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Showing: 481 - 520 of 23042 results

  1. GANLS-DEVD-BNES

    Plasmid
    #50835
    Purpose
    Expresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 2 (BNLS).
    Depositor
    Robert Campbell
    Article
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Insert
    ddGFP A and ddRFP B
    Tags
    NES sequence LALKLAGLDIGS and triplicated NLS seq…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    50835-plasmid-map-sequence-id-76278

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  9. single polypeptide FPX biosensor for caspase-3

    Plasmid
    #60883
    Purpose
    FPX biosensor, a new class of genetically encoded fluorescent biosensor based on reversible exchange of the heterodimeric partners of green and red dimerization dependent fluorescent proteins (ddFPs).
    Depositor
    Robert Campbell
    Article
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Insert
    single polypeptide FPX biosensor for caspase-3
    Tags
    HindIII site and NES sequence LALKLAGLDIGS
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    60883-plasmid-map-sequence-id-150659

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  10. pX037

    Plasmid
    #167156
    Purpose
    MoClo-compatible Level2 vector for autonomous bioluminescence in plants encoding kanamycin resistance cassette, nnHispS, nnH3H, nnLuz and nnCPH. Does not contain NpgA.
    Depositor
    Karen Sarkisyan
    Article
    Mitiouchkina et al Nat Biotechnol. 2020 Apr 27. pii: 10.103
    Insert
    caffeic acid cycle genes
    Expression
    Plant
    Promoter
    pNOS, p35s
    Available Since
    June 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    167156-plasmid-map-sequence-id-335288

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  11. RANLS-IETD-BNES

    Plasmid
    #60972
    Purpose
    Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-8 biosensor, used together with plasmid 2 (BNLS).
    Depositor
    Robert Campbell
    Article
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Insert
    ddRFP A and ddRFP B
    Tags
    NES sequence LALKLAGLDIGS and triplicated NLS seq…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  12. pAAV-hSyn-LMO3 (sbGLuc-VChR1-EYFP)

    Plasmid
    #114099
    Purpose
    fusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics
    Depositor
    Ute Hochgeschwender
    Article
    Berglund et al Proc Natl Acad Sci U S A. 2016 Jan 19;11
    Insert
    Gaussia luciferase, slow burn; Volvox Channelrhodopsin 1; EYFP
    Use
    AAV
    Expression
    Mammalian
    Promoter
    hSyn
    Available Since
    Aug. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    114099-plasmid-map-sequence-id-252568

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  13. pNK3071

    Plasmid
    #219755
    Purpose
    MoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, mcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.
    Depositor
    Alexander Mishin
    Article
    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi
    Insert
    mcitHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH
    Expression
    Plant
    Available Since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    219755-plasmid-map-sequence-id-450717

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  14. pNK497

    Plasmid
    #219754
    Purpose
    MoClo-compatible Level P vector for improved autonomous bioluminescence in plants encoding kanamycin resistance cassette, nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH.
    Depositor
    Alexander Mishin
    Article
    Shakhova et al Nat Methods. 2024 Mar;21(3):406-410. doi
    Insert
    nnHispS, NpgA, nnH3H_v2, nnLuz_v4 and nnCPH
    Expression
    Plant
    Available Since
    Jan. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    219754-plasmid-map-sequence-id-458646

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  15. RANLS-LEHD-BNES

    Plasmid
    #60973
    Purpose
    Expresses a tandem ddFP heterodimer in mammalian cells, plasmid 1 for a translocation-based red fluorescent caspase-9 biosensor, used together with plasmid 2 (BNLS).
    Depositor
    Robert Campbell
    Article
    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n
    Insert
    ddRFP A and ddRFP B
    Tags
    Caspase9 substrate sequence (LEHD) is after NLS s…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 2, 2015
    Availability
    Academic Institutions and Nonprofits only

  20. cmamxA2-GFP

    Plasmid
    #107197
    Purpose
    Expresses human annexin A2-GFP fusion protein for live cell imaging, all type II Ca2+ binding sites deleted
    Depositor
    Volker Gerke, Ursula Rescher
    Article
    Rescher et al J Cell Sci. 2000 Nov;113 ( Pt 22):3931-8
    Insert
    cmannexin A2 (ANXA2 Human)
    Tags
    Green Fluorescent Protein
    Expression
    Mammalian
    Mutation
    all type II Ca2+ binding sites deleted ( D161A, E…
    Promoter
    CMV
    Available Since
    June 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    107197-plasmid-map-sequence-id-209669

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  22. pEVBC1_moxBFP

    Plasmid
    #248345
    Purpose
    Blue fluorescent protein control for Fluorescent Protein Library
    Depositor
    Calin Plesa
    Insert
    moxBFP
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    P_LacUV5
    Available Since
    Nov. 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    248345-plasmid-map-sequence-id-493220

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  23. pLY1244-T4gp44/62

    Plasmid
    #228205
    Purpose
    Expression in BL21(DE3) of WT T4 clamp loader consisting of gp44 and gp62
    Depositor
    Stephen Benkovic
    Insert
    44/62
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    228205-plasmid-map-sequence-id-472133

