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Plasmid#135919PurposeEncodes ABCC8 exon 27 wild type for the analysis of splicing variantsDepositorInsertATP-binding Cassette subfamily C member 8 (ABCC8 Human)
UseExon trappingTagsExpressionMammalianMutationPromoterSV40Available sinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCSmEn2n4ERT(#234)
Plasmid#184082Purposecyclofen-inducible chicken EN2 (mut) activation via mammalian cell transfection or mRNA synthesisDepositorInsert6his-myc-chkEn2(n4)-ERT2
UseSynthetic BiologyTags6his-MycExpressionMammalianMutationchkEn2: G238S; ERT2: G400V, M543A, L544A, deleted…PromotersCMV+SP6Available sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCSmEn2SRERT(#231)
Plasmid#184080Purposecyclofen-inducible chicken EN2 (mut) activation via mammalian cell transfection or mRNA synthesisDepositorInsert6his-myc-chkEn2(SR)-ERT2
UseSynthetic BiologyTags6his-MycExpressionMammalianMutationchkEn2: W247S, F248R; ERT2: G400V, M543A, L544A, …PromotersCMV+SP6Available sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22UbhaEng2aE4PCh(#271)
Plasmid#184085Purposecyclofen-inducible fish Eng2a (wt) activation in zebrafish permanent transgenicDepositorInsert3HA-ZfEng2a-ERT2-P2A-mCherry
UseZebrafish transgenesis (blue eyes)Tags3xHAExpressionMutationERT2: G400V, M543A, L544A, deleted 1-281;PromoterZf-UbiAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22UbhaEng2bE4PCh(#272)
Plasmid#184086Purposecyclofen-inducible fish Eng2b (wt) activation in zebrafish permanent transgenicDepositorInsert3HA-ZfEng2b-ERT2-P2A-mCherry
UseZebrafish transgenesis (blue eyes)Tags3xHAExpressionMutationERT2: G400V, M543A, L544A, deleted 1-281;PromoterZf-UbiAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22gCfpUbm5En2E4PCh(#251)
Plasmid#184083Purposecyclofen-inducible chicken EN2 (wt) activation in zebrafish permanent transgenicDepositorInsert5myc-chkEn2-ERT2-P2A-mCherry
UseZebrafish transgenesis (blue eyes)Tags5xMycExpressionMutationERT2: G400V, M543A, L544A, deleted 1-281;PromoterZf-UbiAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22Ubm5Eng2bE4PCh(#284)
Plasmid#184088Purposecyclofen-inducible fish Eng2b (wt) activation in zebrafish permanent transgenicDepositorInsert5myc-ZfEng2b-ERT2-P2A-mCherry
UseZebrafish transgenesis (blue eyes)Tags5xMycExpressionMutationERT2: G400V, M543A, L544A, deleted 1-281;PromoterZf-UbiAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22UbHEn2E4PCh(#262)
Plasmid#184084Purposecyclofen-inducible chicken EN2 (wt) activation in zebrafish permanent transgenicDepositorInsert3HA-chkEn2-ERT2-P2A-mCherry
UseZebrafish transgenesis (blue eyes)Tags3xHAExpressionMutationERT2: G400V, M543A, L544A, deleted 1-281;PromoterZf-UbiAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT22Ubm5Eng2aE4PCh(#283)
Plasmid#184087Purposecyclofen-inducible fish Eng2a (wt) activation in zebrafish permanent transgenicDepositorInsert5myc-ZfEng2a-ERT2-P2A-mCherry
UseZebrafish transgenesis (blue eyes)Tags5xMycExpressionMutationERT2: G400V, M543A, L544A, deleted 1-281;PromoterZf-UbiAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCSmEn25EERT(#232)
Plasmid#184081Purposecyclofen-inducible chicken EN2 (mut) activation via mammalian cell transfection or mRNA synthesisDepositorInsert6his-myc-chkEn2(5E)-ERT2
UseSynthetic BiologyTags6his-MycExpressionMammalianMutationchkEn2: S150E, S151E, S153E, S155E, S156E; ERT2: …PromotersCMV+SP6Available sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-NTOM20-SbMVpC-43LK-iLID(139)-NES-NES-SbMVpN-iLID-SbMVseq-bArr2-TevCs-P2A-EGFP
