We narrowed to 14,273 results for: cas9

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pSaCas9-1xms2-2x3’UTR

Plasmid

#122946

Purpose

For expressing SaCas9 mRNA with one copy of MS2 aptamer and 2 copies of HBB 3' UTR

Depositor

Baisong Lu

Article

Lu et al Nucleic Acids Res. 2019 Feb 13. pii: 531

Insert

SaCas9-MS2-HBB 3' UTR

Use

AAV

Expression

Mammalian

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-xCas9RA-P2A-Puro

Plasmid

#110871

Purpose

Lentiviral vector for constitutive expression of xCas9(3.7)RA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

xCas9(3.7)RA

Use

Lentiviral

Tags

FLAG

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pRDA_256

Plasmid

#158581

Purpose

Lentiviral expression of BE3.9max and customizable gRNA

Depositor

John Doench, David Root

Article

Hanna et al Cell. 2021 Feb 18;184(4):1064-1080.e20.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Mutation

Cas9 D10A

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-ABE7.8

Plasmid

#102917

Purpose

expresses ABE7.8 in mammalian cells

Depositor

David Liu

Article

Gaudelli et al Nature. 2017 Nov 23;551(7681):464-471. d

Insert

ABE7.8

Tags

NLS

Expression

Mammalian

Mutation

see manuscript

Promoter

CMV

Available Since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-T2A-HygR

Plasmid

#118153

Purpose

SpCas9 expression vector

Depositor

Jorge Ferrer

Article

Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

Insert

hSpCas9-T2A-HygR

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Promoter

CAG and U6

Available Since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

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pDIV069

Plasmid

#247966

Purpose

CRISPR-Cas9 vector containing a 20 nt protospacer sequence to target the IS1 locus where the DIVERSIFY gene landing platform is integrated in Aspergillus nidulans. pyrG marker

Depositor

Uffe Mortensen

Insert

Cas9-AMA1-IS1-gRNA-pyrG-ori-AmpR

Use

CRISPR

Mutation

none

Available Since

March 16, 2026

Availability

Academic Institutions and Nonprofits only

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pCas9/VRQR-sgRNA (BbsI)

Plasmid

#129725

Purpose

Expressing SpCas9/VRQR mutant and sgRNA for target sequence with NGA PAM

Depositor

Elina Ikonen

Article

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-dCas9-dMQ1-EGFP

Plasmid

#89637

Purpose

Targeted CpG methylation

Depositor

Margaret Goodell

Article

Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

Insert

site-specific DNA-methyltransferase SssI

Use

CRISPR

Tags

3*FLAG, 6*His, and T2A-EGFP

Expression

Mammalian

Mutation

C141S, TGC-->TCC; S317A, AGC-->GCC

Promoter

CMV

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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HEK4-53bp-tag-insertion-pegRNA

Plasmid

#227673

Purpose

PegRNA for 53 bp insertion

Depositor

Scot Wolfe

Article

Liu et al Nat Biotechnol. 2024 Sep 25. doi: 10.103

Insert

SpCas9 sgRNA scaffold (EPHA3 Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Oct. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pTox-EGFPsite1_NGG-PAM (BPK740)

Plasmid

#181746

Purpose

p11-lacY-wtx1 derivative toxic plasmid encoding an SpCas9 target site for EGFP site 1 with an NGG PAM

Depositor

Benjamin Kleinstiver

Article

Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

Insert

ccdB toxin gene and SpCas9 target site (EGFP site 1 NGG PAM)

Use

Selection plasmid

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pSH231-EFS-Cas9-BlastR

Plasmid

#115146

Purpose

Safe harbor site 231 knock-in vector with Cas9-BlastR expression cassette

Depositor

Raymond Monnat, Michael Phelps

Article

Pellenz et al Hum Gene Ther. 2019 Jul;30(7):814-828. d

Insert

Cas9-T2A-BlastR

Use

CRISPR

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pdestMB14_sur5p-dCas9-VP64

Plasmid

#177788

Purpose

Overexpression of dCas9-VP64 in C. elegans

Depositor

Michael Ristow

Article

Fischer et al J Biol Chem. 2022 May 27:102085. doi: 10

Insert

dCas9-VP64

Expression

Worm

Available Since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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p221z-CAS9p-t35s

Plasmid

#118385

Purpose

Entry clone containing CAS9p and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway system

Depositor

Ari Pekka Mähönen

Article

Wang et al Nat Plants. 2020 Jun 29. pii: 10.1038/s4

Insert

CAS9p

Use

CRISPR

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgGrin1

Plasmid

#124852

Purpose

Mutagenesis of Grin1

Depositor

Larry Zweifel

Article

Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

Insert

Grin1 (Grin1 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pDGE78

Plasmid

#80580

Purpose

recipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, nptII

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Inserts

pnos:nptII-tmos
pPcUbi:Cas9 D10A-tnos
BsaI-ccdB_CmR-BsaI

Use

CRISPR

Expression

Plant

Mutation

D10A

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-dCas9-MQ1(WT)-EGFP

Plasmid

#89633

Purpose

Targeted CpG methylation

Depositor

Margaret Goodell

Article

Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

Insert

site-specific DNA-methyltransferase SssI

Use

CRISPR

Tags

3*FLAG and 6*His

Expression

Mammalian

Promoter

CMV

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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eSpCas9(1.1)_No_FLAG_ATP1A1_G3

Plasmid

#86611

Purpose

Expresses the ATP1A1 G3 sgRNA in combination with FLAGless eSpCas9(1.1) to target ATP1A1 intron 4. Px330-like plasmid

Depositor

Yannick Doyon

Article

Agudelo et al Nat Methods. 2017 Apr 17. doi: 10.1038/n

Insert

ATP1A1 G3 sgRNA + FLAGless enhanced specificity Cas9 (1.1)

Use

CRISPR; Co-selection via hdr using ouabain

Expression

Mammalian

Mutation

K848A, K1003A, & R1060A

Promoter

CBh

Available Since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pJCHph

Plasmid

#185863

Purpose

The CRISPR/Cas9-and-gRNA-expressing plasmid with a hygromycin B-resistent selectable marker

Depositor

Claudia Vickers

Article

Peng et al Nat Commun. 2022 May 24;13(1):2895. doi:

Insert

PTDH3-Cas9(N); PSNR52-srRNA-TSUP4; HphMX6

Expression

Yeast

Mutation

None

Available Since

Aug. 31, 2022

Availability

Academic Institutions and Nonprofits only

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iPEmax-WT

Plasmid

#239385

Purpose

For inverse prime editor using Cas9-R221K-N394K in HEK294T cells

Depositor

Caixia Gao

Article

Liang et al Nat Commun. 2025 May 31;16(1):5057. doi:

Insert

Cas9-R221K-N393K, M-MLV-RT

Use

CRISPR

Tags

BPNLS and SV40 NLS, c-myc NLS

Expression

Mammalian

Mutation

R221K, N394K in Cas9

Promoter

CMV

Available Since

Aug. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-PE2-SpRY

Plasmid

#159979

Purpose

Prime editing in mammalian cells

Depositor

Yongsub Kim

Article

Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

Insert

PE2-SpRY

Tags

BpSV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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