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We narrowed to 63,812 results for: cloning

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Showing: 2521 - 2540 of 63812 results

  1. pJRH-1345 U6-B2M sgRNA Gag-Cas9 v2

    Plasmid
    #201916
    Purpose
    Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and B2M sgRNA (human U6 promoter, 5’-GAGTAGCGCGAGCACAGCTA)
    Depositor
    Jennifer Doudna
    Publication
    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Gag-Cas9 v2
    B2M sgRNA (Target-specific sequence: 5'- GAGTAGCGCGAGCACAGCTA)
    Expression
    Mammalian
    Promoter
    CAG and Human U6
    Available since
    Aug. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    201916-plasmid-map-sequence-id-403306

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  6. m6A-Tracer-NES

    Plasmid
    #159607
    Purpose
    Constitutive transient mammalian expression of m6A-Tracer protein (Kind et al. 2013) with a C-terminal HIV-1 Rev Nuclear Export Signal to reduce background due to nonspecific DNA interactions.
    Depositor
    Aaron Streets
    Publication
    Altemose et al Cell Syst. 2020 Oct 21;11(4):354-366.e9.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    m6A-Tracer-NES
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    159607-plasmid-map-sequence-id-311957

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  15. STARR-seq luciferase validation vector_ORI_neg.cont

    Plasmid
    #99315
    Purpose
    Negative control for luciferase validation vectors
    Depositor
    Alexander Stark
    Publication
    Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    neutral region; chr3: 55198740-55200041
    Use
    Luciferase; Starr-seq luciferase validation vector
    Expression
    Mammalian
    Available since
    Oct. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    99315-plasmid-map-sequence-id-193174

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  17. lentiGuide-Hygro-iRFP670

    Plasmid
    #99377
    Purpose
    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and hygromycin resistance marker with 2A iRFP670 from EF-1a promoter. 3rd generation lentiviral backbone.
    Depositor
    Kristen Brennand
    Publication
    Ho et al Stem Cell Reports. 2017 Aug 8;9(2):615-6
    Inserts
    S. pyogenes sgRNA cassette
    Hygro-P2A-iRFP670
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EF-1a and hU6
    Available since
    Aug. 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    99377-plasmid-map-sequence-id-191028

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  18. pDONR223_JAK2_WT

    Plasmid
    #81756
    Purpose
    Gateway Donor vector containing JAK2 , part of the Target Accelerator Plasmid Collection. *Please note this is not a true WT JAK2 sequence and contains a V617F mutation.
    Depositor
    Jesse Boehm, William Hahn, David Root
    Publication
    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi
    Insert
    JAK2 (JAK2 Human)
    Use
    Gateway Cloning
    Mutation
    V617F
    Promoter
    None
    Available since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    81756-plasmid-map-sequence-id-168863

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Showing: 2521 - 2540 of 63812 results