We narrowed to 18,031 results for: Mut

1. pcDNA3-5'UTR-MAVS-ORF1(KaganE49)

   Plasmid

   #52006

   Purpose

   Expresses the human MAVS CDS and contains the 5'UTR sequence with a mutation in the start codon 37 nt upstream of the FL MAVS start codon

   Depositor

   Jonathan Kagan

   Article

   Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

   Insert

   5utr-MAVS-ORF1mut
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mutation removing the start codon for ORF1

   Promoter

   Available Since

   March 24, 2014

   Availability

   Academic Institutions and Nonprofits only

2. L1-W1036L

   Plasmid

   #15488

   Depositor

   Vance Lemmon

   Article

   Cheng et al Mol Cell Neurosci. 2004 Dec . 27(4):522-

   Insert

   L1-W1036L (L1CAM Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Tryptophan 1036 changed to Leucine

   Promoter

   Available Since

   Oct. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

3. pEVOL-pylT-N346A/C348A

   Plasmid

   #127411

   Purpose

   Double mutant of wild type mmPylRS designed for incorporation of non-canonical amino acids with mono-substituted phenylalanine derivatives and tyrosinyl ethers

   Depositor

   Wenshe Liu

   Article

   Wang et al J Am Chem Soc. 2012 Feb 15;134(6):2950-3

   Insert

   Pyrrolysyl-tRNA synthetase/pyrrolysyl -tRNA pair
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N346A-C348A

   Promoter

   araBAD

   Available Since

   July 17, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. ArcLight-Q239

   Plasmid

   #36856

   Depositor

   Vincent Pieribone

   Article

   Jin et al Neuron. 2012 Sep 6;75(5):779-85.

   Insert

   fluorescent protein voltage sensor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Ci-VSP contains R217Q mutation; super ecliptic pH…

   Promoter

   simian CMV

   Available Since

   July 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. MSCV Puro SCRIB WT

   Plasmid

   #88886

   Purpose

   SCRIB WT in MSCV backbone

   Depositor

   Senthil Muthuswamy

   Article

   Zheng et al Nat Cell Biol. 2016 Nov;18(11):1244-1252

   Insert

   SCRIB (SCRIB Human)
   

   Use

   Retroviral

   Tags

   N/A

   Expression

   Mutation

   N/A

   Promoter

   MSCV promoter

   Available Since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. GFP-Ub KO.G76V

   Plasmid

   #11932

   Depositor

   Nico Dantuma

   Article

   Dantuma et al J Cell Biol. 2006 Apr 10. 173(1):19-26.

   Insert

   Ubiquitin KO.G76V (RBX1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Conjugation deficient mutant. All 7 lysines of ub…

   Promoter

   Available Since

   Aug. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCas9D10A_GFP

   Plasmid

   #44720

   Purpose

   Co-expression of human codon-optimized Cas9 (D10A) mutant nickase and GFP, plasmid optimized for expression in human pluripotent stem cells

   Depositor

   Kiran Musunuru

   Article

   Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.

   Insert

   Cas9D10A-2A-GFP
   

   Use

   CRISPR

   Tags

   2A-GFP

   Expression

   Mammalian

   Mutation

   D10A mutation- nickase mutant

   Promoter

   CAG

   Available Since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. dRT-pMDLg/pRRE

   Plasmid

   #60488

   Purpose

   gag/pol packaging plasmid for production of lentiviral vectors with mutated reverse transcriptase (NRTLV, non-reverse transcribable lentiviral vectors)

   Depositor

   Boris Fehse

   Article

   Mock et al Sci Rep. 2014 Sep 18;4:6409. doi: 10.103

   Insert

   HIV-1 gag, HIV-1 pol with inactive reverse transcriptase
   

   Use

   Tags

   Expression

   Mutation

   changed Asp at position 249 + 250 to Val

   Promoter

   Available Since

   Oct. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pORTMAGE312B

   Plasmid

   #128969

   Purpose

   Broad-host-range KanR plasmid expressing PapRecT and EcMutL_E32K with Toluic acid induction

   Depositor

   George Church

   Article

   Wannier et al Proc Natl Acad Sci U S A. 2020 Jun 16;11

   Inserts

   PapRecT
   mutL E32K
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   E32K

   Promoter

   xylS-Pm

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAT253

    Plasmid

    #40943

    Depositor

    Angela Taddei

    Article

    Dubarry et al Genes Dev. 2011 Jul 1;25(13):1365-70.

