We narrowed to 4,523 results for: ARA-2

1. AAV-KrasG13A/sgKras/Cre

   Plasmid

   #99855

   Purpose

   Expresses Cre-recombinase, a barcoded HDR template to introduce a G13A mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

   Depositor

   Monte Winslow

   Article

   Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

   Insert

   Kras (Kras Mouse)
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Mutation

   AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

   Available Since

   May 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV-KrasG13R/sgKras/Cre

   Plasmid

   #99858

   Purpose

   Expresses Cre-recombinase, a barcoded HDR template to introduce a G13R mutation into the endogenous Kras locus, and a Kras-targeting sgRNA to enhance HDR.

   Depositor

   Monte Winslow

   Article

   Winters et al Nat Commun. 2017 Dec 12;8(1):2053. doi:

   Insert

   Kras (Kras Mouse)
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Mutation

   AvrII site (CAAAGG>CCTAGG), PAM/sgRNA mutation…

   Available Since

   Dec. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRSFDUET-1_VNp-anti-Lysozyme fAb-BiFC

   Plasmid

   #214950

   Purpose

   Bacterial expression of anti-chicken lysozyme Fab light and heavy chain VNp fusions. Each chain is fused to separate halves of a BiFC protein -Venus fluorescence signifies Hc/Lc heterodimer formation.

   Depositor

   Dan Mulvihill

   Article

   Baker et al Open Biol. 2025 Feb;15(2):240229. doi: 1

   Inserts

   VNp-anti-Lysozyme Fab heavy chain amino-BiFC fusion
   VNp-anti-Lysozyme Fab light chain amino-BiFC fusion
   

   Tags

   6xHis, Amino half of venus BIFC fragment, Carboxy…

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDiCre-mCherry

   Plasmid

   #164572

   Purpose

   Integration of a DiCre cassette in PbGFP parasite genome

   Depositor

   Olivier Silvie

   Article

   Fernandes et al PLoS One. 2020 Oct 12;15(10):e0236616. d

   Inserts

   FKBP12-CRE59
   FRB-CRE60
   mCherry
   Bifunctional dihydrofolate reductase-thymidylate synthase (TGME49_249180 Toxoplasma gondii)
   

   Use

   Cre/Lox

   Promoter

   Bidirectional PbEF1alpha promoter (PBANKA_1133300…

   Available Since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. CCR2-Tango

   Plasmid

   #66239

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   CCR2 (CCR2 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

6. GPR37L1-Tango

   Plasmid

   #66356

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   GPR37L1 (GPR37L1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

7. GAL-hAR-658-919

   Plasmid

   #89082

   Purpose

   mammalian expression of human androgen receptor ligand binding domain 658-919 amino acids linked to GAL4 DNA binding domain

   Depositor

   Elizabeth Wilson

   Article

   Askew et al J Biol Chem. 2007 Aug 31;282(35):25801-1

   Insert

   hAR-658-919 (AR Human)
   

   Tags

   GAL4 DBD

   Expression

   Mammalian

   Mutation

   aa 658-919

   Available Since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-15b-hAR-663-919

   Plasmid

   #89083

   Purpose

   bacterial expression of human androgen receptor ligand bindng domain amino acid residues 663-919 with histidine tag

   Depositor

   Elizabeth Wilson

   Article

   Askew et al J Biol Chem. 2007 Aug 31;282(35):25801-1

   Insert

   hAR ligand binding domain (AR Human)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   aa 663-919

   Promoter

   T7

   Available Since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. YFP-Parkin C431N

   Plasmid

   #46924

   Purpose

   expresses mutant Parkin with YFP tag in mammalian cells

   Depositor

   Richard Youle

   Article

   Lazarou et al J Cell Biol. 2013 Jan 21;200(2):163-72.

