We narrowed to 152,655 results for: des
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pCA24N-ligase-pprA
Plasmid#87744PurposeIPTG-inducible expression of ligase-pprA fusion for protein purificationDepositorInsertT4 DNA ligase (30 )
UseTags6xHis and codon optimized pprA (from Deinococcus …ExpressionBacterialMutationPromoterT5-lacAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAN1935
Plasmid#127203PurposeConstitutive expression of Venus-degronSwitchA and mScarlet-degronSwitchCDepositorInsertpTEF1-Venus-degronSwitch_A_t12-tENO2-pTEF2-mScarlet-Linker-degronSwitch_C-tPGK1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMay 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRSET a sfMatryoshCaMP6s
Plasmid#100020PurposeBacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting superfolder cpGFP.DepositorInsertsfMatryoshCaMP6s
UseTags6x HIS tag and Xpress_EK tagExpressionBacterialMutationcpEGFP replaced with superfolder cpGFPPromoterT7Available sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAN1232
Plasmid#127164PurposeVector for integrating the controller (constitutive expression of GEM-degronSwitch and pZ3 expression of key)DepositorInsertpRPL18B-GEM-degronSwitchA-tENO2-pZ3-keyA-mTurquoise2-NLS(SV40)-tTDH1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
ABE8e(TadA-8e V82G)
Plasmid#138494PurposeA-to-G base editorDepositorInsertecTadA(8e V82G)-nSpCas9
UseTagsExpressionMammalianMutationTadA-8e V82GPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
(PM-S608E)FLAG-Cep215-FL
Plasmid#106906PurposeExpresses murine phospho-mimetic CEP215 at S608DepositorInsertCEP215 (Cdk5rap2 Mouse)
UseTagsFLAGExpressionMammalianMutationchanged Serine-608 to Glutamic AcidPromoterCMVAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-h-mmPtch1-B-HA
Plasmid#120902PurposeExpresses Ptch1-B for function assaysDepositorInsertptch1 (Ptch1 Mouse)
UseTagsHAExpressionMammalianMutationdeleted amino acids 620-643, truncated at 1291PromoterCMVAvailable sinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAN1572
Plasmid#127202PurposeDual inducible expression of Venus-degronSwitchA and KeyA-BFPDepositorInsertpGal1-Venus-degronSwitch_A_t12-tSSA1-pZ3-key_A_e18-mTagBFP2-tENO1
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD003 sgLacZ-1
Plasmid#74968PurposesgLacZ-1, puromycin selectionDepositorInsertLacZ
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD003 sgLacZ-2
Plasmid#74969PurposesgLacZ-2, puromycin selectionDepositorInsertLacZ
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCLX-MIFE-hM3Dq-PGK-mCherry
Plasmid#114294PurposeMifepristone-inducible expression of hM3Dq proteinDepositorInserthM3Dq
UseLentiviralTagsExpressionMutationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
CSII-IDR2-chimpTRIM5-FOS
Plasmid#79065PurposeExpress chimpanzee TRIM5alpha in mammalan cellsDepositorInsertTRIM5alpha(chimpanzee)-FOS
UseTagsOneSTrEP-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-Kozak-rhTRIM5alpha-PFOS
Plasmid#79038PurposeBaculoviral entry vector to produce Bac-to-bac baculovirusesDepositorInsertrhesus TRIM5alha-PFOS
UseTagsOneSTrEP-FLAGExpressionInsectMutationPromoterpolyhedrinAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Pchi-HMG
Plasmid#231540PurposeExpresses the HMG of Pchi Sox with a 6xHis tag for protein purificationDepositorInsertSox HMG
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceMarch 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Salhel-HMG
Plasmid#231539PurposeExpresses the HMG of Salhel Sox-I with a 6xHis tag for protein purificationDepositorInsertSox-I HMG
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceMarch 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Myflu-POU
Plasmid#231542PurposeExpresses the DNA binding domain of Myflu POU with a 6xHis tag for protein purificationDepositorInsertPOU DBD
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceMarch 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Salhel-POU
Plasmid#231541PurposeExpresses the DNA binding domain of Salhel POU with a 6xHis tag for protein purificationDepositorInsertPOU DBD
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceMarch 18, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetO-Myflu-POU
Plasmid#231523PurposeExpresses Mylnosiga fluctuans full-length POU in mammalian cells, for lentivirus generationDepositorInsertPOU
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETG20a-Salro-HMG
Plasmid#231543PurposeExpresses the HMG of Salro Sox-like, for protein purificationDepositorInsertSox-Iike HMG
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETG20a-hCic-HMG
