167,318 results

1. pUltra-Plus-H2B-mTagBFP2

   Plasmid

   #161790

   Purpose

   3rd generation lentiviral bicistronic vector for mammalian expression of H2B-mTagBFP2 and one gene of interest

   Depositor

   Jorge Luis Arias-Arias

   Insert

   H2B-mTagBFP2
   

   Use

   Lentiviral; Bicistronic

   Tags

   mTagBFP2

   Expression

   Mammalian

   Mutation

   I174A in the mTagBFP2 protein according to PLoS O…

   Promoter

   UbC and UbC promoter

   Available Since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA HA HP1 gamma

   Plasmid

   #24080

   Depositor

   Naoko Tanese

   Article

   Vassallo et al Proc Natl Acad Sci U S A. 2002 Apr 30. 9

   Insert

   HP1 gamma (CBX3 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Oct. 27, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. mNeonGreen-peroxi

   Plasmid

   #137806

   Purpose

   Visualization of peroxisomes

   Depositor

   Dorus Gadella

   Article

   Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

   Insert

   SKL
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. lentiCRISPR.v2.TetON_Cas13d_U6_DR

   Plasmid

   #196727

   Purpose

   Sortable and selectable, Tet-inducible RfxCas13d with guide cassette (one vector system). Inducible knock-down.

   Depositor

   Bernhard Schmierer

   Insert

   RfxCas13d-T2A-miRFP670nano
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Promoter

   TRE

   Available Since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pScPPX2

   Plasmid

   #112877

   Purpose

   Expresses His-tagged yeast exopolyphosphatase for purification from E. coli

   Depositor

   Michael Gray, Ursula Jakob

   Article

   Gray et al Mol Cell. 2014 Mar 6;53(5):689-99. doi:

   Insert

   PPX1 (PPX1 Budding Yeast)
   

   Tags

   6X His tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. ACBE-Q

   Plasmid

   #204608

   Purpose

   Mammalian expression of high-accuracy adenine-to-cytosine base editor ACBE-Q

   Depositor

   Dali Li

   Article

   Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103

   Insert

   bpNLS-N terminal of nSpCas9-linker-mAAG (R165E/Y179F)-TadA-8e (N108Q)-linker-C terminal of nSpCas9-bpNLS-P2A-GFP
   

   Use

   CRISPR

   Tags

   6*His

   Expression

   Mammalian

   Mutation

   TadA-8e (N108Q); mAAG (R165E/Y179F); S. pyogenes …

   Available Since

   Aug. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. ptfLC3

   Plasmid

   #21074

   Purpose

   Mammalian expression of rat LC3 fused to mRFP and EGFP

   Depositor

   Tamotsu Yoshimori

   Article

   Kimura et al Autophagy. 2007;3(5):452-60.

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Tags

   EGFP and mRFP

   Expression

   Mammalian

   Available Since

   May 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. FUW-M2rtTA

   Plasmid

   #20342

   Purpose

   Lentiviral plasmid expressing the reverse tetracycline transactivator

   Depositor

   Rudolf Jaenisch

   Article

   Hockemeyer et al Cell Stem Cell. 2008 Sep 11. 3(3):346-53

   Insert

   M2rtTA
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. Flag-TurboID-NES_pLX304

   Plasmid

   #202008

   Purpose

   expresses TurboID in the mammalian cytosol, lentiviral vector

   Depositor

   Alice Ting

   Article

   Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

   Insert

   Flag-TurboID-NES
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR_HNF1A-RE_tBFP_PGK_mCitrine

    Plasmid

    #188387

    Purpose

    Lentiviral vector - tBFP reporter for HNF1A-based SNIPRs with a constitutive mCitrine;

    Depositor

    Kole Roybal

    Article

    Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

    Insert

    HNF1A-RE_tBFP_PGK_mCitrine
    

    Use

    Lentiviral

    Available Since

    March 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-syn-FLEX-splitTVA-EGFP-tTA (AAV1)

    Viral Prep

    #100798-AAV1

    Purpose

    Ready-to-use AAV1 particles produced from pAAV-syn-FLEX-splitTVA-EGFP-tTA (#100798). In addition to the viral particles, you will also receive purified pAAV-syn-FLEX-splitTVA-EGFP-tTA plasmid DNA. Helper virus for monosynaptic tracing with rabies virus; to be coinjected with pAAV-TREtight-mTagBFP2-B19G (Addgene#100799). These AAV preparations are suitable purity for injection into animals.