    Insert

    GFP-LacI**
    

    Use

    Tags

    GFP(S65T, V163A) and SV40 NLS

    Expression

    Yeast

    Mutation

    LacI**/LacI2G(4 mutations) (see comment below); l…

    Promoter

    His3 promoter fragment

    Available Since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBAD-tdTEVDB_C-15A/G4C

    Plasmid

    #177205

    Purpose

    Permuted td intron with evolved initiation sequence for the expression of circular mRNA

    Depositor

    Stephen Fried

    Article

    Lee et al ACS Cent Sci. 2021 Oct 27;7(10):1736-175

    Insert

    Permuted GFP with evolved initiation sequence for circular mRNA
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    Permuted sfGFP after residue 52 & Changed Cyt…

    Promoter

    araBAD

    Available Since

    Sept. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-FLEX-SaCas9-U6-sgSlc18a2

    Plasmid

    #124849

    Purpose

    Mutagenesis of Slc18a2

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc18a2 (Slc18a2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-FLEXfrt-SaCas9-U6-sgSlc18a2

    Plasmid

    #124862

    Purpose

    Mutagenesis of Slc18a2

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc18a2 (Slc18a2 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. cpLID-micro

    Plasmid

    #168995

    Purpose

    circularly permuted LOV2 based optical dimerization system (ssrA-cpLOV2 and sspB)

    Depositor

    Yubin Zhou

    Article

    He et al Nat Chem Biol. 2021 Aug;17(8):915-923. d

    Insert

    mCh-sspB-T2A-cpLID-CAAX
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-FLEX-SaCas9-U6-sgSlc17a8

    Plasmid

    #124848

    Purpose

    Mutagenesis of Slc17a8

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Slc17a8 (Slc17a8 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCS2TAL3-DD

    Plasmid

    #37275

    Purpose

    TALEN backbone vector for use with the Golden Gate TALEN Kit in place of pTAL1-4. Shorter N- and C-terminal tal protein segments increases mutation induction; Fok I DD (improved version=pCS2TAL3-DDD)

    Depositor

    David Grunwald

    Article

    Dahlem et al PLoS Genet. 2012 Aug;8(8):e1002861. Epub

    Type

    Empty backbone

    Use

    Tags

    Flag, Fok I DD, Tal-C, and Tal-N

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 27, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAT222

    Plasmid

    #40942

    Depositor

    Angela Taddei

    Article

    Dubarry et al Genes Dev. 2011 Jul 1;25(13):1365-70.

    Insert

    GFP-LacI**
    

    Use

    Tags

    GFP(S65T, V163A) and SV40 NLS

    Expression

    Yeast

    Mutation

    LacI**/LacI2G(4 mutations) (see comment below); l…

    Promoter

    His3 promoter fragment

    Available Since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. E3020X

    Plasmid

    #21366

    Depositor

    Gregory Germino

    Article

    Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 9

    Insert

    autosomal dominant polycystic kidney disease type I (PKD1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Changed E3020 to stop codon

    Promoter

    Available Since

    Feb. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pSALECTNK-TEM1(S70A,D179G)/csTEM1

    Plasmid

    #81163

    Purpose

    pSALECT plasmid with TEM-1 beta-lactamase fused to TEM-1

    Depositor

    Timothy Whitehead

    Article

    Klesmith et al Proc Natl Acad Sci U S A. 2017 Feb 14. p

    Insert

    TEM-1 beta-lactamase
    

    Use

    Tags

    Codon swapped TEM-1 from H26 to W290 and His6x

    Expression

    Bacterial

    Mutation

    Deleted the sec transport tag. Plasmid encodes H2…

    Promoter

    lac promoter

    Available Since

    Feb. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. GluR2_adRNA(1,20,6)-ADAR2(E488Q)

    Plasmid

    #124621

    Purpose

    Expresses adRNA(1,20,6) targeting the RAB7A transcript along with the ADAR2(E488Q)

    Depositor

    Prashant Mali

    Article

    Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

    Insert

    ADAR2(E488Q)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    E488Q hyperactive mutant

    Promoter

    CMV

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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