We narrowed to 4,489 results for: TIL;

1. pGEX6P1-merlin 1-339

   Plasmid

   #168128

   Purpose

   Bacterial expression of human Nf2/merlin 1-339

   Depositor

   Tina Izard

   Article

   Primi et al Matrix Biol Plus. 2021 Jul 1;12:100074.

   Insert

   Merlin (NF2 Human)
   

   Tags

   Glutathione S-transferase (GST)

   Expression

   Bacterial

   Available Since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. NAX_CAGGS_CAS9_m4_VPR_EYFP

   Plasmid

   #167882

   Purpose

   PiggyBac compatible plasmid expressing dCas9-VPR

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-VPR
   

   Use

   CRISPR

   Mutation

   D10A, D839A, H840A, N863A

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPU-Empty-miR30-TRIM5

   Plasmid

   #132936

   Purpose

   All-in-one control rescue-TRIM5 shRNA knockdown vector

   Depositor

   Jeremy Luban

   Article

   Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

   Insert

   control shRNA
   

   Use

   Lentiviral and RNAi

   Tags

   HA

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   Oct. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-DRD1-NNES-eLOV-TEVcs-Flag-Gal4-V5

   Plasmid

   #125227

   Purpose

   Expresses SPARK DRD1 transmembrane component in mammalian cells

   Depositor

   Alice Ting

   Article

   Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

   Insert

   DRD1-NNES-eLOV-TEVcs-Flag-Gal4-V5
   

   Use

   AAV

   Tags

   Flag, V5

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. J23101

   Plasmid

   #55176

   Purpose

   Very weak constitutive promoter

   Depositor

   Thorsten Mascher

   Article

   Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

   Insert

   J23101 promoter
   

   Use

   Synthetic Biology; Bacillus biobrick box

   Promoter

   J23101

   Available Since

   July 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. KK402: pMVP/Zeo-DEST

   Plasmid

   #121859

   Purpose

   pMVP destination vector, empty expression vector backbone w/ Zeo selection cassette

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Pmvp gateway destination vector

   Expression

   Mammalian

   Available Since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTS1026-Tier1-PhCMV-3xNLS-iRFP670

   Plasmid

   #169532

   Purpose

   Tier-1 vector encoding PhCMV-driven iRFP670 that is localized to the nucleus (PhCMV-3xNLS-iRFP670-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven nucleus-localized far-red fluorescent protein iRFP670
   

   Expression

   Mammalian

   Promoter

   PhCMV

   Available Since

   June 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOCC121

   Plasmid

   #118893

   Purpose

   shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and monomeric GFP and C-terminal HIS6, cleavable with 3C

   Depositor

   Marc Bickle, PEPC MPI CBG

   Article

   Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

   Insert

   NcoI-MBP-3C-mGFP-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
   

   Tags

   HIS6, cleavable with 3C protease and MBP, cleavab…

   Expression

   Insect

   Promoter

   polH

   Available Since

   Jan. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET21-pelB-LaG-24

   Plasmid

   #172750

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-24; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-24
   

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET21-pelB-LaG-9

    Plasmid

    #172742

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-9; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-9
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. KP201: pMVP (L5-L4) eGFP-P2A

    Plasmid

    #121708

    Purpose

    pMVP L5-L4 entry plasmid, contains eGFP-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term eGFP linked by P2A to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    eGFP-P2A (open)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pL106P-LUC-ENTR1A(w451-1)

    Plasmid

    #17455

    Purpose

    Gateway entry vector, Degron N-term Luciferase

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Insert

    FKBP-Luciferase fusion (MTOR firefly)
    

    Use

    Luciferase; Entry vector

    Tags

    FKBP12DD

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. KP301: pMVP (L5-L4) mCherry-P2A

    Plasmid

    #121710

    Purpose

    pMVP L5-L4 entry plasmid, contains mCherry-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term mCherry linked by P2A to gene of interest.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    mCherry-P2A (open)
    

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOCC70

    Plasmid

    #118878

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal monomeric GFP, cleavable with TEV, and C-terminal HIS6 tag, cleavable with 3C

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-NotI-ccdB-AscI-TEV-mGFP-3C-HIS6-stop-HindIII cassette
    

    Tags

    mGFP, cleavable with TEV protease; HIS6, cleavabl…

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-PKAsub-YPet-NES

    Plasmid

    #138203

    Purpose

    Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKA activity reporter (BimAKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES.

    Depositor

    Jin Zhang

    Article

    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

    Insert

    PKAsub-YPet-NES
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET21-pelB-LaG-8

    Plasmid

    #172761

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-8; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-8
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET21-pelB-LaG-5

    Plasmid

    #172760

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-5; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-5
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. HE401: pMVP (L1-R5) shRNA

    Plasmid

    #121698

    Purpose

    pMVP L1-R5 entry plasmid, contains empty H1-driven shRNA expression cassette for 4-component MultiSite Gateway Pro assembly

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmvp gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMIII-Neo-E2Crimson

    Plasmid

    #153425

    Purpose

    Retroviral (MSCV) expression of E2-Crimson fluorescent protein

    Depositor

    Maki Nakayama

    Article

    Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

    Insert

    E2-Crimson
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Aug. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-mGold-CAF1-C-10

    Plasmid

    #158003

    Purpose

    Visualization of nucleus in mammalian cells using mGold

    Depositor

    Francois St-Pierre

    Article

    Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

    Insert

    CAF1 (Chaf1a Mouse)
    

    Tags

    mGold

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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