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mNG-PH-CYTH3-2G
Plasmid#233261PurposeExpression of mNeonGreen-tagged PIP3 reporterDepositorInsertPIP3 reporter
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTrcHis_VectorA_short_speGpromoter_SAT1 (human)
Plasmid#220477Purposeheterologous expression of SSAT1 under endogenous speG E. coli promoterDepositorInsertSSAT1 (SAT1 Human)
UseTagsExpressionBacterialMutationnonePromoterAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
JAB3479
Plasmid#190417PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_6XAO-2XPO::TetR-NLS-19St_proT::GFPint-ADHt)DepositorInsertproUBQ10::PIP2A-mCherry-NOSt_6XAO-2XPO::TetR-NLS-19St_proT::GFPint-ADHt
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB3278
Plasmid#190402PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-ERF2(AD)-19St_1XAmtRO(M2)::GFPint-NLS-ADHt)DepositorInsertproUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-ERF2(AD)-19St_1XAmtRO(M2)::GFPint-NLS-ADHt
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB3616
Plasmid#190426PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_6XAmtRO-2XPhlFO::TetR-NLS-19St_proT::GFPint-ADHt)DepositorInsertproUBQ10::PIP2A-mCherry-NOSt_6XAmtRO-2XPhlFO::TetR-NLS-19St_proT::GFPint-ADHt
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB2601
Plasmid#190388PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_3XAmtRO::GFPint-NLS-ADHt)DepositorInsertproUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_3XAmtRO::GFPint-NLS-ADHt
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB2600
Plasmid#190387PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_2XAmtRO::GFPint-NLS-ADHt)DepositorInsertproUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_2XAmtRO::GFPint-NLS-ADHt
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
JAB2599
Plasmid#190386PurposePlasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_1XAmtRO::GFPint-NLS-ADHt)DepositorInsertproUBQ10::PIP2A-mCherry-NOSt_proG1090::AmtR-VP16-19St_1XAmtRO::GFPint-NLS-ADHt
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-43
Plasmid#172759PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-43; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-43
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-35
Plasmid#172755PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-35; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-35
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-19
Plasmid#172748PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-19; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-19
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-3
Plasmid#172740PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-3; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-3
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-12
Plasmid#172744PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-12; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-12
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-14
Plasmid#172745PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-14; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-14
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
6xHis-SNAP-PI(3)P Probe
Plasmid#211508PurposeFor expression of recombinant biosensor for detection of Phosphatidylinositol-3-Phosphate (PI(3)P) from bacteriaDepositorInsertFYVE-Hrs x 2 (PIKFYVE Human)
UseTags6xhis-SNAPExpressionBacterialMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLentiCRISPRv1 sgTollip
Plasmid#196546PurposeLentiviral CRISPR-Cas9 plasmid containing gRNA targeting exon 1 of human Tollip. Used for generation of Tollip protein knockouts in human cell lines.DepositorInsertToll-Interacting Protein (TOLLIP Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGG-NFIX2
Plasmid#112699PurposeExpression of NFIX2 protein and GFPDepositorInsertNuclear Factor I X2 (Nfix Mouse)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
GFP-Tollip M240A,F241A
Plasmid#178051PurposeGFP fused to human Tollip cDNA carrying mutations in the CUE domain. Localisation and expression in mammalian cells.DepositorInsertToll-Interacting Protein (TOLLIP Human)
UseTagsGFPExpressionMammalianMutationChanged Methionine 240 to Alanine and changed Phe…PromoterCMVAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pIRESneo2 mycBioID-Tollip
Plasmid#178054Purposemyc-BioID fused to human Tollip cloned into the pIRESneo2 plasmid. For generation of stable expressing mammalian cells used for proximity dependent biotin labelling.DepositorInsertToll-Interacting Protein (TOLLIP Human)
UseTagsmycBioIDExpressionMammalianMutationPromoterCMVAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pGEMHE-RnCENP-O-GFP
Plasmid#205179PurposeExpresses rat CENP-O C-term tagged with GFP under T7 promoter - designed for in vitro transcriptionDepositorInsertCENP-O (Cenpo Rat)
UseIn vitro transcriptionTags5 glycines linker followed by GFPExpressionMammalianMutationWild type rat CENP-OPromoterT7Available sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pNB_EA_3EGFP
Plasmid#185967PurposeExpresses EGFP from PR promoter in E.coli Dh5alpha cellDepositorInsertPr-Egfp cassette in forward direction in Network Brick
