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Plasmid#213303PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertGRM5 (GRM5 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMeCP2G pc1121
Plasmid#167566PurposeThe complete rat MeCp2 ORF is fused in frame of the NH2-terminus of the enhanced GFP (isolated from pEGFP-N1 vector; CLONTECH Laboratories, Inc.)DepositorInsertsUseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET19b_4CL:P3S
Plasmid#139796Purposeexpression of biosynthetic enzyme 4CL in fusion with CC segment P3SDepositorInsert4CL:P3S
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV-OMM-GCaMP6f
Plasmid#127874PurposeFluorescent reporter for mitochondrial outer membrane Ca2+DepositorInsertOuter membrane localized GCaMP6f
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET19b_STS:P4S
Plasmid#139797Purposeexpression of biosynthetic enzyme STS in fusion with CC segment P4SDepositorInsertSTS:P4S
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET19b_4CL:STS (DF)
Plasmid#139791Purposedirect fusion of biosynthetic enzymes 4CL and STSDepositorInsertdirect fusion of 4CL and STS
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMV-Mito4x-GCaMP6f
Plasmid#127870PurposeFluorescent reporter for mitochondrial matrix Ca2+DepositorInsert4 repeats of COX8 signal peptide plus GCaMP6f
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET19b_4CL + STS
Plasmid#139790Purposeexpression of biosynthetic enzymes 4CL and STSDepositorInserts4CL
STS
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
YP5
Plasmid#199946PurposeYeast/E coli shuttle vector expressing GFP from synthetic yeast promoter 5DepositorInsertGFP
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMST1211_BB2_E_AB_pcoxA:pyrGtrunc_AMA1_2.8
Plasmid#90285PurposeBB2 with split pyrG marker for homologous integration with linker AB (negative control)DepositorInsertpyrGtrunc
UseTagsExpressionBacterialMutationtruncated version of pyrG gene (sequence is from …PromoterAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMST1212_BB2_E_BC_pcoxA:pyrGtrunc_AMA1_2.8
Plasmid#90286PurposeBB2 with split pyrG marker for homologous integration with linker BC (for 1 expression cassette)DepositorInsertpyrGtrunc
UseTagsExpressionBacterialMutationtruncated version of pyrG gene (sequence is from …PromoterAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMST1213_BB2_E_CD_pcoxA:pyrGtrunc_AMA1_2.8
Plasmid#90287PurposeBB2 with split pyrG marker for homologous integration with linker CD (for 2 expression cassettes)DepositorInsertpyrGtrunc
UseTagsExpressionBacterialMutationtruncated version of pyrG gene (sequence is from …PromoterAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMST1214_BB2_E_DE_pcoxA:pyrGtrunc_AMA1_2.8
Plasmid#90288PurposeBB2 with split pyrG marker for homologous integration with linker DE (for 3 expression cassettes)DepositorInsertpyrGtrunc
UseTagsExpressionBacterialMutationtruncated version of pyrG gene (sequence is from …PromoterAvailable sinceJuly 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pH-eCFP
Plasmid#176555PurposeExpression of eCFP for auxotrophic selection in the absence of histidineDepositorInserteCFP
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pL-eCFP
Plasmid#176556PurposeExpression of eCFP for auxotrophic selection in the absence of leucineDepositorInserteCFP
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU-eCFP
Plasmid#176557PurposeExpression of eCFP for auxotrophic selection in the absence of uracilDepositorInserteCFP
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU-mRuby2
Plasmid#176549PurposeExpression of mRuby2 for auxotrophic selection in the absence of uracilDepositorInsertyomRuby2
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pH-mRuby2
Plasmid#176547PurposeExpression of mRuby2 for auxotrophic selection in the absence of histidineDepositorInsertyomRuby2
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceApril 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pM-mRuby2
Plasmid#176550PurposeExpression of mRuby2 for auxotrophic selection in the absence of methionineDepositorInsertyomRuby2
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only