We narrowed to 7,410 results for: 11

1. pFM114

   Plasmid

   #40160

   Depositor

   Geraldine Seydoux

   Article

   Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

   Insert

   par-3N+S251A (par-3 Nematode)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   N terminal portion of protein (aa 1-364); S251A

   Promoter

   tac

   Available Since

   Oct. 23, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pFM116

   Plasmid

   #40162

   Depositor

   Geraldine Seydoux

   Article

   Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

   Insert

   par-3C+S950A (par-3 Nematode)
   

   Tags

   GST

   Expression

   Bacterial

   Mutation

   C terminal portion of protein (aa840-1268); S950A

   Promoter

   tac

   Available Since

   Sept. 12, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pSL7 SMR1791

   Plasmid

   #28030

   Depositor

   Susan Rosenberg

   Article

   Harris et al Genes Dev. 1997 Sep 15. 11(18):2426-37.

   Insert

   MutS, MutL (mutL, mutS E. coli)
   

   Expression

   Bacterial

   Available Since

   April 1, 2011

   Availability

   Academic Institutions and Nonprofits only

4. pWZ414-F48

   Plasmid

   #23072

   Depositor

   Sharon Dent

   Article

   Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

   Insert

   yeast Histone H3-2 and Histone H4-2
   

   Expression

   Yeast

   Mutation

   Histone H3 changed Lysine 14 to Glutamine, H3 K14…

   Available Since

   March 3, 2010

   Availability

   Academic Institutions and Nonprofits only

5. pWZ414-F50

   Plasmid

   #23074

   Depositor

   Sharon Dent

   Article

   Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

   Insert

   yeast Histone H3-2 and Histone H4-2
   

   Expression

   Yeast

   Mutation

   Histone H3 changed Lysine 14 to Arginine, H3 K14R…

   Available Since

   Feb. 17, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pWZ414-F54

   Plasmid

   #23078

   Depositor

   Sharon Dent

   Article

   Zhang et al EMBO J. 1998 Jun 1. 17(11):3155-67.

   Insert

   yeast Histone H3-2 and Histone H4-2
   

   Expression

   Yeast

   Mutation

   Histone H3 changed Lysine 9 to Glutamine, H3 K9Q …

   Available Since

   Feb. 17, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pES1004

   Plasmid

   #21790

   Depositor

   Richard Yu

   Article

   Yu et al Nature. 2008 Dec 11. 456(7223):755-61.

   Insert

   Kss1-as2-trunc (KSS1 Budding Yeast)
   

   Expression

   Yeast

   Mutation

   deleted bases 1-14 from open reading frame E94A …

   Available Since

   Oct. 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. pILGFP3AE4

   Plasmid

   #185873

   Purpose

   A plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (~11 copy)

   Depositor

   Claudia Vickers

   Type

   Empty backbone

   Expression

   Yeast

   Mutation

   None

   Available Since

   Sept. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSpCas9(BB)-2A-GFP (PX458)

   Plasmid

   #48138

   Purpose

   Cas9 from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Has Service

   Cloning Grade DNA

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. lentiGuide-Puro

    Plasmid

    #52963

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

    Has Service

    Cloning Grade DNA

    Inserts

    S. pyogenes sgRNA cassette
    Puromycin resistance
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    Ef1-a and hU6

    Available Since

    May 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKMD106h - intein-Cas9(C574)

    Plasmid

    #64191

    Purpose

    Expresses intein-Cas9(C574) in mammalian cells

    Depositor

    David Liu

    Article

    Davis et al Nat Chem Biol. 2015 May;11(5):316-8. doi

    Insert

    mammalian codon-optimized S. pyogenes intein-Cas9(C574) - NLS - 3x FLAG
    

    Use

    CRISPR

    Tags

    NLS - 3x FLAG (C terminal on insert)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3 HA human NEMO

    Plasmid

    #13512

    Depositor

    Kunliang Guan

    Article

    Tang et al J Biol Chem. 1999 Jun 11. 274(24):16741-

    Insert

    NEMO (IKBKG Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSpCas9n(BB)-2A-GFP (PX461)

    Plasmid

    #48140

    Purpose

    Cas9n (D10A nickase mutant) from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA

    Depositor

    Feng Zhang

    Article

    Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

    Insert

    hSpCas9n
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Mutation

    Cas9 D10A nickase mutant

    Promoter

    Cbh

    Available Since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLX303-ZIM3-KRAB-dCas9

    Plasmid

    #154472

    Purpose

    Expresses ZIM3 KRAB domain fused to the N terminus of dCas9

    Depositor

    Mikko Taipale

    Article

    Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

    Insert

    ZIM3 (ZIM3 Synthetic)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Includes ZIM3 aa 1-100

    Available Since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDF0112 cmv-cluciferase-polya-ef1a-g-luciferase-polya

    Plasmid

    #186980

    Purpose

    mammalian expression of Cluc-Gluc reporter

    Depositor

    Omar Abudayyeh, Jonathan Gootenberg

    Article

    Kato et al Cell. 2022 May 25. pii: S0092-8674(22)00

    Insert

    Cluc, Gluc
    

    Use

    Luciferase

    Available Since

    Aug. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLX303-ABI1-dCas9

    Plasmid

    #154474

    Purpose

    Expresses ABI1 fused to the N terminus of dCas9

    Depositor

    Mikko Taipale

    Article

    Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

    Insert

    ABI1 (ABI1 Mustard Weed)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Includes ABI1 aa 126-423, D143A

    Promoter

    CMV

    Available Since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJRH-1179 U6-reci Gag-Cas9 v2

    Plasmid

    #201914

    Purpose

    Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    Gag-Cas9 v2
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJRH-1180 U6-reci Gag-pol v2

    Plasmid

    #201915

    Purpose

    Cas9-EDV production plasmid. Expresses the Gag-pol (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

    Insert

    Gag-pol
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. Venus-rab11a

    Plasmid

    #234744

    Purpose

    Mammalian expression of recycling endosome marker Venus-rab11a

    Depositor

    Nevin Lambert

    Article

    Lan et al Traffic. 2012 Nov;13(11):1450-6. doi: 10

    Insert

    Venus-rab11a (RAB11A Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. Venus-rab7a

    Plasmid

    #234743

    Purpose

    Mammalian expression of late endosome marker Venus-rab7a

    Depositor

    Nevin Lambert

    Article

    Lan et al Traffic. 2012 Nov;13(11):1450-6. doi: 10

    Insert

    Venus-rab7a (Rab7 Mouse)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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