UseSynthetic BiologyTagsExpressionMutationPromoterPRAvailable sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XFL-wtSrc
Plasmid#140295PurposeExpresses Flag-tagged wild-type human Src in mammalian cells for structure-function studiesDepositorInsertSrc wildtype (SRC Human)
UseTags2X FlagExpressionMammalianMutationPromoterCMVAvailable sinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XFL-Src-Y530F
Plasmid#140319PurposeExpresses Flag-tagged human Src-Y530F in mammalian cells for structure-function studiesDepositorInsertSrc mutant (SRC Human)
UseTags2X FlagExpressionMammalianMutationY530FPromoterCMVAvailable sinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XHA-Src-Y530F
Plasmid#140318PurposeExpresses HA-tagged human Src-Y530F in mammalian cells for structure-function studiesDepositorInsertSrc mutant (SRC Human)
UseTags2X HAExpressionMammalianMutationY530FPromoterCMVAvailable sinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XHA-wtSrc
Plasmid#140294PurposeExpresses HA-tagged wild-type human Src in mammalian cells for structure-function studiesDepositorInsertSrc wildtype (SRC Human)
UseTags2X HAExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XFL-Src-S4-KD
Plasmid#140297PurposeExpresses Flag-tagged human Src-S4-KD in mammalian cells for structure-function studiesDepositorInsertSrc deletion mutant (SRC Human)
UseTags2X FlagExpressionMammalianMutationcontains only the SH4 and KD regionsPromoterCMVAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XHA-Src-K298M
Plasmid#140314PurposeExpresses HA-tagged human Src-K298M in mammalian cells for structure-function studiesDepositorInsertSrc mutant (SRC Human)
UseTags2X HAExpressionMammalianMutationK298MPromoterCMVAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XFL-Src-K298M
Plasmid#140315PurposeExpresses Flag-tagged human Src-K298M in mammalian cells for structure-function studiesDepositorInsertSrc mutant (SRC Human)
UseTags2X FlagExpressionMammalianMutationK298MPromoterCMVAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XHA-Src-Y419F
Plasmid#140316PurposeExpresses HA-tagged human Src-Y419F in mammalian cells for structure-function studiesDepositorInsertSrc mutant (SRC Human)
UseTags2X HAExpressionMammalianMutationY419FPromoterCMVAvailable sinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
scADGFP
Plasmid#114434PurposeEpilepsy promoter enhanced with Arc elements driving GFP with SV40E polyADepositorInsertGreen fluorescent protein
UseAAVTagsER export sequence FCYENEVExpressionMammalianMutationPromoterNovel synthetic activity-dependent promoterAvailable sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XFL-Src-KD
Plasmid#140313PurposeExpresses Flag-tagged human Src-KD in mammalian cells for structure-function studiesDepositorInsertSrc mutant (SRC Human)
UseTags2X FlagExpressionMammalianMutationContains only the KD regionPromoterCMVAvailable sinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XFL-Src-Y419F
Plasmid#140317PurposeExpresses Flag-tagged human Src-Y419F in mammalian cells for structure-function studiesDepositorInsertSrc mutant (SRC Human)
UseTags2X FlagExpressionMammalianMutationY419FPromoterCMVAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XFL-Src-Y530EEI
Plasmid#140323PurposeExpresses Flag-tagged human Src-Y530EEI in mammalian cells for structure-function studiesDepositorInsertSrc mutant (SRC Human)
UseTags2X FlagExpressionMammalianMutationY530EEIPromoterCMVAvailable sinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS2+ cMyc Dam_f_GFPoNLS
Plasmid#85820PurposeiDamID plasmid. To express transiently the c-Myc-tagged , L122A-mutant E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.DepositorInsertcMyc Dam_f_GFPoNLS
UseTagscMyc and oNLS (optimized nuclear localization sig…ExpressionBacterial, Insect, Mammalia…MutationL122A.PromoterAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XHA-Src-KD
Plasmid#140312PurposeExpresses HA-tagged human Src-KD in mammalian cells for structure-function studiesDepositorInsertSrc mutant (SRC Human)
UseTags2X HAExpressionMammalianMutationContains only the KD regionPromoterCMVAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XHA-Src-S4-UD
Plasmid#140304PurposeExpresses HA-tagged human Src-S4-UD in mammalian cells for structure-function studiesDepositorInsertSrc deletion mutant (SRC Human)
UseTags2X HAExpressionMammalianMutationcontains only the SH4 and UD regionsPromoterCMVAvailable sinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XHA-Src-S4-KD
Plasmid#140296PurposeExpresses HA-tagged human Src-S4-KD in mammalian cells for structure-function studiesDepositorInsertSrc deletion mutant (SRC Human)
UseTags2X HAExpressionMammalianMutationcontains only the SH4 and KD regionsPromoterCMVAvailable sinceApril 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-2XHA-Src-S4-UD-KD
Plasmid#140300PurposeExpresses HA-tagged human Src-S4-UD-KD in mammalian cells for structure-function studiesDepositorInsertSrc deletion mutant (SRC Human)
UseTags2X HAExpressionMammalianMutationlacks the SH3 and SH2 domainsPromoterCMVAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only
