We narrowed to 14,519 results for: cel.2

1. pAS1-gpa-2P::his-24::Cerulean

   Plasmid

   #124337

   Purpose

   Multicolor Worms for Neuronal ID

   Depositor

   Andrew Leifer

   Insert

   gpa-2, Cerulean (gpa-2 Nematode)
   

   Use

   Tags

   Expression

   Worm

   Mutation

   Promoter

   Available since

   April 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. p5E-Runx1+23

   Plasmid

   #69602

   Purpose

   Runx1 +23 kb intronic enhancer from mouse and beta-globin minimal promoter in a 5' entry vector for gateway cloning

   Depositor

   Owen Tamplin, Leonard Zon

   Article

   Tamplin et al Cell. 2015 Jan 15;160(1-2):241-52. doi:

   Inserts

   Runx1 +23 intronic enhancer (Runx1 Mouse)
   Mouse beta-globin minimal promoter
   

   Use

   5' gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   C57/BL6 mouse Runx1 +23 intronic enhancer and C57…

   Available since

   Sept. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. SMC2 CRISPR

   Plasmid

   #140649

   Purpose

   SMC2 tagging CRISPR

   Depositor

   Masato Kanemaki

   Article

   Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

   Insert

   SMC2 (SMC2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pet28a-His6-Halo-TEV-Nrf2

   Plasmid

   #62455

   Purpose

   Halo-His6 tagged Nrf2 for E.coli expression

   Depositor

   Yimon Aye

   Article

   Parvez et al J Am Chem Soc. 2014 Dec 31.

   Insert

   Nrf2 (NFE2L2 Human)
   

   Use

   Tags

   His6-Halo-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-SNAP-CRY2-VHH(GFP)

   Plasmid

   #58370

   Purpose

   For use in light-induced inactivation of GFP fused protein through the interaction with CIB1-MP

   Depositor

   Won Do Heo

   Article

   Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

   Insert

   CRY2[1-498] (CRY2 Mustard Weed)
   

   Use

   Tags

   SNAP and vhhGFP4

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMC-cIAP1-FL

   Plasmid

   #20209

   Depositor

   Martin Holcik

   Article

   Zhao et al Cell Death Differ. 2009 Feb . 16(2):244-

   Insert

   cIAP1 3'UTR (BIRC2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 28, 2009

   Availability

   Academic Institutions and Nonprofits only

7. CCR2-DuET

   Plasmid

   #213198

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   CCR2 (CCR2 Human)
   

   Use

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHIV-EGFP.Flag-EZH2-mt2*

   Plasmid

   #220246

   Purpose

   Expresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.

   Depositor

   Chen Davidovich

   Article

   Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

   Insert

   EZH2 mt 2* (EZH2 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   PRKKKR494-499NAAIRS

   Promoter

   EF-1α

   Available since

   June 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pv6_MECP2_MBD

   Plasmid

   #179407

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MECP2_MBD

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   MECP2_MBD (Mecp2 Mouse)
   

   Use

   Tags

   AviTag and EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAGGS

   Available since

   Aug. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. CMV-10-3xFLAG-LGP2-K651E

    Plasmid

    #58685

    Purpose

    Expresses LGP2 with a mutation in the CTD (K651E) in mammalian cells

    Depositor

    Curt Horvath

    Article

    Bruns et al J Biol Chem. 2013 Jan 11;288(2):938-46.

    Insert

    LGP2-K651E (DHX58 Human)
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    mutation to CTD, K651E

    Promoter

    CMV

    Available since

    July 31, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pFB1.HMBP.PrS.EZH2-mt2

    Plasmid

    #220240

    Purpose

    Expression of an EZH2 mutant in insect cells.

    Depositor

    Chen Davidovich

    Article

    Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

    Insert

    EZH2 mt 2 (EZH2 Human)
    

    Use

    Tags

    6x His, MBP

    Expression

    Insect

    Mutation

    F32A, R34A, D36A, K39A, PRKKKR494-499NAAIRS

    Promoter

    Polyhedrin

    Available since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV6-AN-DDK_1-590 POLI

    Plasmid

    #131233

    Purpose

    Mammalian expression of DNA polymerase iota residues 1-590 with an N-terminal FLAG-tag

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    DNA polymerase iota (POLI Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains a 24 bp insertion within the Pol iota co…

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV6-AN-DDK_1-420 POLI

    Plasmid

    #131234

    Purpose

    Mammalian expression of DNA polymerase iota residues 1-420 with an N-terminal FLAG-tag

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    DNA polymerase iota (POLI Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains a 24 bp insertion within the Pol iota co…

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. GFP-HA-BACE1

    Plasmid

    #165032

    Purpose

    HA-BACE1

    Depositor

    Juan Bonifacino

    Article

    Prabhu et al Mol Biol Cell. 2012 Jun;23(12):2339-51.

    Insert

    BACE1 (BACE1 Human)
    

    Use

    Tags

    HA (embedded after propeptide cleavage site)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNW538_pAAVS1-pAP1(2)-FlpO-ABI-P2A-PYL-FlpO-CAG-U1-frt-STOP-frt-U2-GFP-P2A-luc2-ZeoR

    Plasmid

    #192939

    Purpose

    FlpO-based digitizer circuit driven by AP1 promoter

    Depositor

    Wilson Wong

    Article

    Wong et al Nat Commun. 2021 Oct 22;12(1):6150. doi:

    Inserts

    AP1(2)/minTK promoter
    FlpO-ABI
    PYL-FlpO
    CAG promoter
    frt-STOP-frt
    GFP
    luciferase
    pPGK
    Zeocin resistance
    BFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP/golgin-160/3DE

    Plasmid

    #180631

    Purpose

    Expression of caspase-resistant golgin-160

    Depositor

    Carolyn Machamer

    Article

    Mancini et al J Cell Biol. 2000 May 1;149(3):603-12. d

    Insert

    golgin-160/3DE (GOLGA3 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    D59E, D139E, D311E

    Promoter

    Available since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSBbi G418 Hu Her2

    Plasmid

    #183246

    Purpose

    Expression of human HER2 antigen in a Sleeping Beauty transposon vector

    Depositor

    Alexander McLellan

    Article

    Rad S M et al PLoS One. 2020 Jul 24;15(7):e0232915. do

    Insert

    Her2 (ERBB2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPBbleo-ErbB2

    Plasmid

    #197361

    Purpose

    Encoding human ErbB2.

    Depositor

    Michiyuki Matsuda

    Article

    Matsuda et al J Cell Sci. 2023 Jul 31:jcs.261199. doi:

    Insert

    ERBB2 (ERBB2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHR KIF17 (1-738)

    Plasmid

    #60406

    Purpose

    Expression of truncated form of human homodimeric kinesin-2 (KIF17) amino acids 1-738

    Depositor

    Ron Vale

    Article

    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

    Insert

    KIF17 (KIF17 Human)
    

    Use

    Lentiviral

    Tags

    GB1, Strep-Tag, and sfGFP

    Expression

    Mammalian

    Mutation

    Truncated version amino acids 1-738

    Promoter

    SFFV

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pIRESneo-FLAG/HA Ago2

    Plasmid

    #10821

    Depositor

    Thomas Tuschl

    Article

    Meister et al Mol Cell. 2004 Jul 23. 15(2):185-97.

    Insert

    Argonaute 2 (AGO2 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    R668H. Additionally, N terminus different from wi…

    Promoter

    Available since

    Oct. 14, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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