We narrowed to 12,691 results for: lic

1. sgRNA1_Cas9-hGem_Smc4

   Plasmid

   #217667

   Purpose

   expresses Cas9-hGem and guideRNA for tagging of SMC4

   Depositor

   Daniel Gerlich

   Article

   Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

   Insert

   sgRNA1 targeting SMC4 C-terminal (SMC4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Hygro-P2A-HA-FKBP-WAPL

   Plasmid

   #217666

   Purpose

   Repair template to endogenously tag WAPL with Hygro-P2A-HA-FKBP

   Depositor

   Daniel Gerlich

   Article

   Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

   Insert

   WapI (WAPL Human)
   

   Use

   Tags

   dTag (FKBP36V protein)

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Lac

   Available since

   July 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti_CHyMErA.v2_U6(SpCas9_I-SceI)-(AsCas12a(dual-gRNA)_PacI-PacI)_PGK-puro

   Plasmid

   #189636

   Purpose

   Lentiviral expression of single SpCas9 and dual AsCas12a gRNAs for generating combinatorial CHyMErA.v2 3Cs libraries

   Depositor

   Manuel Kaulich

   Article

   Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

   Insert

   hU6 Cas9-Cas12a-Cas12a gRNA cassette, PGK puro cassette
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMMK ENTR 1 HITI-2c ACTB

   Plasmid

   #206269

   Purpose

   ENTR Vector 1 for MultiSite Gateway assembly. Encodes HITI-2c donor for ACTB C-terminal tagging (mCherry T2A Puro) in human cells

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Insert

   HITI-2c donor
   

   Use

   CRISPR; Multimate/gateway entr 1

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. anti-Calbindin scFv [L109/57] with sortase tag

   Plasmid

   #204421

   Purpose

   Mammalian Expression of anti-Calbindin scFv with a sortase tag for direct dye conjugation. Derived from hybridoma L109/57.

   Depositor

   Jeff Lichtman

   Article

   Han et al bioRxiv. 2023 Jun 8:2023.05.20.540091. d

   Insert

   Recombinant mouse scFv targeting Calbindin (Homo sapiens)
   

   Use

   Tags

   6xHis tag and Sortase tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. anti-Parvalbumin scFv [L114/81] with sortase tag

   Plasmid

   #204423

   Purpose

   Mammalian Expression of anti-Parvalbumin scFv with a sortase tag for direct dye conjugation. Derived from hybridoma L114/81.

   Depositor

   Jeff Lichtman

   Article

   Han et al bioRxiv. 2023 Jun 8:2023.05.20.540091. d

   Insert

   Recombinant mouse scFv targeting Parvalbumin (Rattus norvegicus)
   

   Use

   Tags

   6xHis tag and Sortase tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. anti-Kv1.2 scFv [K14/16] with sortase tag

   Plasmid

   #204425

   Purpose

   Mammalian Expression of anti-Kv1.2 scFv with a sortase tag for direct dye conjugation. Derived from hybridoma K14/16.

   Depositor

   Jeff Lichtman

   Article

   Han et al bioRxiv. 2023 Jun 8:2023.05.20.540091. d

   Insert

   Recombinant mouse scFv targeting Kv1.2 (Homo sapiens)
   

   Use

   Tags

   6xHis tag and Sortase tag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. Coexpressed VLP NES

   Plasmid

   #182433

   Purpose

   Yeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20 (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3)

   Depositor

   Claudia Vickers

   Article

   Cheah et al bioRxiv 2022.11.24.517869

   Inserts

   VP2C-FPPS
   deltaVP1
   VP2C-NES
   

   Use

   Synthetic Biology; Metabolic engineering

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1, GAL10, and GAL7

   Available since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Coexpressed VLP NES (M)

   Plasmid

   #182435

   Purpose

   YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20(F96W-N127W) (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al bioRxiv 2022.11.24.517869

   Inserts

   VP2C-FPPS(M)
   deltaVP1
   VP2C-NES
   

   Use

   Synthetic Biology; Metabolic engineering

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1, GAL10, and GAL7

   Available since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. Coexpressed VLP NES (G)

    Plasmid

    #182477

    Purpose

    YIp for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-ERG20(F96C) (GAL10 promoter), and VP2C-AcNES1 (GAL7 promoter). Contains uracil marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al bioRxiv 2022.11.24.517869