   Insert

   Park2 (PRKN Human)
   

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   changed cysteine 431 to asparagine

   Promoter

   CMV

   Available Since

   Aug. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTriEx4-ADCY6-C2 (917-1165 aa)

    Plasmid

    #113904

    Purpose

    His-S tagged cytoplasmic catalytic domain 2 of Adenylate Cyclase 6

    Depositor

    Anjaparavanda Naren

    Article

    Thomas et al J Biol Chem. 2018 Jun 14. pii: RA118.003

    Insert

    Adenylate Cyclase 6 cytoplasmic catalytic domain 2 (ADCY6 Human)
    

    Tags

    His-S

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Please see Depositor Comments

    Promoter

    T7, CMV

    Available Since

    Nov. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. Opto-GPR37L1

    Plasmid

    #106020

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR37L1; Opto-GPR37L1; B10)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR37L1 (GPR37L1 Human, Bovine)
    

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP-Drosha270-1374delNLS2

    Plasmid

    #62532

    Purpose

    N-terminal aa1-269 and nuclear localization signal 2 of Drosha are deleted

    Depositor

    Bharat Ramratnam

    Article

    Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

    Insert

    truncated human Drosha mutant (DROSHA Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-269 and nuclear localizatio…

    Available Since

    March 20, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pAc5.1B-EGFP-DmGW182_1-861-dsRNAres_R

    Plasmid

    #147401

    Purpose

    Insect Expression of DmGW182_1-861-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

    Insert

    DmGW182_1-861-dsRNAres (gw Fly)
    

    Expression

    Insect

    Mutation

    Two non silent mutations V704A and N799S and one …

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAc5.1B-EGFP-DmGW182-del205-490-dsRNAres_R

    Plasmid

    #147402

    Purpose

    Insect Expression of DmGW182-del205-490-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

    Insert

    DmGW182-del205-490-dsRNAres (gw Fly)
    

    Expression

    Insect

    Mutation

    Two non silent mutations V704A and N799S and one …

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAc5.1B-lambdaN-DmGW182-del205-490-dsRNAres_R

    Plasmid

    #147403

    Purpose

    Insect Expression of DmGW182-del205-490-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

    Insert

    DmGW182-del205-490-dsRNAres (gw Fly)
    

    Expression

    Insect

    Mutation

    Two non silent mutations V704A and N799S and one …

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAc5.1B-lambdaN-HA-DmGW182_1-861-dsRNAres_R

    Plasmid

    #147408

    Purpose

    Insect Expression of DmGW182_1-861-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

    Insert

    DmGW182_1-861-dsRNAres (gw Fly)
    

    Expression

    Insect

    Mutation

    Two non silent mutations V704A and N799S and one …

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAc5.1B-lambdaN-HA-DmGW182-del205-490-dsRNAres_R

    Plasmid

    #147409

    Purpose

    Insect Expression of DmGW182-del205-490-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

    Insert

    DmGW182-del205-490-dsRNAres (gw Fly)
    

    Expression

    Insect

    Mutation

    Two non silent mutations V704A and N799S and one …

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAc5.1B-EGFP-DmGW182_539-1384-dsRNAres_O

    Plasmid

    #147152

    Purpose

    Insect Expression of DmGW182_539-1384-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

    Insert

    DmGW182_539-1384-dsRNAres (gw Fly)
    

    Expression

    Insect

    Mutation

    Two non silent mutations V704A and N799S and one …

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAc5.1B-EGFP-DmGW182-Nterm6Wmut-SD6Wmut_O

    Plasmid

    #147154

    Purpose

    Insect Expression of DmGW182-Nterm6Wmut-SD6mut

    Depositor

    Elisa Izaurralde

    Article

    Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

    Insert

    DmGW182-Nterm6Wmut-SD6mut (gw Fly)
    

    Expression

    Insect

    Mutation

    Two non silent mutations V704A and N799S and one …

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. FUG-T2A-F241S

    Plasmid

    #140740

    Purpose

    Lentiviral vector to overexpress the autism-associated PTEN mutation F241S and GFP as two separate proteins

    Depositor

    Bryan Luikart

    Article

    Fricano-Kugler et al Biol Psychiatry. 2018 Aug 15;84(4):265-2

    Insert

    GFP-T2A-PTENF241S (PTEN Human)
    

    Use

    Lentiviral

    Tags

    none

    Expression

    Mammalian

    Mutation

    F241S

    Promoter

    hUbiC

    Available Since

    May 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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