We narrowed to 24,405 results for: nes

1. pT2-shATRX

   Plasmid

   #124258

   Purpose

   Expresses shRNA targeting ATRX. Construct has inverted repeats to be used in Sleeping beauty system.

   Depositor

   Maria Castro

   Article

   Nunez et al Sci Transl Med. 2019 Feb 13;11(479). pii

   Insert

   shATRX
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR223-TSSK3

   Plasmid

   #23553

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   TSSK3 (TSSK3 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pBABE_hygro_mRFP1_NRF2(C506S, L4A)

   Plasmid

   #136580

   Purpose

   mammalian expression of mRFP1-tagged human NRF2(C506S, Leuzip mut)

   Depositor

   Kevin Janes

   Article

   Pereira et al Sci Signal. 2020 Apr 14;13(627). pii: 13

   Insert

   NRF2 (C506S, Leuzip mut) (NFE2L2 Human)
   

   Use

   Retroviral

   Tags

   N-mRFP1-FLAG

   Expression

   Mammalian

   Mutation

   The C506S mutation (C513S in the actual sequence …

   Promoter

   Available since

   July 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKLV2-U6gRNA5(hWWTR1-W2B)-PGKpuro2AmCherry-W

   Plasmid

   #163177

   Purpose

   Lentiviral gRNA plasmid targeting human WWTR1 gene, co-expression of mCherry tag

   Depositor

   Sok Ching Cheong

   Article

   Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

   Insert

   WWTR1 (WWTR1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAGGS-Omicron.BA.5-RBD

   Plasmid

   #213478

   Purpose

   Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.BA.5 variant

   Depositor

   Priyamvada Acharya

   Article

   Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

   Insert

   pCAGGS/Omicron.BA.5 RBD (S )
   

   Use

   Tags

   6 His Tag

   Expression

   Mammalian

   Mutation

   G339D,R346T,S371F,S373P,S375F,T376A,D405N,R408S,K…

   Promoter

   Available since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAGGS/EG5-RBD

   Plasmid

   #213410

   Purpose

   Mammalian cell expression of SARS-CoV-2 Spike RBD protein of OMICRON.EG5 variant

   Depositor

   Priyamvada Acharya

   Article

   Zhang et al bioRxiv [Preprint]. 2024 Mar 12:2024.02.

   Insert

   pCAGGS/EG5-RBD (S )
   

   Use

   Tags

   6 His Tag

   Expression

   Mammalian

   Mutation

   G339H,R346T,L368I,S371F,S373P,S375F,T376A,D405N,R…

   Promoter

   Available since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKLV2-U6gRNA5(hYAP1-Y2B)-PGKpuro2ABFP-W

   Plasmid

   #163178

   Purpose

   Lentiviral gRNA plasmid targeting human YAP1 gene, co-expression of BFP tag

   Depositor

   Sok Ching Cheong

   Article

   Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

   Insert

   YAP1 (YAP1 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   BFP

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLL3.7-EF-EYFP-YAP1_Y3F-PolyA

   Plasmid

   #112288

   Purpose

   Fluorescently labels human YAP1-2gamma isoform (504 aa) Y3F mutant _ tyrosines 341, 357 and 394 corresponding to tyrosines 391, 407 and 444 in this isoform

   Depositor

   Erik Sahai

   Article

   Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

   Insert

   EYFP-YAP1 (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   3 Tyrosines (341, 357 and 394 corresponding to ty…

   Promoter

   EF-1 alpha

   Available since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR223-TSSK1

   Plasmid

   #23740

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   TSSK1 (TSSK1B Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pDONR223-TSSK2

    Plasmid

    #23397

    Depositor

    David Root, William Hahn

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    TSSK2 (TSSK2 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    1MM

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

11. pLenti-CAG-NOTCH2NL-delta EGF repeats (N2NL-delta EGF)-ires-EGFP

    Plasmid

    #122955

    Purpose

    Lentiviral expression of NOTCH2NL delta EGF repeats under CAG promoter with EGFP expression

    Depositor

    Pierre Vanderhaeghen

    Article

    Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.

    Insert

    NOTCH2NL-delta EGF repeats (NOTCH2NLB Human)
    

    Use

    Lentiviral

    Tags

    6xhis tag

    Expression

    Mutation

    deletion of EGF repeats from full length NOTCH2NLB

    Promoter

    CAG promoter

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDEST26 Ago2 5A

    Plasmid

    #60651

    Purpose

    Expresses 6xHis-Ago2 (N-termi tag) mutated five serines to alanines in mammalian cells

    Depositor

    Carl Novina

    Article

    Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

    Insert

    Argonaute 2 (AGO2 Human)
    

    Use

    Tags

    6xHis (N-termi tag)

    Expression

    Mammalian

    Mutation

    mutated five serines to alanines

    Promoter

    CMV

    Available since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-nSpCas9-NoCBE

    Plasmid

    #209038

    Purpose

    Lentiviral vector expressing doxycycline-inducible human codon-optimized SpCas9(D10A)-6xNLS (Cas9 nickase) linked to ssDNA binding domain from RAD51 and uracil DNA glycosylase inhibitor (UGI)

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    nSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Tight TRE promoter

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti-nSpCas9-APOBEC1

    Plasmid

    #209039

    Purpose

    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where APOBEC1 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    APOBEC1-nSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Tight TRE promoter

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-nSpCas9-BEACON2

    Plasmid

    #209041

    Purpose

    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where BEACON2 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    BEACON2-nSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Tight TRE promoter

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLenti-nSpCas9-evoCDA1

    Plasmid

    #209042

    Purpose

    Lentiviral vector expressing doxycycline-inducible human codon-optimized cytosine base editor where evoCDA1 is fused to the N-terminus of SpCas9(D10A)-sDNA Rad51-2xUGI-6xNLS

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    evoCDA1-nSpCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    Tight TRE promoter

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pKLV2-U6gRNA5(hCHAT)-PGKpuro2ABFP-W

    Plasmid

    #163147

    Purpose

    Lentiviral gRNA plasmid targeting human CHAT gene, co-expression of BFP tag

    Depositor

    Sok Ching Cheong

    Article

    Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

    Insert

    CHAT (CHAT Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    BFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pKLV2-U6gRNA5(hWWTR1-W2B)-PGKpuro2ABFP-W

    Plasmid

    #163175

    Purpose

    Lentiviral gRNA plasmid targeting human WWTR1 gene, co-expression of BFP tag

    Depositor

    Sok Ching Cheong

    Article

    Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

    Insert

    WWTR1 (WWTR1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    BFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKLV2-U6gRNA5(hWWTR1-W1K)-PGKpuro2ABFP-W

    Plasmid

    #163174

    Purpose

    Lentiviral gRNA plasmid targeting human WWTR1 gene, co-expression of BFP tag

    Depositor

    Sok Ching Cheong

    Article

    Chai et al Elife. 2020 Sep 29;9. pii: 57761. doi: 1

    Insert

    WWTR1 (WWTR1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    BFP

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Sept. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Neurog2 x2)

    Plasmid

    #171100

    Purpose

    CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Neurog2.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9 (Neurog2 S. pyogenes, Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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