Plasmid#210505Purposeexpresses NTOM20-SbMVpC-43LK-iLID(139)-NES-NES-SbMVpN-iLID-SbMVseq-bArr2-TevCs-P2A-EGFP component in mammalian cellsDepositorInsertNTOM20-SbMVpC-43LK-iLID(139)-NES-NES-SbMVpN-iLID-SbMVseq-bArr2-TevCs-P2A-EGFP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-NLSx2-TVMVpC-43LK-iLID(139)-NES-NES-TVMVpN-iLID-TVMVseq-Src13-GB1-LRP6c
Plasmid#210508Purposeexpresses NLSx2-TVMVpC-43LK-iLID(139)-NES-NES-TVMVpN-iLID-TVMVseq-Src13-GB1-LRP6c component in mammalian cellsDepositorInsertNLSx2-TVMVpC-43LK-iLID(139)-NES-NES-TVMVpN-iLID-TVMVseq-Src13-GB1-LRP6c
UseTagsGB1ExpressionMammalianMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-NTOM20-EGFP-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq--bArr2-TevCs
Plasmid#210516Purposeexpresses NTOM20-EGFP-pCMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq--bArr2-TevCs component in mammalian cellsDepositorInsertNTOM20-EGFP-pCMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq--bArr2-TevCs
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT22Ubm5En2SRE4PCh(#302)
Plasmid#184089Purposecyclofen-inducible chicken EN2 (mut) activation in zebrafish permanent transgenicDepositorInsert5myc-chkEn2(SR)-ERT2-P2A-mCherry
UseZebrafish transgenesis (blue eyes)Tags5xMycExpressionMutationchkEn2: W247S, F248R; ERT2: G400V, M543A, L544A, …PromoterZf-UbiAvailable sinceJune 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetR-FKBP-SbMVseq-SbMVp-SbMVseq-FRB-VP16
Plasmid#210506Purposeexpresses PDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetR-FKBP-SbMVseq-SbMVp-SbMVseq-FRB-VP16 component in mammalian cellsDepositorInsertPDGFR-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetR-FKBP-SbMVseq-SbMVp-SbMVseq-FRB-VP16
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
hSyn-PDGFR-KA2-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetR-FKBP
Plasmid#210509Purposeexpresses PDGFR-KA2-TevCs-43LK-iLID(139)-NES-NES-TevN-iLID-TEVseq-TetR-FKBP component in rat hippocampal neuron cellsDepositorInsertsUseTagsMycExpressionMammalianMutationPromoterhSynAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28 E2 LBD-Tev-E1a-His
Plasmid#232117PurposeFor bacterial expression of a fusion protein containing from N to C-terminus: the lipoyl-binding domain of Human E2, a TEV protease site, a peptide from Human E1a, and a His tag.DepositorInsertDBT (DBT Human)
UseTags6xHis, RIGHHSTSDDSSAY (AA331 to 345 (preprocessin…ExpressionBacterialMutationPromoterT7 PromoterAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST (AAV9)
Viral Prep#105448-AAV9PurposeReady-to-use AAV9 particles produced from pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST (#105448). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-ChrimsonR-mRuby2-ST plasmid DNA. hSyn-driven expression of ChrimsonR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites for optogenetic activation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsmRuby2Available sinceSept. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_OT1.0 (AAV1)
Viral Prep#185386-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-hSyn-GRAB_OT1.0 (#185386). In addition to the viral particles, you will also receive purified pAAV-hSyn-GRAB_OT1.0 plasmid DNA. hSyn-driven expression of the genetically-encoded fluorescent oxytocin(OT) sensor GRAB_OT1.0 in neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceJune 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_OTmut (AAV1)
Viral Prep#185388-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-hSyn-GRAB_OTmut (#185388). In addition to the viral particles, you will also receive purified pAAV-hSyn-GRAB_OTmut plasmid DNA. hSyn-driven expression of the genetically-encoded fluorescent oxytocin(OT) control sensor GRAB_OTmut in neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsNoneAvailable sinceOct. 21, 2024AvailabilityAcademic Institutions and Nonprofits only