We narrowed to 14,509 results for: TIM

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pYFP-lacY-1

Plasmid

#140138

Purpose

Evaluating the impact of feedback on information transmission of a genetic sensor.

Depositor

Drew Tack

Article

Sarkar et al Commun Biol. 2020 Apr 30;3(1):203. doi:

Insert

lacY (lacY )

Expression

Bacterial

Available Since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

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OeNL-H2B_pcDNA3

Plasmid

#89527

Purpose

Orange color variant of bright luminescent protein, fused to histone 2B

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

OeNL

Tags

histone 2B

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-cHAIAA7-spo5DSR

Plasmid

#49873

Purpose

for Cre-lox cassette exchange of C-terminal HA-auxin-inducible degron tagged gene sequences together with the S. pombe spo5 gene DSR element into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

IAA17 degron tag

Available Since

Jan. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pHFX-cas3UD

Plasmid

#174385

Purpose

Plasmids for gene knockout were constructed based on suicide plasmid pHFX

Depositor

Jing Han

Article

Lin et al Commun Biol. 2021 Aug 25;4(1):1007. doi:

Insert

Cas3UD

Use

Archaeal gene knockout vector for haloferax medit…

Available Since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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KA1130_pPB-TREtight-cHA-IRESH2BmCherry

Plasmid

#124173

Purpose

piggyBac-based Dox-inducible expression

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Expression

Mammalian

Available Since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-crRNA1

Plasmid

#123639

Purpose

Mammalian expression, Genome editing, gRNA scaffold

Depositor

Wei Li

Article

Teng et al Genome Biol. 2019 Feb 5;20(1):15. doi: 1

Insert

crRNA scaffold 1

Expression

Mammalian

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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RV-Syn-Dio-GFP

Plasmid

#87673

Purpose

retroviral vector encoding cre dependent expression of GFP

Depositor

Oscar Marin

Article

Lim et al Nat Neurosci. 2018 Jun 18. pii: 10.1038/

Insert

eGFP

Use

Retroviral

Promoter

Synapsin

Available Since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-shNup133-2

Plasmid

#87333

Purpose

To express shRNA against human Nup133

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup133

Use

RNAi

Expression

Mammalian

Promoter

H1

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pRSET FLII32Pglu-2.2m

Plasmid

#13566

Depositor

Wolf Frommer

Article

Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Insert

FLIPglu F16A P32S-ECFP-D33R

Tags

6xHis and Citrine

Expression

Bacterial

Mutation

F16A, ECFP insert in binding protein

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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OeNL-Actin_pcDNA3

Plasmid

#89524

Purpose

Orange color variant of bright luminescent protein, fused to actin

Depositor

Takeharu Nagai

Article

Suzuki et al Nat Commun. 2016 Dec 14;7:13718. doi: 10

Insert

OeNL

Tags

Actin

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv1.linker_KO

Plasmid

#164688

Purpose

For CRISPR knockout of miR-144~451 linker region by lentiviral delivery of Cas9 and linker gRNA

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Insert

miR-144~451 linker CRISPR KO gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pUCBB-lacZa

Plasmid

#32563

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

LacZ-alpha

Use

Synthetic Biology

Tags

His

Expression

Bacterial

Promoter

Consitutive lac

Available Since

Nov. 8, 2011

Availability

Academic Institutions and Nonprofits only

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pJ414 - DANCER-3

Plasmid

#136387

Purpose

Expresses DANCER-3 dynamic protein

Depositor

Roberto Chica

Article

Davey et al Nat Chem Biol. 2017 Dec;13(12):1280-1285

Insert

DANCER-3

Tags

6x-His tag (N-term of insert)

Expression

Bacterial

Mutation

Mutation from GB1 WT: Y3F-L7I-A34F-V39L-V54I

Promoter

T7 promoter

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pSox2-bd_mFGF_eGFP

Plasmid

#34704

Depositor

Hollis Cline

Article

Bestman et al J Comp Neurol. 2012 Feb 1;520(2):401-33.

Insert

eGFP

Use

Xenopus expression

Tags

165-bp minimal FGF4 promoter and 6x Sox2/Oct3-4 t…

Available Since

Feb. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pYPQ158

Plasmid

#69307

Purpose

Gateway entry vector with hSpCas9 (Human codon optimized)

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

hSpCas9 (Human codon optimized)

Use

CRISPR

Tags

3XFLAG

Mutation

NLS fusion

Available Since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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KA2706_eSpCas9-PGKPacdtkCh

Plasmid

#124204

Purpose

Expression of eSpCas9(1.1) and sgRNA

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Expression

Mammalian

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pYFP-x-1

Plasmid

#140142

Purpose

Evaluating information transmission of a genetic sensor.

Depositor

Drew Tack

Article

Sarkar et al Commun Biol. 2020 Apr 30;3(1):203. doi:

Insert

lacY (lacY )

Expression

Bacterial

Mutation

D37, I38, and N39 are changed to nonsense (stop) …

Available Since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

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pNB763

Plasmid

#60158

Purpose

Firefly luciferase (Promega) driven by LYS9 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc

Expression

Yeast

Mutation

FLuc has A4V & S504G (no functional effect)

Promoter

LYS9pr

Available Since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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B3rec.zf1

Plasmid

#108354

Purpose

Zebrafish codon optimized B3 recombinase that acts on blown-out sites

Depositor

Harold Burgess

Article

Tabor et al Curr Biol. 2018 Aug 20;28(16):2527-2535.

Insert

B3 recombinase

Use

Synthetic Biology

Mutation

Zebrafish codon optimized

Promoter

lac

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-XIAPmut

Plasmid

#8341

Depositor

Jon Ashwell

Article

Yang et al Science 2000 May 5;288(5467):874-7.

Insert

XIAPmut (XIAP Human)

Tags

GST

Expression

Bacterial

Mutation

H467A

Available Since

Nov. 15, 2005

Availability

Academic Institutions and Nonprofits only