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pW5Y_Apr

Plasmid

#158211

Purpose

BAC-based, lacUV5 regulon and Cre recombinase in loxP and lox5171 sites

Depositor

Yasuo Yoshikuni

Article

Wang et al Nat Microbiol. 2019 Dec;4(12):2498-2510.

Type

Empty backbone

Available Since

Feb. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Strep-FLAG KBP

Plasmid

#236459

Purpose

transient overexpression of KBP in mammalian cells

Depositor

Michele Pagano

Article

Donato et al Nat Cell Biol. 2017 Apr;19(4):341-351. d

Insert

KBP (KIF1BP Human)

Tags

Strep-FLAG

Expression

Mammalian

Available Since

May 19, 2025

Availability

Academic Institutions and Nonprofits only

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pdCas9-GFP

Plasmid

#181906

Purpose

Fluorescently tagged dead Cas9

Depositor

Karsten Rippe

Article

Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

Insert

dCas9

Use

CRISPR

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pGEM-HSPA5P

Plasmid

#44150

Purpose

a fragment containing 429 bp of the human HSPA5 promoter (-282 to +147)

Depositor

Joan Conaway

Article

Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

Insert

HSPA5 promoter (HSPA5 Human)

Expression

Mammalian

Available Since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only
pET28_6xHis-PARP1-CAT

Plasmid

#173943

Purpose

Bacterial expression of isolated PARP1 CAT domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-CAT (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-flex-Voltron

Plasmid

#119035

Purpose

Cre-dependant expression of Voltron in neurons

Depositor

Eric Schreiter

Article

Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

Insert

Voltron

Use

AAV

Expression

Mammalian

Promoter

hsyn

Available Since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/Flag-FLJ10081

Plasmid

#64775

Purpose

Mammalian expression of Flag-tagged FLJ10081 (NSL3)

Depositor

Joan Conaway, Ronald Conaway

Article

Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

Insert

FLJ10081 (KANSL3 Human)

Tags

Flag

Expression

Mammalian

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA5-Flag-KIAA0947

Plasmid

#49248

Purpose

Mammalian expression of flag-tagged human KIAA0947

Depositor

Joan Conaway, Ronald Conaway

Article

Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

Insert

KIAA0947 (ICE1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only
pET28_6xHis-PARP1delBRCT

Plasmid

#173938

Purpose

Bacterial expression of PARP1 lacking BRCT domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delBRCT (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 374-520; V762A

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-Zn2

Plasmid

#174805

Purpose

Bacterial expression of isolated PARP1 Zn2 domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-Zn2 (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-Zn3

Plasmid

#174806

Purpose

Bacterial expression of isolated PARP1 Zn3 domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-Zn3 (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 2xSTREP Cyclin F(LP35/36AA)

Plasmid

#236467

Purpose

transient overexpression of Cyclin F in mammalian cells

Depositor

Michele Pagano

Article

D'Angiolella et al Cell. 2012 May 25;149(5):1023-34. doi: 1

Insert

Cyclin F (CCNF Human)

Tags

2xSTREP

Expression

Mammalian

Mutation

(LP35/36AA) first two amino acids of the F-box do…

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1delZn2

Plasmid

#173935

Purpose

Bacterial expression of PARP1 lacking Zn2 domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delZn2 (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 97-213 (V762A)

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT-Flag-Cul5

Plasmid

#31984

Purpose

Mammalian expression of Flag-tagged Cul5

Depositor

Joan Conaway

Article

Mahrour et al J Biol Chem. 2008 Mar 21;283(12):8005-13

Insert

Cul5 (CUL5 Human)

Tags

Flag

Expression

Mammalian

Available Since

June 10, 2013

Availability

Academic Institutions and Nonprofits only
pET28_6xHis-PARP1delCAT

Plasmid

#173942

Purpose

Bacterial expression of PARP1 lacking CAT domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delCAT (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 661-1014

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1delWGR

Plasmid

#173940

Purpose

Bacterial expression of PARP1 lacking WGR domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delWGR (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 521-660; V762A

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT-Flag-NRBP1

Plasmid

#48197

Purpose

Expressed flag-tagged NRBP1 (nuclear receptor binding protein 1)

Depositor

Joan Conaway, Ronald Conaway

Article

Mahrour et al J Biol Chem. 2008 Mar 21;283(12):8005-13

Insert

NRBP1 (NRBP1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only
pET28_6xHis-PARP1delZn3

Plasmid

#173936

Purpose

Bacterial expression of PARP1 lacking Zn3 domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delZn3 (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 214-373; V762A

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1delZn1

Plasmid

#173933

Purpose

Bacterial expression of PARP1 lacking Zn1 domain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delZn1 (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 1-96; V762A

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-HD

Plasmid

#174809

Purpose

Bacterial expression of isolated PARP1 HD subdomain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-HD (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

V762A

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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