We narrowed to 1,671 results for: Anti-CRISPR

1. CMV-NLS-AcrIIA4

   Plasmid

   #113037

   Purpose

   Expression of SV40NLS-AcrIIA4 in mammalian cells (CMV promoter driven)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   SV40 NLS-AcrIIA4
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPlacPbuCas13b-gRNA-T-MS2

   Plasmid

   #184839

   Purpose

   Expression of a single-spacer CRISPR array with spacer #1 targeting the MS2 phage genome and expression of PbuCas13b in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

   Insert

   single-spacer CRISPR array with spacer #1 targeting the MS2 phage genome, PbuCas13b nuclease
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   J23119 and lac promoter

   Available Since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPlacPbuCas13b-gRNA-T-MS2

   Plasmid

   #184839

   Purpose

   Expression of a single-spacer CRISPR array with spacer #1 targeting the MS2 phage genome and expression of PbuCas13b in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

   Insert

   single-spacer CRISPR array with spacer #1 targeting the MS2 phage genome, PbuCas13b nuclease
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   J23119 and lac promoter

   Available Since

   Oct. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEJS482-pCDest2-AcrIIC3Nme-mTagBFP2-IRES

   Plasmid

   #85750

   Purpose

   Expresses Type II-C anti-CRISPR protein, AcrIIC3Nme from Neisseria meningitidis, and mTagBFP2

   Depositor

   Erik Sontheimer

   Article

   Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

   Inserts

   Type II-C anti-CRISPR AcrIIC3Nme
   mTagBFP2
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEJS482-pCDest2-AcrIIC3Nme-mTagBFP2-IRES

   Plasmid

   #85750

   Purpose

   Expresses Type II-C anti-CRISPR protein, AcrIIC3Nme from Neisseria meningitidis, and mTagBFP2

   Depositor

   Erik Sontheimer

   Article

   Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

   Inserts

   Type II-C anti-CRISPR AcrIIC3Nme
   mTagBFP2
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEJS481-pCDest2-AcrE2-mTagBFP2-IRES

   Plasmid

   #85749

   Purpose

   Expresses anti-CRISPR protein from Type I-E and mTagBFP2

   Depositor

   Erik Sontheimer

   Article

   Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

   Inserts

   Type I-E anti-CRISPR
   mTagBFP2
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEJS481-pCDest2-AcrE2-mTagBFP2-IRES

   Plasmid

   #85749

   Purpose

   Expresses anti-CRISPR protein from Type I-E and mTagBFP2

   Depositor

   Erik Sontheimer

   Article

   Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

   Inserts

   Type I-E anti-CRISPR
   mTagBFP2
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pPbuCas13b-gRNA-1

   Plasmid

   #184567

   Purpose

   Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

   Insert

   Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPbuCas13b-gRNA-1

   Plasmid

   #184567

   Purpose

   Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.

   Depositor

   Chase Beisel

   Article

   Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

   Insert

   Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   J23119

   Available Since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CMV-CASANOVA

    Plasmid

    #113035

    Purpose

    CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    CASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. CMV-CASANOVA

    Plasmid

    #113035

    Purpose

    CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    CASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. p1195-pssAAV.U1a.hNme2Cas9

    Plasmid

    #129534

    Purpose

    AAV vector expressing Nme2Cas9

    Depositor

    Erik Sontheimer

    Article

    Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

    Insert

    human codon-optimized Nme2Cas9
    

    Use

    AAV

    Tags

    2xNLS and NLS-3xHA-NLS

    Promoter

    U1a

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. p1195-pssAAV.U1a.hNme2Cas9

    Plasmid

    #129534

    Purpose

    AAV vector expressing Nme2Cas9

    Depositor

    Erik Sontheimer

    Article

    Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

    Insert

    human codon-optimized Nme2Cas9
    

    Use

    AAV

    Tags

    2xNLS and NLS-3xHA-NLS

    Promoter

    U1a

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPbuCas13b

    Plasmid

    #184565

    Purpose

    Inducible expression of PbuCas13b nuclease in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    Cas13b nuclease from Prevotella buccae
    

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPbuCas13b

    Plasmid

    #184565

    Purpose

    Inducible expression of PbuCas13b nuclease in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    Cas13b nuclease from Prevotella buccae
    

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Nov. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. IL1RN gRNA1

    Plasmid

    #113130

    Purpose

    Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    IL1RN guideRNA 1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Expression

    Mammalian

    Promoter

    U6 for gRNA expression, RSV for GFP expression

    Available Since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. IL1RN gRNA1

    Plasmid

    #113130

    Purpose

    Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    IL1RN guideRNA 1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Expression

    Mammalian

    Promoter

    U6 for gRNA expression, RSV for GFP expression

    Available Since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV N-SpyCas9-Intein, U6-gRNA scaffold (F+E)

    Plasmid

    #120293

    Purpose

    AAV Vector for expression of N-terminal SpyCas9 fragment with Intein and a U6-driven F+E gRNA scaffold

    Depositor

    Dominik Niopek

    Article

    Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

    Insert

    N-terminal fragment of SpyCas9
    

    Use

    AAV and CRISPR

    Tags

    SV40-NLS and split-intein

    Expression

    Mammalian

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AAV N-SpyCas9-Intein, U6-gRNA scaffold (F+E)

    Plasmid

    #120293

    Purpose

    AAV Vector for expression of N-terminal SpyCas9 fragment with Intein and a U6-driven F+E gRNA scaffold

    Depositor

    Dominik Niopek

    Article

    Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

    Insert

    N-terminal fragment of SpyCas9
    

    Use

    AAV and CRISPR

    Tags

    SV40-NLS and split-intein

    Expression

    Mammalian

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-RSV-GFP-U6-Rosa26 gRNA (SpyCas9 scaffold)

    Plasmid

    #120296

    Purpose

    AAV vector; encodes GFP as well as a U6-driven Rosa26-targeting gRNA (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

    Insert

    Rosa26 gRNA (SpCas9 scaffold)
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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