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p2CT-His-MBP-Lbu_C2c2_K1082A

Plasmid

#91863

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

LbuCas13a (LbuC2c2) K1082A

Tags

His6-MBP-TEV site

Expression

Bacterial

Mutation

K1082A

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pGG-PIP08

Plasmid

#196746

Purpose

Contains CIPP1 promoter

Depositor

Marc Somssich

Article

Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

Insert

CERK1-INTERACTING PROTEIN PHOSPHATASE 1 (AT1G34750 Mustard Weed)

Use

Greengate (gg) entry vector

Available Since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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Sc NybbR eRF1 (G180A)

Plasmid

#175415

Purpose

Encodes His-MBP-TEV-ybbR-G180A eRF1

Depositor

Joseph Puglisi

Article

Lawson et al Science. 2021 Aug 20;373(6557):876-882.

Insert

eRF1 (SUP45 Budding Yeast)

Tags

His-MBP-TEV and ybbR

Expression

Bacterial

Mutation

G180A

Promoter

T7

Available Since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pJCC_084 Cas12i1 D647A

Plasmid

#171671

Purpose

For bacterial expression of Cas12i1 D647A (RuvC-inhibiting mutation) with an N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

Insert

Cas12i1 D647A

Tags

10X His, TEV protease cleavage site, and maltose-…

Expression

Bacterial

Mutation

changed aspartate 647 to alanine

Available Since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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EcNusGKOW

Plasmid

#113124

Purpose

Expresses C-terminus of E. coli NusG

Depositor

James Berger

Article

Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

Insert

NusG (nusG Escherichia coli)

Tags

His6-MBP-N10-TEV

Expression

Bacterial

Mutation

Deleted residues 1-124

Promoter

T7

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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EcRfaHKOW

Plasmid

#113125

Purpose

Expresses C-terminus of E. coli RfaH

Depositor

James Berger

Article

Lawson et al Mol Cell. 2018 Sep 20;71(6):911-922.e4.

Insert

RfaH (rfaH Escherichia coli)

Tags

His6-MBP-N10-TEV

Expression

Bacterial

Mutation

Deleted residues 1-107

Promoter

T7

Available Since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_K310A

Plasmid

#91856

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

LbuCas13a (LbuC2c2) K310A

Tags

His6-MBP-TEV site

Expression

Bacterial

Mutation

K310A

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_D1078A

Plasmid

#91860

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

LbuCas13a (LbuC2c2) D1078A

Tags

His6-MBP-TEV site

Expression

Bacterial

Mutation

D1078A

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_N314A

Plasmid

#91858

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

LbuCas13a (LbuC2c2) N314A

Tags

His6-MBP-TEV site

Expression

Bacterial

Mutation

N314A

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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p2CT-His-MBP-Lbu_C2c2_K1080A

Plasmid

#91862

Purpose

Bacterial expression for Cas13a

Depositor

Jennifer Doudna

Article

East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

Insert

LbuCas13a (LbuC2c2) K1080A

Tags

His6-MBP-TEV site

Expression

Bacterial

Mutation

K1080A

Available Since

June 19, 2017

Availability

Academic Institutions and Nonprofits only

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pFOSGE_DUM1B01

Plasmid

#71089

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Clk (Clk Fly)

Use

Gateway entry vector

Available Since

Feb. 29, 2016

Availability

Academic Institutions and Nonprofits only
Wnt4 Non-STOP

Plasmid

#35892

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt4 (WNT4 Human)

Use

Entry vector

Mutation

Silent Mutation C (492) --> T

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pNW-sfGFP(SPS6)

Plasmid

#133814

Purpose

Fluorescent protein sfGFP(SPS6) variant for optimized expression in Bacillus mycoides.

Depositor

Oscar Kuipers

Article

Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

Insert

sfGFP(SPS6)

Use

E.coli/bacillus spp. shuttle vector

Expression

Bacterial

Promoter

Ppta

Available Since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pUAS-V5-miniTurboID-NES

Plasmid

#116905

Purpose

Expresses miniTurboID under Gal4/UAS control. miniTurboID is tagged at the N-terminus with V5 and a nuclear export signal (NES) at the C-terminus.

Depositor

Norbert Perrimon

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

miniTurboID

Use

Drosophila expression

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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EGFP-Rubicon

Plasmid

#221659

Purpose

Rubicon tagged N-terminally with EGFP; clone a gift from the Hurley Lab at UC Berkeley

Depositor

Erika Holzbaur

Article

Basak et al Nat Commun. 2025 Aug 9;16(1):7367. doi:

Insert

RUBCN (RUBCN Human)

Tags

EGFP

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pJCC_076 AsCas12a R1226A

Plasmid

#171669

Purpose

For bacterial expression of AsCas12a R1226A (RuvC-inhibiting mutation) with an N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

Insert

AsCas12a R1226A

Tags

10X His, TEV protease cleavage site, and maltose-…

Expression

Bacterial

Mutation

changed arginine 1226 to alanine

Available Since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pJCC_075 AsCas12a D908A

Plasmid

#171668

Purpose

For bacterial expression of AsCas12a D908A (RuvC-inactivating mutation) with an N-terminal His-MBP tag

Depositor

Jennifer Doudna

Article

Cofsky et al Elife. 2020 Jun 10;9. pii: 55143. doi: 1

Insert

AsCas12a D908A

Tags

10X His, TEV protease cleavage site, and maltose-…

Expression

Bacterial

Mutation

changed aspartate 908 to alanine

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pET29b-AK1-His

Plasmid

#124135

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Adenylate kinase (AK1 Chicken)

Tags

6x-His

Expression

Bacterial

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pUAS-V5-TurboID-NES

Plasmid

#116904

Purpose

Expresses TurboID under Gal4/UAS control. TurboID is tagged at the N-terminus with V5 and a nuclear export signal (NES) at the C-terminus.

Depositor

Norbert Perrimon

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

TurboID

Use

Drosophila expression

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pGex-3x MKK4

Plasmid

#14617

Depositor

Roger Davis

Article

Derijard et al Science. 1995 Feb 3. 267(5198):682-5.

Insert

MKK4 (MAP2K4 Human)

Tags

GST

Expression

Bacterial

Available Since

July 25, 2007

Availability

Academic Institutions and Nonprofits only