    Inserts

    VP2C-FPPS(G)
    deltaVP1
    VP2C-NES
    

    Use

    Synthetic Biology; Metabolic engineering

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1, GAL10, and GAL7

    Available since

    Dec. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Coexpressed Free PcPTS

    Plasmid

    #182483

    Purpose

    Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and patchoulol synthase from Pogostemon cablin (PcPTS; GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

    Inserts

    FPPS
    PTS
    

    Use

    Synthetic Biology; Metabolic engineering

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL10 and GAL7

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. Linked Free NES (AG4TGGA)2

    Plasmid

    #182488

    Purpose

    Yeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence (AG4TGGA)2 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

    Insert

    FPPS-NES
    

    Use

    Synthetic Biology; Metabolic engineering

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL10

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. Linked Free (PT)4P

    Plasmid

    #182490

    Purpose

    Yeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence PTPTPTPTP (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

    Insert

    FPPS-NES
    

    Use

    Synthetic Biology; Metabolic engineering

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL10

    Available since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDTCendoNA2

    Plasmid

    #174732

    Purpose

    Expresses DT386C-endoNA2 fusion protein for selective elimination of polysialic acid–containing cells. Truncated diphtheria toxin fused to noncatalytic endosialidase via a caspase-3/7-cleavable linker

    Depositor

    Jukka Finne

    Article

    Lehti et al Mol Cancer Ther. 2021 Jul 26. pii: 1535-

    Insert

    DT386C
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Deleted amino acids 412-560 (the receptor binding…

    Promoter

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDestTol2-actinb-kcnj1b-IRES-EGFP

    Plasmid

    #164946

    Purpose

    Tol2 construct: actinb (actb2) promoter driven zebrafish kcnj1b and EGFP

    Depositor

    GuangJun Zhang

    Article

    Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

    Insert

    kcnj1b (kcnj1b Zebrafish)
    

    Use

    Tol2 transposon destination vector

    Tags

    IRES-EGFP

    Expression

    Mutation

    Promoter

    actinb (actb2) promoter

    Available since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. GFP-Tac-STC

    Plasmid

    #162490

    Purpose

    Expresses partial length Tac (stem region, TMD and cytosolic tail) with GFP tag in mammalian cells

    Depositor

    Lei Lu

    Article

    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

    Insert

    partial length Tac (stem region, TMD and cytosolic tail) (IL2RA Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Tac is in partial length with its stem region, TM…

    Promoter

    Available since

    Jan. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pETM33_Nsp3c_SUD-C

    Plasmid

    #156471

    Purpose

    Bacterial expression of Sars-CoV2 Nsp3c_SUD-C protein with His-tag and GST-tag

    Depositor

    Ylva Ivarsson

    Article

    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

    Insert

    Nsp3c_SUD-C (ORF1ab Synthetic)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEMHE:mTREK-1_cryst

    Plasmid

    #133271

    Purpose

    X. laevis expression vector. It will generate the TREK-1 channel

    Depositor

    Dan Minor

    Article

    Lolicato et al Nature. 2017 Jul 20;547(7663):364-368. d

    Insert

    Potassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 )
    

    Use

    Oocyte expression

    Tags

    Expression

    Mutation

    aa 21-322, K84R, Q85E, T86K, I88L, A89R, Q90A, A9…

    Promoter

    Available since

    April 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPICZ-pOst1-pro-alphaf(MUT2)-E2-Crimson

    Plasmid

    #117663

    Purpose

    Test intracellular localisation of E2-Crimson and study the secretion efficiency

    Depositor

    Pau Ferrer, Benjamin Glick

    Article

    Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

    Insert

    pOst1-pro-af(MUT2)-E2-Crimson
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    This plasmid encodes the fluorescent protein E2-C…

    Promoter

    pAOX1

    Available since

    Dec. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. Human CRISPR Poison Exon Knockout Library

    Pooled Library

    #138084

    Purpose

    The human pgPoison library induces skipping of “poison” cassette exons and corresponding upstream constitutive exons by targeting 3' splice sites with paired gRNAs (pgRNAs).

    Depositor

    Robert Bradley

    Article

    Thomas et al Nat Genet. 2020 Jan;52(1):84-94. doi: 10

    Species

    Homo sapiens

    Use

    CRISPR and Lentiviral

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only