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Showing: 241 - 260 of 9391 results

  5. pUC19-U6-BsmBI_epegRNA_entry-mpknot_(LM1140)

    Plasmid
    #228253
    Purpose
    pU6 entry plamid for cloning prime editor mpknot epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)
    Depositor
    Benjamin Kleinstiver
    Article
    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586
    Insert
    mpknot entry plasmid backbone for epegRNA expression via human U6 promoter
    Use
    Tags
    Expression
    Mammalian
    Mutation
    n/a
    Promoter
    U6
    Available since
    April 23, 2025
    Availability
    Academic Institutions and Nonprofits only
    228253-plasmid-map-sequence-id-451024

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  6. hSLC17A6/vGlutT2/pCMV6-entry

    Plasmid
    #196939
    Purpose
    Plasmid expressing an antigen targeting the VGluT2 transporter
    Depositor
    James Trimmer
    Insert
    vGlutT2/ SLC17A6
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    196939-plasmid-map-sequence-id-395174

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  7. pMS22: pHelper(PmcCAST)_entry_ΔTniQ

    Plasmid
    #168155
    Purpose
    Inducible expression of PmcCAST proteins (except TniQ). Two BsaI sites for spacer cloning.
    Depositor
    Feng Zhang
    Article
    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.
    Inserts
    PmcCAST minimal CRISPR array
    PmcCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
    PmcCAST Tns proteins (TnsAB, TnsC and TnsD)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    168155-plasmid-map-sequence-id-330972

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  8. pMS5: pHelper(AvCAST)_entry_ΔTniQ

    Plasmid
    #168138
    Purpose
    Inducible expression of AvCAST proteins (except TniQ). Two BsaI sites for spacer cloning.
    Depositor
    Feng Zhang
    Article
    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.
    Inserts
    AvCAST minimal CRISPR array
    AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
    AvCAST Tns proteins (TnsA, TnsB and TnsC)
    AvTnsD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    168138-plasmid-map-sequence-id-330692

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  9. pMS4: pHelper(AvCAST)_entry

    Plasmid
    #168137
    Purpose
    Inducible expression of AvCAST proteins. Two BsaI sites for spacer cloning.
    Depositor
    Feng Zhang
    Article
    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.
    Inserts
    AvCAST minimal CRISPR array
    AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
    AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
    AvTnsD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    168137-plasmid-map-sequence-id-330973

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  18. pJRH-1362 scFv entry plasmid

    Plasmid
    #201912
    Purpose
    Entry vector for cloning single chain variable fragments displayed on the human CD8a hinge and transmembrane domain (CAG promoter)
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103
    Insert
    Stuffer sequence (drop out for scFv cloning) + CD8 hinge and transmembrane domain
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Aug. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    201912-plasmid-map-sequence-id-403308

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  19. pShHELIX(Cas12k-TnsC)-sgRNA_entry (CJT113)

    Plasmid
    #181792
    Purpose
    Expresses 3-component ShHELIX containing a Cas12k-TnsC fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShTnsB, ShTniQ, ShCas12k-ShTnsC
    Use
    Tags
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    181792-plasmid-map-sequence-id-358226

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Showing: 241 - 260 of 9391 results