sandmeyer

    Article

    Patterson et al Metab Eng. 2018 May 22;48:184-196. doi:

    Inserts

    beta-isopropyl malate dehydrogenase
    codon-optimized Hermes transposase
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBAD/HisD-TagRFP675

    Plasmid

    #44274

    Depositor

    Vladislav Verkhusha

    Article

    Piatkevich et al Sci Rep. 2013;3:1847. doi: 10.1038/srep0

    Insert

    TagRFP675
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    M41Q/F80W/S143N/L147M/S158N/D159Y/N173S

    Promoter

    pBAD

    Available Since

    May 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-HA-MAVS-M142A(KaganF61)

    Plasmid

    #51999

    Purpose

    Expresses the human MAVS CDS containing the point mutation M142A and an N-terminal HA tag "strong translational context"

    Depositor

    Jonathan Kagan

    Article

    Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

    Insert

    MAVS
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Met 142 to Ala

    Promoter

    Available Since

    April 14, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pCMV5 MYC-Smurf2 (FF29/30A) WT

    Plasmid

    #24604

    Depositor

    Jeff Wrana

    Article

    Ogunjimi et al J Biol Chem. 2010 Feb 26. 285(9):6308-15

    Insert

    Smurf2 (SMURF2 Human)
    

    Use

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    F29A, F30A

    Promoter

    Available Since

    April 30, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. VZV ORF47

    Plasmid

    #26698

    Depositor

    Robert Kalejta

    Article

    Kuny et al PLoS Pathog. 2010 . 6(9):.

    Insert

    ORF47 (ORF47 Varicella Zoster Virus (HHV-3))
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    A 3B3 epitope tag (DQRQYGDVFKGD) from the VZV gB …

    Promoter

    Available Since

    Nov. 2, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pRDA_450

    Plasmid

    #179094

    Purpose

    nuclease Cas expression

    Depositor

    David Root, John Doench

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    SpRY
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLX_313-EGFP

    Plasmid

    #158580

    Purpose

    Lentiviral expression of EGFP

    Depositor

    David Root, John Doench

    Article

    Hanna et al Cell. 2021 Feb 18;184(4):1064-1080.e20.

    Insert

    EGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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