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pcDNA4/TO CD16.7-h-PKD1 FLM R4114A

Plasmid

#83461

Purpose

Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and a mutation in the first Calmodulin binding domain (503)

Depositor

Thomas Weimbs

Article

Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

Insert

Polycystin-1 (PKD1 Human)

Tags

CD16.7

Expression

Mammalian

Mutation

Amino-acids 4078-4303, R4113A

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-Claspin-MΔN

Plasmid

#31624

Depositor

Aziz Sancar

Article

Lindsey-Boltz et al J Biol Chem. 2009 Nov 27. 284(48):33107-

Insert

Claspin (CLSPN Human)

Tags

GST

Expression

Bacterial

Mutation

aa 899-1206 only

Available Since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA1-GATA4-opposite orientation

Plasmid

#51928

Purpose

for T7 directed antisense production of mouse GATA4

Depositor

Jeffery Molkentin

Article

Liang et al J Biol Chem. 2001 Aug 10;276(32):30245-5

Insert

GATA4 reverse orientation (Gata4 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

May 28, 2014

Availability

Academic Institutions and Nonprofits only
pQE60-mPU.1-ETS

Plasmid

#85733

Purpose

Expresses DNA-binding (ETS) domain of murine PU.1, residues 167-272, in M15 cells already pre-transformed with 2nd plasmid, pREP-4

Depositor

Gregory Poon

Article

Poon et al J Biol Chem. 2012 May 25;287(22):18297-3

Insert

ETS domain of transcription factor PU.1 (Spi1 Mouse)

Tags

6xHis tag

Expression

Bacterial

Available Since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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CMV epArcLight

Plasmid

#85803

Purpose

genetically-encoded fluorescent voltage indicator

Depositor

Vincent Pieribone

Article

Platisa et al ACS Chem Neurosci. 2017 Jan 3. doi: 10.1

Insert

epArcLight

Expression

Mammalian

Mutation

Ci-VSP contains R217Q mutation; ecliptic pHluorin…

Promoter

CMV

Available Since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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HOXA9 (murine) FLAG pRC/CMV

Plasmid

#8514

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

Insert

HOXA9 (Hoxa9 Mouse)

Tags

FLAG and His

Expression

Mammalian

Mutation

FLAG epitope tag replaces T7 tag in the pET back…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pET23a_mod_RBPMS_FL

Plasmid

#59393

Purpose

Plasmid for bacterial overexpression of RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Tags

6xHis, FLAG, and HA

Expression

Bacterial

Promoter

T7

Available Since

Feb. 10, 2015

Availability

Academic Institutions and Nonprofits only

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hisSUMO-Gli1

Plasmid

#87225

Purpose

Express hisSUMO tagged DNA binding domain of Gli1(mouse) in E. coli

Depositor

Gary Stormo

Article

Zuo et al Sci Adv. 2017 Nov 17;3(11):eaao1799. doi

Insert

GLI1 (Gli1 Mouse)

Tags

hisSUMO

Expression

Bacterial

Promoter

T7

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-p160(1-200)

Plasmid

#43

Depositor

Bruce Spiegelman

Article

Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

Insert

p160 myb binding protein (Mybbp1a Mouse)

Tags

myc

Expression

Mammalian

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
LB360 (Bcl-2 from -1287 to -1337)

Plasmid

#15242

Depositor

Linda Boxer

Article

Wilson et al Mol Cell Biol. 1996 Oct . 16(10):5546-56

Insert

B-cell lymphoma/leukemia-2 (BCL2 Human)

Use

Luciferase

Tags

Luciferase

Expression

Bacterial

Mutation

Bcl-2 fragment from -1287 to -1337 with P1

Available Since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
pRS306Sup35R1

Plasmid

#17715

Depositor

Susan Lindquist

Article

Dong et al J Biol Chem. 2007 Nov 23. 282(47):34204-

Insert

pRS306Sup35R1 (SUP35 Budding Yeast)

Expression

Yeast

Mutation

An pRS306 based integrating plasmid designed for …

Available Since

April 4, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3myc-PR-LBD

Plasmid

#89113

Purpose

mammalian expression of human progesterone receptor ligand binding domain amino acid residues 632-933 with N-terminal myc tag

Depositor

Elizabeth Wilson

Article

Liu et al J Biol Chem. 2011 Aug 26;286(34):29951-6

Insert

human progesterone receptor ligand binding domain (PGR Human)

Tags

myc

Expression

Mammalian

Mutation

contains Ligand Binding Domain (632-933)

Promoter

CMV

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only
pETDUET1-hUCH37 (ISF2)-NFRKB(39-156)

Plasmid

#61938

Purpose

Co-expression of hUCH37 (ISF2) and hNFRKB (39-156) in E.coli

Depositor

Chris Hill

Article

VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

Inserts

hUCH37 (ISF2) (UCHL5 Human)
hNFRKB (39-156) (NFRKB Human)

Tags

6x HIS

Expression

Bacterial

Mutation

Construct ends at residue 156, residues 2-38 dele…

Promoter

T7

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only
pET151-GFP-PP-RPN2 (925-953)

Plasmid

#73744

Purpose

Expression of GFP-PP-RPN2 (925-953) fusion in E.coli

Depositor

Chris Hill

Article

VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

Insert

RPN2 (RPN2 Human)

Tags

6x His and GFP

Expression

Bacterial

Mutation

Construct possesses only residues 932-953 of RPN2

Promoter

T7

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only
pGEX-4T1-Claspin-BPII

Plasmid

#31632

Depositor

Aziz Sancar

Article

Lindsey-Boltz et al J Biol Chem. 2009 Nov 27. 284(48):33107-

Insert

Claspin (CLSPN Human)

Tags

GST

Expression

Bacterial

Mutation

aa 492-622 of claspin

Available Since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pTet02

Plasmid

#78284

Purpose

mRFP1 expressed behind an ATc-inducible construct

Depositor

Tom Ellis

Article

Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

Insert

Tet inducible mRFP1

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pWJPrP1

Plasmid

#22109

Depositor

Susan Lindquist

Article

Jackson et al Neuron. 2009 Aug 27. 63(4):438-50.

Insert

mouse PrP ORF insert (Prnp Mouse)

Use

Construction of targeting vector

Mutation

mutations at aa 108 and 111 to make 3F4 epitope (…

Available Since

Oct. 16, 2009

Availability

Academic Institutions and Nonprofits only
pENTR4-RBPMS

Plasmid

#59387

Purpose

Entry vector with RBPMS

Depositor

Thomas Tuschl

Article

Farazi et al RNA. 2014 Jul;20(7):1090-102. doi: 10.12

Insert

RBPMS (RBPMS Human)

Available Since

Feb. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pET151-GFP-PP-RPN2 (918-932)

Plasmid

#73748

Purpose

Expression of GFP-PP-RPN2 (918-932) fusion in E.coli

Depositor

Chris Hill

Article

VanderLinden et al J Biol Chem. 2017 Apr 25. pii: jbc.M117.

Insert

RPN2 (RPN2 Human)

Tags

6x His and GFP

Expression

Bacterial

Mutation

Construct possesses only residues 918-932 of RPN2

Promoter

T7

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only
FgH1tUTG_huMcl-1.2

Plasmid

#85531

Purpose

Inducible expression of guide RNA (huMcl-1.2) with fluorescent GFP reporter

Depositor

Marco Herold

Article

Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

Insert

hu Mcl-1.2

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

H1t

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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