Plasmid#231545PurposeExpresses the HMG of human Cic, for protein purificationDepositorInsertCic HMG
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETG20a-Pchi-HMG
Plasmid#231538PurposeExpresses the HMG of Pchi Sox, for protein purificationDepositorInsertSox HMG
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETG20a-Monbr-HMG
Plasmid#231544PurposeExpresses the HMG of Monbr Sox-like, for protein purificationDepositorInsertSox-Iike HMG
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pETG20a-Salhel-HMG
Plasmid#231537PurposeExpresses the HMG of Salhel Sox-I, for protein purificationDepositorInsertSox-I HMG
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetO-Sox2-HMG
Plasmid#231532PurposeExpresses mouse Sox2 HMG in mammalian cells, for lentivirus generationDepositorInsertmSox2 HMG
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetO-Salhel-POU
Plasmid#231522PurposeExpresses Salpingoeca helianthica full-length POU in mammalian cells, for lentivirus generationDepositorInsertPOU
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCas3-APOBEC1
Plasmid#229536PurposepMV_hyg encoding Cas3(wt)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloningDepositorInsertsrAPOBEC1
Cas3
Uracil glycosylase inhibitor
UseTagsXTENExpressionBacterial and YeastMutationcodon optimized for S.cerevisiae and codon optimi…PromoterAvailable sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCas3-CDA1
Plasmid#229534PurposepMV_hyg encoding Cas3(wt)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloningDepositorInsertsCas3
Cytidine deaminase
Uracil glycosylase inhibitor
UseTagsXTENExpressionBacterial and YeastMutationcodon optimized for S.cerevisiae and codon optimi…PromoterAvailable sinceJan. 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
EYFP-Parkin
Plasmid#210292Purposemitophagy markerDepositorInsertEYFP-Parkin
UseTagsEYFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
mActA-FKBP-mCherry
Plasmid#210295PurposeActuAtor for FKBP-FRB CIDDepositorInsertmActA-FKBP-mCherry
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
EYFP-FKBP-N-WASP
Plasmid#210302Purposetargeted actin nucleationDepositorInsertEYFP-FKBP-N-WASP
UseTagsEYFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR223-BPLF1_2001-3000
Plasmid#198586PurposeGateway Entry CloneDepositorInsertBPLF1_2001-3000
UseGateway entry vectorTagsExpressionMutationNone (wildtype)PromoterAvailable sinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCit(P5f-P13f)3-gs
Plasmid#228080PurposeMammalian expression of coiled coil segments (P5f-P13f)x3, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.DepositorInsertmCit, P5f, P13f
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCit(S1h)3(S3h)3-gs
Plasmid#228079PurposeMammalian expression of coiled coil segments (S1h)x3-(S3h)x3, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.DepositorInsertmCit, S1h, S3h
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
TagBFP(S2-S4)1-gs
Plasmid#228074PurposeMammalian expression of coiled coil segments (S2-S4)x1, with short flexible linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells.DepositorInsertBFP, S2, S4
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
TagBFP(S2)3(S4)3-SAH
Plasmid#228069PurposeMammalian expression of coiled coil segments (S2)x3-(S4)x3, with long rigid linkers and N-terminal TagBFP; for phase separation and forming of liquid condensates in mammalian cells.DepositorInsertBFP, S2, S4
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCit(S1h-S3h)3-gs
Plasmid#228076PurposeMammalian expression of coiled coil segments (S1h-S3h)x3, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.DepositorInsertmCit, S1h, S3h
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCit(D1h-D3h)3-gs
Plasmid#228088PurposeMammalian expression of coiled coil segments (D1h-D3h)x3, with short flexible linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.DepositorInsertmCit, D1h, D3h
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCit(S1-S3)1-SAH
Plasmid#228073PurposeMammalian expression of coiled coil segments (S1-S3)x1, with long rigid linkers and N-terminal mCitrine; for phase separation and forming of liquid condensates in mammalian cells.DepositorInsertmCitrine, S1, S3
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
TagBFP(P6f)3(P14f)3-gs
Plasmid#228081PurposeMammalian expression of coiled coil segments (P6f)x3-(P14f)x3, with short flexible linkers and N-term TagBFP; for phase separation and forming of liquid condensates in mammalian cells.DepositorInsertBFP, P6f, P14f
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 6, 2024AvailabilityAcademic Institutions and Nonprofits only
