-
Plasmid#229700PurposeLentiviral expression of LifeAct-TagRFPT in mammalian cellsDepositorInsertSynthetic construct LifeAct-mTurquoise gene
UseLentiviralTagsTagRFPTExpressionMammalianMutationPromoterCMV promoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pC1-lifeact-oROS-HT
Plasmid#216420PurposeTargeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to actin filaments.DepositorInsertlifeact-oROS-HT
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
LifeAct-SNAPf-mTurquoise2
Plasmid#197497PurposeExpressing fluorescent SNAPf-mTurquoise2 fusion protein in mammalian cells with localization in actin filaments.DepositorInsertSNAPf
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFX-Lifeact-EGFP
Plasmid#174477PurposeExpress EGFP with actin-targeting sequence in mammalian cellsDepositorInsertEGFP with actin-targeting sequence
UseTagsLifeact (N-terminal 17 amino acids from S. Cerevi…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFX-Lifeact-SECFP
Plasmid#174476PurposeExpress SECFP with actin-targeting sequence in mammalian cellsDepositorInsertSECFP with actin-targeting sequence
UseTagsLifeact (N-terminal 17 amino acids from S. Cerevi…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFX-Lifeact-Sirius
Plasmid#174475PurposeExpress Sirius with actin-targeting sequence in mammalian cellsDepositorInsertSirius with actin-targeting sequence
UseTagsLifeact (N-terminal 17 amino acids from S. Cerevi…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
Lifeact-mEos2-APEX2
Plasmid#115563PurposeLifeact-mEos2-APEX2 fusion for PALM and CLEM. Parton lab clone IETDepositorInsertmEos2
UseTagsAPEX2 and LifeactExpressionMammalianMutationPromoterAvailable sinceSept. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAFNF-LifeAct-EGFP
Plasmid#163608PurposeExpresses LifeAct fused to EGFP Flp-dependently in mammalian cellsDepositorInsertLifeAct
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
LifeAct-mTq2 pDRF1-GW
Plasmid#168084PurposeExpresses Lifeact-mTq2 to visualize actin in yeastDepositorInsertLifeAct-mTq2
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
LifeAct-mScarlet pDRF1-GW
Plasmid#168086PurposeExpresses Lifeact-mScarlet to visualize actin in yeastDepositorInsertLifeAct-mScarlet
UseTagsExpressionYeastMutationPromoterAvailable sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
mGeos2-VESS-Lifeact-7
Plasmid#54526PurposeLocalization: Actin, Excitation: 503 / 569, Emission: 514 / 581DepositorTypeEmpty backboneUseTagsmGeos2-VESS-LifeactExpressionMammalianMutationPromoterAvailable sinceJuly 2, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
mGeos2-VEMS-Lifeact-7
Plasmid#54818PurposeLocalization: Actin, Excitation: 503 / 569, Emission: 514 / 581DepositorTypeEmpty backboneUseTagsmGeos2-VEMS-LifeactExpressionMammalianMutationPromoterAvailable sinceJune 20, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEGFP-C1 Lifeact-EGFP-2XNLS
Plasmid#58467PurposeExpresses a nuclear-targeted actin filament reporter, consisting of the peptide Lifeact, on a CMV promoterDepositorInsertsLifeact
2X NLS with 2X stop codons
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti Lifeact-EGFP BlastR
Plasmid#84383PurposeLentiviral expression of EGFP-tagged Lifeact with Blasticidin selection in cells including neuronsDepositorInsertLifeact
UseLentiviralTagsEGFPExpressionMutationPromoterCMV immediate earlyAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti Lifeact-mTagBFP2 PuroR
Plasmid#101893PurposeLentiviral expression of mTagBFP2-tagged Lifeact with Puromycin selection in cells including neuronsDepositorInsertLifeact
UseLentiviralTagsmTagBFP2ExpressionMutationPromoterCMV immediate earlyAvailable sinceMay 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLenti Lifeact-iRFP670 BlastR
Plasmid#84385PurposeLentiviral expression of iRFP670-tagged Lifeact with Blasticidin selection in cells including neuronsDepositorInsertLifeact
UseLentiviralTagsiRFP670ExpressionMutationPromoterCMV immediate earlyAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLenti Lifeact-mRuby2 BlastR
Plasmid#84384PurposeLentiviral expression of mRuby2-tagged Lifeact with Blasticidin selection in cells including neuronsDepositorInsertLifeact
UseLentiviralTagsmRuby2ExpressionMutationPromoterCMV immediate earlyAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIRES LifeAct-GFP puro3
Plasmid#64357PurposeBicistronic expression of LifeAct-GFP fusion and puro3 resistance geneDepositorInsertLifeAct-GFP
UseTagsIRES-PuroExpressionMammalianMutationPromoterCMVAvailable sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti Lifeact-mRuby2 PuroR
Plasmid#84386PurposeLentiviral expression of mRuby2-tagged Lifeact with Puromycin selection in cells including neuronsDepositorInsertLifeact
UseLentiviralTagsmRuby2ExpressionMutationPromoterCMV immediate earlyAvailable sinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIRES Hygro3 LifeAct-mChe
Plasmid#64343PurposeBicistronic expression of LifeAct-mCherry fusion and Hygro resistance geneDepositorInsertLifeAct
UseTagsIRES-Hygro and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only