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  24. pET26b-T4gp45

    Plasmid
    #228204
    Purpose
    Expression in BL21(DE3) of WT T4 clamp
    Depositor
    Stephen Benkovic
    Insert
    45 (45 Escherichia phage T4)
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    228204-plasmid-map-sequence-id-453907

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  25. pMSP1E3D1_mCherry

    Plasmid
    #248929
    Purpose
    Bacterial expression of fluorescent membrane scaffold protein MSP1E3D1 fused to mCherry for nanodisc formation
    Depositor
    Michael Marty
    Insert
    MSP1E3D1_mCherry
    Tags
    His
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Dec. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    248929-plasmid-map-sequence-id-494405

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  26. pMSP1E3D1_mBlueberry2

    Plasmid
    #248930
    Purpose
    Bacterial expression of fluorescent membrane scaffold protein MSP1E3D1 fused to mBlueberry 2 for nanodisc formation
    Depositor
    Michael Marty
    Insert
    MSP1E3D1_mBlueberry2
    Tags
    His
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Dec. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    248930-plasmid-map-sequence-id-494407

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  27. pMSP1D1_sfGFP

    Plasmid
    #248920
    Purpose
    Bacterial expression of fluorescent membrane scaffold protein MSP1D1 fused to sfGFP for nanodisc formation
    Depositor
    Michael Marty
    Insert
    MSP1D1_sfGFP
    Tags
    His
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Dec. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    248920-plasmid-map-sequence-id-494374

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  28. pMSP1D1_mCherry

    Plasmid
    #248922
    Purpose
    Bacterial expression of fluorescent membrane scaffold protein MSP1D1 fused to mCherry for nanodisc formation
    Depositor
    Michael Marty
    Insert
    MSP1D1_mCherry
    Tags
    His
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Dec. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    248922-plasmid-map-sequence-id-494373

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  29. pMSP1D1_mBlueberry2

    Plasmid
    #248925
    Purpose
    Bacterial expression of fluorescent membrane scaffold protein MSP1D1 fused to mBlueberry2 for nanodisc formation
    Depositor
    Michael Marty
    Insert
    MSP1D1_mBlueberry2
    Tags
    His
    Expression
    Bacterial
    Promoter
    T7 Promoter
    Available Since
    Dec. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    248925-plasmid-map-sequence-id-494372

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  30. pTYB3-T4gp43(WT)

    Plasmid
    #228203
    Purpose
    Expression in BL21(DE3) of WT T4 DNA polymerase
    Depositor
    Stephen Benkovic
    Insert
    43
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    228203-plasmid-map-sequence-id-472938

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  31. pET28b-T4Dda

    Plasmid
    #228215
    Purpose
    Expression in BL21(DE3) of T4 Dda-like helicase
    Depositor
    Stephen Benkovic
    Insert
    dda (dda Escherichia phage T4)
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    228215-plasmid-map-sequence-id-456000

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  32. pTYB1-T4denV

    Plasmid
    #228216
    Purpose
    Expression in BL21(DE3) of T4 endonuclease V N-glycosylase UV repair enzyme
    Depositor
    Stephen Benkovic
    Insert
    denV
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    228216-plasmid-map-sequence-id-455996

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  33. pET-Impact-T4gp41

    Plasmid
    #228207
    Purpose
    Expression in BL21(DE3) of T4 helicase
    Depositor
    Stephen Benkovic
    Insert
    41 (41 Escherichia phage T4)
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    228207-plasmid-map-sequence-id-453945

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  34. pET-Impact-T4gp59

    Plasmid
    #228206
    Purpose
    Expression in BL21(DE3) of WT T4 helicase loader
    Depositor
    Stephen Benkovic
    Insert
    59 (59 Escherichia phage T4)
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    228206-plasmid-map-sequence-id-453944

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  35. pTYB1-T4uvsX

    Plasmid
    #228213
    Purpose
    Expression in BL21(DE3) of T4 recombinase
    Depositor
    Stephen Benkovic
    Insert
    uvsX (uvsX Escherichia phage T4)
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    228213-plasmid-map-sequence-id-455994

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  36. ecALDH

    Plasmid
    #67921
    Purpose
    Expresses aldehyde dehydrogenase from Escherichia coli, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Insert
    Escherichia coli aldehyde dehydrogenase (puuC Escherichia coli)
    Tags
    6x-His
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Sept. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    67921-plasmid-map-sequence-id-197668

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  37. pcDNA3-ps-CFP

    Plasmid
    #13034
    Purpose
    Expresses photo-switchable CFP in mammalian cells. Can undergo irreversible photoconversion from cyan to green fluorescence
    Depositor
    Doug Golenbock
    Insert
    photo-switchable cyan fluorescent protein
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 2, 2013
    Availability
    Academic Institutions and Nonprofits only
    13034-plasmid-map-sequence-id-7430

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  38. pTBY1-T4uvsY

    Plasmid
    #228214
    Purpose
    Expression in BL21(DE3) of T4 UvsY-like recombination mediator
    Depositor
    Stephen Benkovic
    Insert
    uvsY (uvsY Escherichia phage T4)
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Dec. 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    228214-plasmid-map-sequence-id-455995

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Showing: 481 - 520 of 23042 results