We narrowed to 248 results for: ESP3;

1. pEND-351_Cacnes_dCas9_CRISPRi

   Plasmid

   #225617

   Purpose

   Cutibacterium acnes replicative plasmid with dCas9 for CRISPRi. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a medium-copy pBR322 E. coli ori (no ROP)

   Depositor

   Marc Güell

   Article

   Guillermo et al Cell Systems, 2025

   Insert

   dCas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Codon optimized for Cutibacterium acnes

   Promoter

   Available since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. LCV2_BFP2

   Plasmid

   #155097

   Purpose

   Lentiviral backbone for cloning and expression of U6 driven gRNAs with Esp3I/BsmBI cloning sites, puromycin selection and BFP2 expression

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pINSPECT_SigP:NLuc

   Plasmid

   #197263

   Purpose

   Cloning Backbone for INSPECT expression. Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette. Clone homology arms via Esp3I.

   Depositor

   Gil Westmeyer

   Article

   Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676

   Type

   Empty backbone

   Use

   CRISPR, Luciferase, Synthetic Biology, and Unspec…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC19-U6-[BsmBI_pegRNA_entry]-tevopreQ1-term (LM1138)

   Plasmid

   #223137

   Purpose

   pU6 entry plamid for cloning prime editor tevopreQ1 epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   etevopreQ1 entry plasmid backbone for epegRNA expression via human U6 promoter
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC19-U6-BsmBI_epegRNA_entry-mpknot_(LM1140)

   Plasmid

   #228253

   Purpose

   pU6 entry plamid for cloning prime editor mpknot epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   mpknot entry plasmid backbone for epegRNA expression via human U6 promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   n/a

   Promoter

   U6

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR223-CMV-Cre-U6-sgX-U6-sgRb1-U6-sgTrp53-U6-sgRbl2

   Plasmid

   #209060

   Purpose

   Entry cloning vector to insert an sgRNA of interest (using Esp3i digestion) into a vector that already contains sgRNAs against mouse Rb1, Trp53, and Rbl2 and CMV Cre recombinase.

   Depositor

   Matthew Oser

   Article

   Duplaquet et al Nat Cell Biol. 2023 Sep;25(9):1346-1358.

   Insert

   sgRNAs targeting Rb1, Trp53, Rbl2 and Cre recombinase
   

   Use

   Gateway vector to be used for lr reaction

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Nov. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. EC71174

   Plasmid

   #154067

   Purpose

   Level 2 Golden Gate vector, pL2M-HvHSP17-CREU5-UBQ-loxmCHERRYER-eGFPER

   Depositor

   Cristobal Uauy

   Article

   Harrington et al Plant Methods. 2020 Oct 14;16:137. doi:

   Inserts

   HS Cre recombinase
   Ubiquitin promoter driving mCherry with the 35S terminator, flanked by lox sites, followed by eGFP with the Actin terminator
   HYGROMYCIN Resistance Cassette
   

   Use

   Cre/Lox and Synthetic Biology; Golden gate

   Tags

   Expression

   Bacterial and Plant

   Mutation

   The Arabidopsis thaliana U5 small nuclear ribonuc…

   Promoter

   35S promoter, Hordeum vulgare HSP 17 promoter use…

   Available since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. Joint Modular Cloning (JMC) Toolkit

   Plasmid Kit

   #1000000233

   Purpose

   The Joint Modular Cloning (JMC) Toolkit includes a modular cloning chassis and set of functional parts for assembling plant transformation vectors.

   Depositor

   Daniel Voytas

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only