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GGE021

Plasmid

#120778

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for junction of position2-3. When using the GG platform for expressing only two gene (T2-3_Lip2)

Depositor

Tristan Rossignol

Publication

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

T2-3_Lip2

Use

Cloning/assembly plasmid

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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GGE002

Plasmid

#120739

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position1 (P1_GAPdh)

Depositor

Tristan Rossignol

Publication

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

P1_GAPdh

Use

Cloning/assembly plasmid

Available since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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GGE015

Plasmid

#120770

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for position 2 (T2_Lip2)

Depositor

Tristan Rossignol

Publication

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

T2_Lip2

Use

Cloning/assembly plasmid

Available since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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GGE177

Plasmid

#120766

Purpose

Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Terminator of gene expression for position 1 (T1_Xpr2)

Depositor

Tristan Rossignol

Publication

Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

Insert

T1_Xpr2

Use

Cloning/assembly plasmid

Available since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pFH22

Plasmid

#125808

Purpose

Level0 xCas9 3.7

Depositor

Vladimir Nekrasov

Publication

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

xCas9 3.7

Use

CRISPR and Synthetic Biology

Expression

Plant

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pVL93 - VP16

Plasmid

#116036

Purpose

entry vector for GreenGate cloning method, D module

Depositor

Thomas Greb

Publication

Schurholz et al Plant Physiol. 2018 Jul 19. pii: pp.18.0

Insert

VP16

Use

Unspecified

Available since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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YPRCΔ15α1

Plasmid

#101712

Purpose

GoldenBraid vector pDGB2α1, adapted for S. cerevisiae genome integration by flanking the GB cassette with recombination arms of homology for integration at the YPRCΔ15 solo LTR loci

Depositor

Elena Caro Bernat

Publication

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Type

Empty backbone

Use

Yeast expression

Available since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pTet02

Plasmid

#78284

Purpose

mRFP1 expressed behind an ATc-inducible construct

Depositor

Tom Ellis

Publication

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

Tet inducible mRFP1

Available since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pUPD3_FULLPRM_tetR_Ptet

Plasmid

#243913

Purpose

GoldenBraid2.0 Tetracycline/Anhydrotetracycline-Inducible promoter, including ribosome binding site.

Depositor

Christophe Herman

Publication

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

tetR and PtetA

Expression

Bacterial

Available since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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EC67843

Plasmid

#209513

Purpose

Cloning in complete guide expression cassettes, with Cas12a (D156R)

Depositor

Wendy Harwood, Cristobal Uauy

Publication

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Type

Empty backbone

Use

CRISPR

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMIN-0

Plasmid

#197782

Purpose

Level 2 destination vector: minimal (non-T-DNA) backbone

Depositor

Daniel Voytas

Publication

Chamness et al Plant Genome. 2023 Mar 9:e20312. doi: 10

Type

Empty backbone

Use

Unspecified

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pMC-X4

Plasmid

#176154

Purpose

preassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site X-4

Depositor

Florian David

Publication

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Type

Empty backbone

Expression

Yeast

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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MTK6_016

Plasmid

#124058

Purpose

Encodes the extended Woodchuck Hepatitis Virus (WHP) Posttranscriptional Regulatory Element (WPRE) to be used in the MTK system

Depositor

Hana El-Samad

Publication

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

WPRE (extended)

Expression

Mammalian

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK5_008

Plasmid

#123852

Purpose

Encodes ConRE' and the chicken hypersensitivity site 4 insulator as a Type 5 part to be used in the MTK system

Depositor

Hana El-Samad

Publication

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector RE'-2xHS4

Expression

Mammalian

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK4a_020

Plasmid

#123833

Purpose

Encodes the d4 PEST tag as a Type 4a part to be used in the MTK system

Depositor

Hana El-Samad

Publication

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

d4PEST tag

Expression

Mammalian

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_027

Plasmid

#123689

Purpose

Encodes ConL5 and human E4 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Publication

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L5-E4 insulator

Expression

Mammalian

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_028

Plasmid

#123690

Purpose

Encodes ConL6 and human F1 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Publication

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L6-F1 insulator

Expression

Mammalian

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK1_029

Plasmid

#123691

Purpose

Encodes ConL7 and human B2 insulator as a Type 1 part to be used in the MTK system

Depositor

Hana El-Samad

Publication

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Connector L7-B2 insulator

Expression

Mammalian

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pYTK075

Plasmid

#65182

Purpose

Encodes LEU2 as a Type 6 part to be used in the Dueber YTK system

Depositor

John Dueber

Publication

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

LEU2

Expression

Bacterial

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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pYTK053

Plasmid

#65160

Purpose

Encodes tADH1 as a Type 4 part to be used in the Dueber YTK system

Depositor

John Dueber

Publication

Lee et al ACS Synth Biol. 2015 Apr 14.

Insert

tADH1

Expression

Bacterial

Available since

June 16, 2015

Availability

Academic Institutions and Nonprofits only

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