We narrowed to 40,599 results for: LIS

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eTd-CBEa

Plasmid

#196603

Purpose

Mammalian expression of eTd-CBEa

Depositor

Dali Li

Article

Chen et al Nat Biotechnol. 2022 Nov 10. doi: 10.103

Insert

bpNLS-TadA-8e (N46L/P29A)-nSpCas9-bpNLS-P2A-UGI-bpNLS

Tags

6*His

Expression

Mammalian

Available Since

March 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_g5-HT2h

Plasmid

#208721

Purpose

Expresses the green 5-HT sensor GRAB_g5-HT2h in neurons

Depositor

Yulong Li

Article

Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

Insert

Green fluorescent 5-HT sensor GRAB_g5-HT2h

Use

AAV

Promoter

hSyn

Available Since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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AID-BE4max-P2A-GFP

Plasmid

#157948

Purpose

Mammalian expression of AID-BE4max-P2A-GFP

Depositor

Dali Li

Article

Zhang et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

AID-BE4max

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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WP_010880027.1

Plasmid

#194729

Purpose

Lumazine synthase of the hyperthermophile Aquifex aeolicus bacterium

Depositor

Fang Li

Article

Geng et al PLoS Pathog. 2021 Sep 7;17(9):e1009897.

Insert

nanoparticle-proteinA and His tag

Tags

His tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pETSG

Plasmid

#159418

Purpose

Expression of proteins with a C-terminal thermostable GFP (TGP) tag in Escherichia coli

Depositor

Dianfan Li

Article

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

thermostable GFP (TGP)

Tags

polyhistidine (poly-His)

Expression

Bacterial

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-GfaABC1D-GRAB_eCB2.0

Plasmid

#164609

Purpose

Express the endocannabinoid sensor GRAB_eCB2.0 in astrocytes

Depositor

Yulong Li

Article

Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

Insert

Endocannabinoid sensor GRAB_eCB2.0

Use

AAV

Mutation

S383T

Promoter

GfaABC1D

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-haA3A-CBE-A

Plasmid

#220892

Purpose

Express haA3A-CBE-A (with SpCas9) in mammalian cells

Depositor

Dali Li

Article

Yang et al Nat Chem Biol. 2024 Mar 29. doi: 10.1038

Insert

haA3A-CBE-A

Expression

Mammalian

Available Since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_UCN1.0

Plasmid

#208669

Purpose

Expresses the genetically-encoded fluorescent urocortin (UCN) sensor GRAB_UCN1.0 in a cre-dependent manner

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based urocortin (UCN) sensor GRAB_UCN1.0

Use

AAV

Promoter

hSyn

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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RCAS-Flag-BRAFV600E

Plasmid

#126615

Purpose

Retroviral expression of Flag tagged human oncogenic BRAFV600E

Depositor

Yi Li

Article

Bu et al Cancer Res. 2019 Jan 1;79(1):61-71. doi:

Insert

BRAF (BRAF Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

V600E

Available Since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-HkCas12a-2AeGFP

Plasmid

#123634

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

Insert

HkCas12a

Tags

3xHA tag

Expression

Mammalian

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-fDIO-GRAB_eCB2.0

Plasmid

#164610

Purpose

Express the endocannabinoid sensor GRAB_eCB2.0 in FLP positive cells

Depositor

Yulong Li

Article

Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

Insert

Endocannabinoid sensor GRAB_eCB2.0

Use

AAV

Mutation

S383T

Promoter

EFS

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-DIO-GRAB_Ado1.0med

Plasmid

#153292

Purpose

Express the medium-affinity adenosine sensor GRAB_Ado1.0med in Cre positive cells

Depositor

Yulong Li

Article

Wu et al Proc Natl Acad Sci U S A. 2023 Apr 4;120

Insert

Adenosine sensor GRAB_Ado1.0med (medium affinity)

Use

AAV

Promoter

EFS

Available Since

July 15, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-GRAB_eCB2.0mut

Plasmid

#164607

Purpose

Express the endocannabinoid non-responsive sensor GRAB_eCB2.0 in Cre positive neurons

Depositor

Yulong Li

Article

Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

Insert

Endocannabinoid non-responsive sensor GRAB_eCBmut

Use

AAV

Mutation

S383T, F177A

Promoter

hSyn

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti AID-BEmax

Plasmid

#157950

Purpose

Lentiviral expression of AID-BEmax

Depositor

Dali Li

Article

Zhang et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

Lenti AID-BEmax

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pTXBI-mClover-YTH domain

Plasmid

#177123

Purpose

Bacterial expression of YTH domain of YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purification

Depositor

Wenbo Li

Article

Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

Insert

YTHDC1 (YTH domain) (YTHDC1 Human)

Tags

Mxe GyrA intein-Chitin binding domain for protein…

Expression

Bacterial

Mutation

YTH domain only (IDR1 and IDR2 deletion)

Promoter

T7

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB_CCK1.0

Plasmid

#208672

Purpose

Expresses the genetically-encoded fluorescent cholecystokinin (CCK) sensor GRAB_CCK1.0 in mammalian cells

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based cholecystokinin (CCK) sensor GRAB_CCK1.0

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pCW57-mcGAS-LL/RK-NLS/D307A

Plasmid

#185459

Purpose

Inducible nuclear cGAS with D307A mutation

Depositor

Shitao Li

Article

Wu et al Commun Biol. 2022 May 10;5(1):433. doi:

Insert

cGAS

Use

Lentiviral

Tags

FLAG and SV40 NLS

Expression

Mammalian

Mutation

L157R, L159K, D307A

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-AaCas12b-2AeGFP

Plasmid

#121945

Purpose

Mammalian expression, Genome editing

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

AaCas12b

Tags

3xHA tag

Expression

Mammalian

Available Since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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pGEMT-PT2A-iRFP670-Tdtomato-GFP

Plasmid

#111817

Purpose

Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

Depositor

Li Qian

Article

Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

Inserts

iRFP670
Tdtomato
GFP

Use

Cloning intermediate

Available Since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pTXBI-mclover-YTHDC1-FL

Plasmid

#177121

Purpose

Bacterial expression of Full-length YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purification

Depositor

Wenbo Li

Article

Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

Insert

YTHDC1 (YTHDC1 Human)

Tags

Mxe GyrA intein-Chitin binding domain for protein…

Expression

Bacterial

Promoter

T7

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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