We narrowed to 17,455 results for: emb

1. pIRES:hTRAAK

   Plasmid

   #133080

   Purpose

   Mammalian expression vector. It will generate the human TRAAK channel full length

   Depositor

   Dan Minor

   Article

   Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

   Insert

   Potassium channel subfamily K member 4 (KCNK4, TRAAK) (KCNK4 Human)
   

   Expression

   Mammalian

   Available Since

   May 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. TC1332

   Plasmid

   #164874

   Purpose

   CURE-N: C to U RNA Editing, with high efficiency and low off-target effects

   Depositor

   Tian Chi

   Article

   Huang et al EMBO J. 2020 Nov 16;39(22):e104741. doi:

   Insert

   hAPOBEC3A fused to PspCas13b (APOBEC3A )
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   human apobec Y132D

   Available Since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. GFP-LiMETER-v2

   Plasmid

   #113934

   Purpose

   Also termed as OptoPBer; Express an STIM1-derived optical tether to label and interrogate ER-plasma membrane contact sites (MCS) with light; visualized by GFP

   Depositor

   Yubin Zhou

   Article

   He et al Chem Sci. 2017 Aug 1;8(8):5275-5281. doi

   Insert

   LOV2 and Stim1 polybasic domain (PB)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. TC1681

   Plasmid

   #164872

   Purpose

   CURE-C2: C to U RNA Editing, with high efficiency and high off-target effects

   Depositor

   Tian Chi

   Article

   Huang et al EMBO J. 2020 Nov 16;39(22):e104741. doi:

   Insert

   hAPOBEC3A fused to PspCas13b (APOBEC3A )
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   human apobec Y132D

   Available Since

   Feb. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLaNGo11-7

   Plasmid

   #126055

   Purpose

   A self-assembling split-GFP based Golden Gate vector to determine protein targeting to plastids in plant cell

   Depositor

   Ralf-Bernd Klösgen

   Article

   Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

   Inserts

   chloroplastic FNR transit peptide
   ccdB cassette (BsaI)
   GFP11x7 (5 aa linker)
   

   Use

   Binary

   Tags

   GFP1-10

   Expression

   Plant

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIRES2-hPAC

   Plasmid

   #129488

   Purpose

   Expresses human TMEM206

   Depositor

   Zhaozhu Qiu

   Article

   Yang et al Science. 2019 Apr 26;364(6438):395-399.

   Insert

   Transmembrane protein 206 (PACC1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOPS0379

   Plasmid

   #115510

   Purpose

   Suitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing sfGFP

   Depositor

   Philip Poole

   Article

   Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

   Insert

   Rlv3841pNifH(pOGG082) sfGFP (pOGG037) and T-pharma (pOGG003) assembled in pOGG026
   

   Expression

   Bacterial

   Mutation

   Domesticated for Golden Gate cloning

   Available Since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. PP_080_pAAV_CAG_DIO-LR-Voltron2-P2A-CheRiff-eGFP-ST (Kv2.1TS)

   Plasmid

   #230990

   Purpose

   Co-expressing membrane-localized Voltron2 and membrane-localized CheRiff.

   Depositor

   Adam Cohen

   Article

   Wong-Campos et al bioRxiv [Preprint]. 2023 May 26:2023.05.

   Insert

   Cre-dependent, membrane-localized Voltron2 and soma-localized CheRiff
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   June 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAV126

   Plasmid

   #63192

   Purpose

   pRS426 backbone, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Available Since

   Aug. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-MSP1D1delltaH4

    Plasmid

    #71713

    Purpose

    Membrane scaffold protein (MSP), helix 4 deletion

    Depositor

    Gerhard Wagner

    Article

    Hagn et al J Am Chem Soc. 2013 Feb 6;135(5):1919-25

    Insert

    Apolipoprotein A1 (APOA1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Available Since

    March 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. TC1614

    Plasmid

    #164873

    Purpose

    CURE-X: C to U RNA Editing, with low off-target effects but also lower efficiency

    Depositor

    Tian Chi

    Article

    Huang et al EMBO J. 2020 Nov 16;39(22):e104741. doi:

    Insert

    hAPOBEC3A inserted into RfxCas13d (APOBEC3A )
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    human apobec Y132D

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. mCer-4aa-VAMP2

    Plasmid

    #53233

    Purpose

    Fluorescent probe: VAMP2 (synaptobrevin2), N-terminally-tagged with monomeric Cerulean, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCit-4aa-SNAP25B for FRET.

    Depositor

    Robert Zucker

    Article

    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

    Insert

    VAMP2 (Vamp2 Rat)
    

    Tags

    4aa-linker and mCerulean

    Expression

    Mammalian

    Available Since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pMM2-hPMCA4b

    Plasmid

    #47759

    Purpose

    mammalian expression of human PMCA4b

    Depositor

    John Penniston, Emanuel Strehler

    Article

    Adamo et al Biochem J. 1992 Aug 1;285 ( Pt 3):791-7.

    Insert

    PMCA4b (ATP2B4 Human)
    

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. Sec61b-HA-mNG21-10

    Plasmid

    #141213

    Purpose

    Membrane Tethered Split Fluorescent Reporter for ER Retragrade

    Depositor

    Wayne Lencer

    Article

    Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

    Insert

    Sec61b-HA-mNG21-10
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Available Since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBTK620

    Plasmid

    #110620

    Purpose

    BTK assembled broad-host-range plasmid encoding GFP driven by CP32 promoter

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    GFP
    

    Expression

    Bacterial

    Available Since

    Jan. 22, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pBTK621

    Plasmid

    #110621

    Purpose

    BTK assembled broad-host-range plasmid encoding GFP driven by CP6 promoter

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    GFP
    

    Expression

    Bacterial

    Available Since

    Sept. 21, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pBTK619

    Plasmid

    #110619

    Purpose

    BTK assembled broad-host-range plasmid encoding GFP driven by CP12b promoter

    Depositor

    Jeffrey Barrick

    Article

    Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

    Insert

    GFP
    

    Expression

    Bacterial

    Available Since

    Aug. 10, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. pAAV.U6gLacZ.hSyn.flex.H2B.RFP

    Plasmid

    #170373

    Purpose

    Negative control guide, Cre-inducible plasmid expressing nuclear RFP.

    Depositor

    Johan Jakobsson

    Article

    Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

    Insert

    Cre inducible H2B RFP
    

    Use

    AAV

    Promoter

    human Synapsin

    Available Since

    Jan. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. mbYFPQS

    Plasmid

    #80742

    Purpose

    membrane-targeted biosensor for monitoring changes in intracellular chloride

    Depositor

    Susan Amara, Susan Ingram

    Article

    Watts et al PLoS One. 2012;7(4):e35373. doi: 10.1371

    Insert

    YFPQS
    

    Tags

    palmitoylation signal sequence of neuromodulin

    Expression

    Mammalian

    Mutation

    H148Q (CAC to CAG) and V163S (GTG to AGC).

    Promoter

    CMV

    Available Since

    Aug. 3, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pMM2-hPMCA1b

    Plasmid

    #47758

    Purpose

    mammalian expression of human PMCA1b

    Depositor

    John Penniston, Emanuel Strehler

    Article

    Adamo et al Biochem J. 1992 Aug 1;285 ( Pt 3):791-7.

    Insert

    PMCA1 (ATP2B1 Human)
    

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Sept. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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