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pcAla12/Ala17

Plasmid

#17682

Depositor

David Shalloway

Article

Yaciuk et al Mol Cell Biol. 1989 Jun . 9(6):2453-63.

Insert

c-src S12A S17A (SRC Chicken)

Expression

Mammalian

Mutation

Ser 12 changed to Ala. Ser 17 changed to Ala.

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
pcA12/F527

Plasmid

#17683

Depositor

David Shalloway

Article

Yaciuk et al Mol Cell Biol. 1989 Jun . 9(6):2453-63.

Insert

c-src S12A Y527F (SRC Chicken)

Expression

Mammalian

Mutation

Ser 12 changed to Ala. Tyr 527 changed to Phe .

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
ZP47 Nlk1

Plasmid

#16895

Depositor

Randall Moon

Article

Thorpe et al Development. 2004 Jun;131(12):2899-909.

Insert

Nlk1 (nlk1 Zebrafish)

Expression

Bacterial

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
p218 pCMV-CREM-L

Plasmid

#8396

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

CREM-L

Use

Cre/Lox

Expression

Mammalian

Mutation

modified Cre, single LOX 511 site

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
pSKB3.MPN314

Plasmid

#11433

Depositor

Sung-Hou Kim

Article

Chen et al Proteins. 2004 Jun 1. 55(4):785-91.

Insert

cell division protein

Expression

Bacterial

Available Since

April 10, 2006

Availability

Academic Institutions and Nonprofits only
pSL7 - Brr2 del247 N1104L

Plasmid

#90257

Purpose

protein expression

Depositor

Christine Guthrie

Article

Ledoux et al J Biol Chem. 2016 Jun 3;291(23):11954-65

Insert

BRR2

Tags

TAP

Expression

Yeast

Mutation

deleted first 247 amino acids and N1104L

Availability

Academic Institutions and Nonprofits only
pWUKI Cas1 Cas2 E9Q

Plasmid

#80091

Purpose

Encodes Cas1 and Cas2 E9Q

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
CRISPR Array (bp1542-2899)

Expression

Bacterial

Mutation

E9Q

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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pWUKI Cas1 V134L Cas2

Plasmid

#80092

Purpose

Encodes Cas1 V134L and Cas2

Depositor

George Church

Article

Shipman et al Science. 2016 Jun 9. pii: aaf1175.

Inserts

Cas1 (cas1 )
Cas2 (cas2 )
CRISPR Array

Expression

Bacterial

Mutation

V134L

Promoter

T7

Availability

Academic Institutions and Nonprofits only

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SPECS library

Pooled Library

#127842

Purpose

For performing high-throughput cell state-specific synthetic promoter screening

Depositor

Timothy Lu

Article

Wu et al Nat Commun. 2019 Jun 28;10(1):2880. doi:

Species

Synthetic

Use

Lentiviral

Available Since

Aug. 22, 2020

Availability

Academic Institutions and Nonprofits only
pC004 - PFS library in Beta-lactamase gene Target Plasmid

Pooled Library

#79153

Purpose

Library of protospacer flanking sites (PFS) inserted at the 5' end of the β-lactamase gene for screening PFS preference for Cas13a/C2c2.

Depositor

Feng Zhang

Article

Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

Available Since

Oct. 26, 2017

Availability

Academic Institutions and Nonprofits only
pGL2-2xCLEAR-luciferase

Plasmid

#81120

Purpose

Reporter activity of the CLEAR network

Depositor

Sung Hee Baek

Article

Shin et al Nature. 2016 Jun 15. doi: 10.1038/nature

Insert

2xCLEAR

Use

Luciferase

Tags

luciferase

Promoter

2xCLEAR

Available Since

Dec. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pLJM1-EGFP

Plasmid

#19319

Purpose

3rd gen lentiviral vector for EGFP fusion; PGK driven puromycin

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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pFLP3

Plasmid

#64946

Purpose

Flp recombinase expression vector

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Insert

Flp recombinase

Expression

Bacterial

Promoter

lambda promoter/temperature-sensitive Lambda cI r…

Available Since

Nov. 2, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pTOX8

Plasmid

#127453

Purpose

Encodes MqsR toxin and tsPurple chromoprotein. CAM-R

Depositor

Matthew Waldor

Article

Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

Inserts

mqsR toxin
tsPurple chromoprotein

Expression

Bacterial

Mutation

Codon optimized from protein sequence in FPbase

Promoter

apFAB46 and pRha

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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CAG::PhiC31

Plasmid

#140506

Purpose

Encodes PhiC31 recombinase driven by the CAG promoter

Depositor

Mark Emerson

Article

Schick et al Sci Rep. 2019 Jun 27;9(1):9358. doi: 10.

Insert

CAG promoter

Use

Chicken expression

Expression

Mammalian

Available Since

Nov. 13, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_Lifeact_STf

Plasmid

#182011

Purpose

CMV overexpression of SNAP-tag fast in mammalian cell lines associated to actin

Depositor

Kai Johnsson

Article

Frei et al ACS Chem Biol. 2022 Jun 17;17(6):1321-13

Insert

Lifeact_STf

Expression

Mammalian

Mutation

Fast mutation on SNAP-tag E30R

Promoter

CMV

Available Since

Dec. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 Orai3

Plasmid

#16370

Depositor

Anjana Rao

Article

Gwack et al J Biol Chem. 2007 Jun 1. 282(22):16232-4

Insert

Orai3 (ORAI3 Human)

Tags

His and Myc

Expression

Mammalian

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only
pMSSM24: pCMV-SpuFz1

Plasmid

#205260

Purpose

Human expression vector for human codon optimized SpuFz1

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

SpuFz1

Expression

Mammalian

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_DL121_WTTS

Plasmid

#171954

Purpose

Selection vector for chimeric fusion of ecDHFR and asLOV2.

Depositor

Kimberly Reynolds

Article

McCormick et al Elife. 2021 Jun 16;10. pii: 68346. doi:

Inserts

Fusion of ecDHFR and asLOV2 at position 121 of DHFR
Thymidylate Synthase

Tags

8-His tag

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pGCDNsam-Hnf4a-IRES-GFP

Plasmid

#33006

Depositor

Atsushi Suzuki

Article

Sekiya et al Nature. 2011 Jun 29;475(7356):390-3. doi

Insert

hepatic nuclear factor 4 alpha (Hnf4a Mouse)

Use

Retroviral

Available Since

March 6, 2012

Availability

Academic Institutions and Nonprofits only

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