    Depositor

    Ian Wickersham

    Article

    Liu et al Nature. 2017 Aug 31;548(7669):582-587. d

    Promoter

    Syn

    Tags

    EGFP (Cre-dependent)

    Available Since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

12. pAGM47523

    Plasmid

    #153221

    Purpose

    Level 0 zCas9i vector

    Depositor

    Sylvestre Marillonnet

    Article

    Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

    Insert

    SpCas9 with introns and 2 NLS
    

    Use

    Synthetic Biology

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.4_StrepII_TEV_PP2Ac

    Plasmid

    #217477

    Purpose

    Expresses PP2Ac with a TEV cleavable N-terminal StrepII tag

    Depositor

    Wolfgang Peti

    Article

    Padi et al Nature. 2024 Jan;625(7993):195-203. doi:

    Insert

    PP2Ac
    

    Tags

    StrepII, TEV

    Expression

    Mammalian

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJ204-loxP-EF1a-tagBFP-HygR-lox2272-BGHpA-siteT9-HDR

    Plasmid

    #154827

    Purpose

    Landing pad for Cre/lox-based recombinase-mediated cassette exchange (RMCE) in CHO cells, donor plasmid for CRISPR/Cas9-mediated targeted integration in CHO cells (site T9)

    Depositor

    Lise Marie Grav

    Article

    Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

    Inserts

    TagBFP
    HygR
    ZsGreen1-DR
    

    Use

    CRISPR and Cre/Lox

    Tags

    PEST

    Expression

    Mammalian

    Promoter

    CMV, EF1a, and SV40 early promoter

    Available Since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLX_311-KRAB-dCas9

    Plasmid

    #96918

    Purpose

    for CRISPRi, lentiviral expression of KRAB-dCas9 and BlastR. Also called pXPR_121

    Depositor

    David Root, William Hahn, John Doench

    Article

    Rosenbluh et al Nat Commun. 2017 May 23;8:15403. doi: 10

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    KRAB

    Expression

    Mammalian

    Mutation

    dCas9

    Promoter

    EF1a

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti CMV/TO GFP-MDC1 (779-2)

    Plasmid

    #26285

    Depositor

    Eric Campeau

    Insert

    Mediator of DNA damage checkpoint 1 (MDC1 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Oct. 25, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CA

    Plasmid

    #69616

    Purpose

    To express gene strongly with AAV

    Depositor

    Naoshige Uchida

    Article

    Menegas et al Elife. 2015 Aug 31;4. doi: 10.7554/eLife

    Type

    Empty backbone

    Use

    AAV and Cre/Lox

    Expression

    Mammalian

    Available Since

    Oct. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pgRNA-CKB

    Plasmid

    #73501

    Purpose

    gRNA expression vector (with mKate2)

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Insert

    mKate2-T2A-Bsd
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBS594 promoterless EGFPcre

    Plasmid

    #11956

    Depositor

    Brian Sauer

    Article

    Le et al Anal Biochem. 1999 Jun 1. 270(2):334-6.

    Insert

    EGFP-cre (cre bacteriophage P1)
    

    Use

    Cre/Lox

    Expression

    Mammalian

    Available Since

    Sept. 28, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHR_Gal4UAS_dGFP_PGK_iRFP (pLZA042)

    Plasmid

    #203908

    Purpose

    Encodes dGFP downstream UAS promoter that's responsive to Gal4-VP64 activity in mammalian cells

    Depositor

    Jared Toettcher

    Article

    Zhu et al Nat Commun. 2023 Jun 2;14(1):3185. doi:

    Insert

    Gal4UAS_dGFP_PGK_iRFP
    

    Expression

    Mammalian

    Promoter

    5xUAS and PGK

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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